PL EN


Preferences help
enabled [disable] Abstract
Number of results
2012 | 121 | 3 | 694-698
Article title

Light Scattering Studies of Hydration and Structural Transformations of Lysozyme

Content
Title variants
Languages of publication
EN
Abstracts
EN
We have applied the method of dynamic light scattering to analyse the lysozyme-ethanol interaction. For low ethanol concentration (below 4.3% (v/v)) no chemical denaturation process is observed. When the ethanol concentration grows above the triggering concentration the hydrodynamic radius of lysozyme increases, indicating the structural changes within the protein molecule. The observed structural modifications are attributed to dehydration and preliminary tertiary structure modification of the protein molecule.
Keywords
EN
Publisher

Year
Volume
121
Issue
3
Pages
694-698
Physical description
Dates
published
2012-03
received
2010-10-07
(unknown)
2011-10-12
Contributors
author
  • Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland
author
  • Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland
References
  • 1. M. Stefani, C.M. Dobson, J. Mol. Biol. 81, 678 (2003)
  • 2. V.N. Uversky, A.L. Fink, Biochim. Biophys. Acta 1698, 131 (2004)
  • 3. M.B. Pepys, P.N. Hawkins, D.R. Booth, D.M. Vigushin, G.A. Tennet, A.K. Soutar, N. Totty, O. Nguyen, C.C. Blake, C.J. Terry, T.G. Feest, A.M. Zalin, J.J. Hsuan, Nature 362, 553 (1993)
  • 4. A.J. Trexler, M.R. Nilsson, Curr. Prot. Pept. Sci. 8, 537 (2007)
  • 5. S. Goda, K. Takano, Y. Yamagata, R. Nagata, H. Akutsu, S. Maki, K. Namba, K. Yutani, Protein Sci. 9, 369 (2000)
  • 6. H.R.M. Krebs, K.D. Wilkins, W.E. Chung, C.M. Pitkeathly, K.A. Chamberlain, J. Zurdo, V.C. Ronibson, C.M. Dobson, J. Mol. Biol. 300, 541 (2000)
  • 7. A. Cao, D. Hu, L. Lai, Protein Sci. 13, 319 (2004)
  • 8. M. Holley, Ch. Eginton, D. Schaefer, L.R. Brown, Biochem. Biophys. Res. Commun. 373, 164 (2008)
  • 9. Y. Yonezawa, S. Tanaka, T. Kubota, K. Wakabayashi, K. Yutani, S. Fujiwara, J. Mol. Biol. 323, 237 (2002)
  • 10. B.J. Berne, R. Pecora, Dynamic Light Scattering, Wiley, New York 1976
  • 11. S.J. Perkins, Biophys. Chem. 93, 129 (2001)
  • 12. N. Javid, K. Vogtt, Ch. Krywka, M. Tolan, R. Winter, Chem. Phys. Chem. 8, 679 (2007)
  • 13. J. Garcia de la Tore, Biophys. Chem. 93, 159 (2001)
  • 14. R.E. Canfield, J. Biol. Chem. 238, 2698 (1963)
  • 15. M.C. Vaney, S. Maignan, M. Ries-Kautt, A. Ducruix, Acta Crystallogr. D 52, 505 (1996)
  • 16. A. Szymańska, Ph.D. Thesis, Adam Mickiewicz University, Poznań 2010
  • 17. A.J. Sophianopoulos, C.K. Rhodes, D.N. Holcomb, K.E. Van Holde, J. Biol. Chem. 237, 1107 (1962)
  • 18. S.B. Dubin, N.A. Clark, G.B. Benedek, J. Chem. Phys. 54, 5158 (1971)
  • 19. G. Chirico, S. Beretta, G. Baldini, J. Chem. Phys. 110, 2297 (1999)
  • 20. V. Calandrini, D. Fioretto, G. Onori, A. Santucci, Chem. Phys. Lett. 324, 344 (2000)
  • 21. S. Tanaka, Y. Oda, M. Ataka, K. Onuma, S. Fujiwara, Y. Yonezawa, Biopolymers 59, 370 (2001)
  • 22. J. Gierszewski, G. Ślósarek, Mol. Phys. Rep. 41, 81 (2005)
  • 23. W. Liu, D. Bratko, J.M. Prausnitz, H.W. Blanch, Biophys. Chem. 107, 289 (2004)
  • 24. A. Bonincontro, V. Calandrini, G. Onori, Colloids Surf. B 21, 311 (2001)
  • 25. D.F. Nicoli, G.B. Benedek, Biopolymers 15, 2421 (1976)
  • 26. S.B. Dubin, G. Feher, G.B. Benedek, Biochemistry 12, 714 (1973)
  • 27. Y. Georgalis, P. Umbach, J. Raptis, W. Saenger, Acta Crystallogr. D 53, 691 (1997)
  • 28. S. Arai, M. Hirai, Biophys. J. 76, 2192 (1999)
  • 29. M.S. Lehmann, S.A. Mason, G.J. McIntyre, Biochemistry 24, 5862 (1985)
  • 30. A. Deshpande, S. Nimsadkar, S.C. Mande, Acta Crystallogr. D 61, 1005 (2005)
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-a121z3p24kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.