Light Scattering Studies of Hydration and Structural Transformations of Lysozyme

• Authors: A. Szymańska , G. Ślósarek
• Languages of publication: EN

Abstracts

EN

We have applied the method of dynamic light scattering to analyse the lysozyme-ethanol interaction. For low ethanol concentration (below 4.3% (v/v)) no chemical denaturation process is observed. When the ethanol concentration grows above the triggering concentration the hydrodynamic radius of lysozyme increases, indicating the structural changes within the protein molecule. The observed structural modifications are attributed to dehydration and preliminary tertiary structure modification of the protein molecule.

Keywords

EN

87.15.kr; 34.50.-s

Discipline

• 34.50.-s: Scattering of atoms and molecules
• 87.15.kr: Protein-solvent interactions

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2012
• Volume: 121
• Issue: 3
• Pages: 694-698

Dates

published

2012-03

received

2010-10-07

(unknown)

2011-10-12

Contributors

author

A. Szymańska

• Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland

author

G. Ślósarek

• Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland

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Identifiers

DOI

10.12693/APhysPolA.121.694