Introduction. The development of civilization is leading to lifestyle changes and diminishing physical activity as well as various types of sicknesses caused by a lack of movement. In order to reduce these negative effects we should maintain a proper level of physical activity, which is often regarded as a contributing factor to positive health levels. Material and st methods. The aim of this report is the assessment of physical fitness levels of 1st year male and female students of medicine and physiotherapy faculties at Lublin Medical University. The test was conducted on a group of 103 female and 52 male students of medicine as well as 50 female and 11 male students of physiotherapy. The survey was based on the Pilcz motor skills test and Denisiuk endurance test. Results and conclusions. The analysis of results showed differences in physical fitness between sexes while there were hardly any differences between the students of the two faculties. The overall level of physical fitness has been specified as the lower limit of the average level.