The purpose of this study was to quantify and compare asymmetry of stride length during 200 m sprint in different levels of performance. Six sprinters from national and regional levels participated in the study. They were assigned to 3 groups: school-boys (novice sprinters) junior (intermediate) and senior (advance - national and regional level) category. This study investigated selected kinematic parameters with special focus on stride length. The resulting values were measurements of each stride length (rounded-off to nearest full centimeter) during a 200 m sprint, using a manual stride measurement method. The findings indicate that the asymmetry of stride length exists in all categories, and the impact on decreasing velocities of the youngest sprinters (school-boys) are significantly associated with shorter strides, whereas cadence has little change. However, when a statistical adjustment was made for each group of runners it was found that more advanced runners did not have a significantly higher level of asymmetry with stride length at any given velocity.