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2012 | 19 | 1 | 39-46
Article title

Detection of Nitrate(NO-2) Ions Produced in Disproportionation of Nitrogen(II) Oxide in Aqueous Solution

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The nitrate ions (NO-2), products of disproportionation of NO in aqueous solution, were detected by an Orion Nitrite Electrode 97-46. Calibrations by means of standard NaNO2 solutions within the range 0.001÷100 ppm indicated linear dependence of EMF on ppm within 1÷100 ppm. Measurements justified the usefulness of this detection method of NO in solutions of OH- concentration lower than 10-2 mol dm-3 since at higher concentrations the EMF values exceeded the measurement range of the electrode. Occurrence of nitrate ions produced in the disproportionation reaction was additionally confirmed in dependence of OH- concentration by near UV and fluorescence spectra. The calibrated ion-selective nitrate electrode has also been shown, on the basis of Co(II)-dipeptide-OH- systems, as a useful tool in studying reversible NO uptake by Co(II) chelates in aqueous solution. Such a reaction may be regarded as simulating the harmful binding of NO by hemoglobin, where it substitutes the isoelectronic dioxygen.
Jony azotanowe (NO-2), produkty dysproporcjonowania NO w roztworze wodnym, oznaczano za pomocą jonoselektywnej elektrody Orion Nitride Electrode 97-46. Kalibracje za pomocą standardowych roztworów NaNO2 w zakresie 0.001÷100 ppm wskazywały liniową zależność SEM od ppm w zakresie 1÷100 ppm. Pomiary uzasadniły użyteczność tej metody wykrywania NO w roztworach o stężeniu OH- mniejszym niż 10-2 mol dm-3, ponieważ przy większych stężeniach wartości SEM przekraczały zakres pomiarowy elektrody. Występowanie jonów azotanowych tworzonych w reakcji dysproporcjonowania było dodatkowo potwierdzane, w zależności od stężenia OH-, za pomocą widm w bliskim UV i widm fluorescencyjnych. Wykazano także, na przykładzie układów Co(II)-dipeptyd-OH-, że kalibrowana jonoselektywna elektroda na jony azotanowe (NO-2) może być użytecznym narzędziem w badaniu odwracalnego wiązania NO przez chelaty Co(II) w roztworze wodnym, reakcji symulującej szkodliwe działanie tlenku azotu(II) na hemoglobinę.
Physical description
1 - 1 - 2012
8 - 11 - 2011
  • Department of Physical and Biocoordination Chemistry, Chair of Bioorganic and Biocoordination Chemistry, Faculty of Pharmacy, Medical University of Lodz, ul. Muszyńskiego 1, 90-151 Łódź, Poland
  • Department of Physical and Biocoordination Chemistry, Chair of Bioorganic and Biocoordination Chemistry, Faculty of Pharmacy, Medical University of Lodz, ul. Muszyńskiego 1, 90-151 Łódź, Poland
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