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2008 | 19 | 93-108
Article title

Evaluation of the Blood Antioxidant Capacity in Two Selected Phases of the Training Cycle in Professional Soccer Players

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Abstracts
EN
The aim of this study was to evaluate the effects of a regular pre-season training on the aerobic performance and the blood antioxidant defense capacity in soccer players from the Polish Premier League club (n=19) and IVth League team (n=15). The players participated in an incremental treadmill running exercise to volitional fatigue twice (i.e., at the beginning (Trial A) and the end (Trial B) of the pre-season spring training). In venous blood samples, taken at rest and 3 min post-test, the activities of antioxidant enzymes (SOD, GSH-Px, CAT, GR) and concentrations of non-enzymatic antioxidants (GSH, tocopherols, retinol, uric acid) and malondialdehyde as a lipid peroxidation biomarker were measured. With the aim of between-group comparisons and possible conclusions on training-induced changes in the capacity of the blood antioxidant defense, the POTAX index was calculated as a sum of standardized activities of antioxidant enzymes and concentrations of non-enzymatic antioxidants.The results of the present study indicate that the players from the Premier League club were characterized by only slightly higher maximal oxygen uptake rates, the differences compared to IVth League team, as assessed in both trials, were statistically insignificant. Participation in the pre-season training resulted in a moderate improvement of aerobic performance, although only a few players were characterized by VO2max comparable to the international-class elite performers. No distinct differences were observed in the level of aerobic performance between higher- and lower-classified players.Pre-season training led to an improvement in the global blood antioxidant capacity expressed in terms of POTAOX indices, although the changes in the activities and concentrations of individual components of the antioxidant system were less pronounced. Training-induced level of antioxidant conditioning was higher among the Premier League players, which may be related to differences in the training schedule and nutritional preparation of the athletes.
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Year
Volume
19
Pages
93-108
Physical description
Dates
published
1 - 1 - 2008
online
24 - 10 - 2008
Contributors
  • Department of Biochemistry, Academy of Physical Education, Katowice
  • Department of Biochemistry, Academy of Physical Education, Katowice
  • Department of Biochemistry, Academy of Physical Education, Katowice
  • Department of Biochemistry, Academy of Physical Education, Katowice
  • Katowice School of Economics
  • Department of General and Analytical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Sosnowiec
author
  • University School of Physical Education, Wrocław
  • Department of Biochemistry, Academy of Physical Education, Katowice
  • Department of Biochemistry, Academy of Physical Education, Katowice
References
  • Aebi H. (1984) Catalase in vitro. Methods in Enzymolgy. 105:121[PubMed]
  • Agar N. S., Sadrzadeh M. H., Hallaway P. E., Eaton J. W. 1986. Erytrocyte catalase. A somatic oxidant defense? J Clin Invest. 77: 319-321[Crossref]
  • Banfi G., Malavazos A., Iorio E., Dolci A., Doneda L., Verna R., Corsi M. M. (2006) Plasma oxidative stress biomarkers. nitric oxide and heat shock protein 70 in elite soccer players. Eur J Appl Physiol. 96(5):483-486[PubMed][Crossref]
  • Bangsbo J., Nørregaard L., Thorsøe F. (1991) Activity profile of competition soccer. Can J Sport Sci. 16:110-61[PubMed]
  • Bangsbo J., Lindqvist F. (1992) Comparison of various exercise tests with endurance performance during soccer in professional players. Int J Sports Med. 13:125-132[PubMed][Crossref]
  • Bangsbo J. 1994. Energy demands in competitive soccer. J Sports Sci. 12: 183-189
  • Brites FD., Evelson PA., Christiansen M. G., Nicol M. F., Basilico M. J., Wikiński R. W., Llesuy S. F. (1999) Soccer players under regular training show oxidative stress but an improve plasma antioxidant status. Clin Sci. 96:381-385[Crossref]
  • Buege J. A., Aust S. D. (1978) Microsomal lipid peroxidation. Methods in Enzymology. 52:302-310[PubMed][Crossref]
  • Beutler E., Duron O., Kelly B. M. (1963) Improved method for the determination of blood glutathione. J Lab Clin Med. 61:882-888[PubMed]
  • Cazzola R., Russo-Volpe S., Cervato G., Cestaro B. (2003) Biochemical assessments of oxidative stress. erythrocyte membrane fluidity and antioxidant status in professional soccer players and sedentary controls. Eur J Clin Invest. 33(10)924-930[PubMed][Crossref]
  • Dahlgren C., Karlsson A. 1999. Respiratory burst in human neutrophils. J Immunol Methods. 232:3-14[Crossref][PubMed]
  • Danch A., Dróżdż M. (1996) Uproszczona metoda fluorometrycznego oznaczania selenu w materiale biologicznym. Diagn Lab. 32:5729-5734
  • Ferreira L. F., Reid M. B. 2008. Muscle-derived ROS and thiol regulation in muscle fatigue. J Appl Physiol. 104:853-860[PubMed][Crossref]
  • Finaud J., Lac G., Filarie E. (2006) Oxidative stress. Relation with exercise and training. Sports Med. 36(4):327-358[Crossref]
  • Glatzle D., Korner W. F., Christeller S., Wiss O. (1970) Method for the detection of a biochemical riboflavin deficiency. Stimulation of NADPH 2-dependent glutathione reductase from human erythrocytes by FAD in vitro. Investigations on the vitamin B2 status in healthy people and geriatric patients. Int Vit Res. 40(2):166-183
  • Helgerud J., Engen L. C., Wisløff U., Hoff J. (2001) Aerobic endurance training improves soccer performance. Med Sci Sports Exerc. 33: 1925-1931[PubMed][Crossref]
  • Hoff J., Helgerud J. (2004) Endurance and Strength Training for Soccer Players. Sports Med. 34(3): 165-180[Crossref][PubMed]
  • Hoff J. (2005) Training and testing physical capacities for elite soccer players. J Sports Sci, 23(6): 573-582[Crossref][PubMed]
  • Jenkins R., Friedland J., Holland H. (1994) The relationship of oxygen uptake to superoxide dismutase and catalase activity in human skeletal muscle. Int Sci Sports Med. 5:11-14
  • Janaszewska A., Bartosz G. 2002. Assay of total antioxidant capacity: comparison of four methods as applied to human plasma. Scand J Clin Lab Invest. 62: 231-236[PubMed][Crossref]
  • Ji LL. (1999) Antioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med. 222(3): 283-292[Crossref][PubMed]
  • Kemi O. J., Hoff J., Engen L. C., Helgerud J., Wisløff U. (2003) Soccer specific testing of maximal oxygen uptake. J Sports Med Phys Fitness. 43:139-144[PubMed]
  • Kłapcińska B., Poprzęcki S., Danch A., Sobczak A., Kempa K. (2005) Selenium levels in blood of Upper Silesian population: evidence of suboptimal selenium status in a significant percentage of the population. Biol Trace Elem Res Winter 108: 1-15[Crossref]
  • Mastaloudis A., Traber M. G., Carstensen K., Widrick J. J. (2006) Antioxidants did not prevent muscle damage in response to an ultramarathon run. Med Sci Sports Exerci. 38(1):72-80[Crossref]
  • Maxwell S. R., Dietrich T., Chapple I. L. C. (2006) Prediction of serum total antioxidant activity from the concentration of individual serum antioxidants. Clin Chim Acta. 372: 188-194
  • McMillan K., Helgerud J., Macdonald R., Hoff J. (2005) Physical adaptations to soccer specific endurance training in professional youth soccer players. Br J Sports Med. 39:273-277[Crossref][PubMed]
  • Nielsen F., Mikkelsen B. B., Nielsen J. B., Andersen H. R., Grandjean P. 1997. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem. 43:1207-1214
  • Ohno H., Yahata T., Sato Y., Yamamura K., Taniguchi N. (1988) Physical training and fasting erythrocyte activities of free radical scavenging enzyme system in sedentary men. Eur J Appl Physiol Occup Physiol. 57:173-176[Crossref]
  • Pattwell D. M., Jackson M. J. (2004) Contraction-induced oxidants as mediators of adaptation and damage in skeletal muscle. Exerc Sport Sci Rev. 32: 14-18[Crossref][PubMed]
  • Poprzęcki S. (2003) The effect of omega-3 fatty acid and α-tocopherol supplementation on physical work capacity and blood antioxidant status in male subject. J Hum Kinetics. 10:121-136
  • Reid M. B. (2001) Plasticity in skeletal, cardiac, and smooth muscle. Invited review: Redox modulation of skeletal muscle contraction: what we know and what we don't. J Appl Physiol. 90: 724-731
  • Reilly T., Drust B., Clarke N. (2008) Muscle fatigue during football match-play. Sports Med. 38: 357-367[PubMed][Crossref]
  • Rice-Evans C. A., Diplock A. T., Symons M. C. R. (1991) Techniques in Free Radical Research, Elsevier, Amsterdam 148-149
  • Salo D. C., Pacifici R. E., Lin S. W., Giulivi C., Davies K. J. (1990) Superoxide dismutase undergoes proteolysis and fragmentation following oxidative modification and inactivation. J Biol Chem. 265: 11919-11927[PubMed]
  • Shippinger G., Wonish P. M., Fankhauser F., Winklhofer-Roob B. M., Halwachs G. (2002) Lipid peroxidation and antioxidant status in professional American football players during competition. Eur Journal of Clin Invest. 32:686-692[Crossref]
  • Sjödin B., Hellsten Y., Apple F. S., (1990) Biochemical mechanisms for oxygen free radical formation during exercise. Sports Med. 10: 236-254[Crossref][PubMed]
  • Sobczak A., Skop B., Kula B. (1999) Simultaneus determination of serum retinol and alpha- and gamma- tocopherol levels in type II diabetics patients using high- performance liquid chromatography with fluorescence detection. J Chromatogr B Biomed Sci Appl. 730:265-271
  • Stølen T., Chamari K., Castagna C., Wisløff U. 2005. Physiology of soccer. An update. Sports Med. 35: 501-536[PubMed][Crossref]
  • Strøyer J., Hansen L., Klausen K. (2004) Physiological profile and activity pattern of young soccer players during match play. Med Sci Sports Exerc. 36:168-174[PubMed][Crossref]
  • Svensson M., Drust B. (2005) Testing soccer players. J Sports Sci. 23:601-618[Crossref][PubMed]
  • Thomson C. D. 2004. Assessment of requirements for selenium and adequacy of selenium status: a review. Eur J Clin Nutr. 58: 391-402[PubMed][Crossref]
  • Viña J., Gomez-Cabrera M. C., Lloret A., Marquez R., Minana J. B., Pallardo F. V., Sastre J. (2000) Free radicals in exhaustive physical exercise: mechanism of production. and protection by antioxidant. IUBMB Life 50(4-5):271-277[PubMed][Crossref]
  • Vollaard N. B. J., Sherman J. P., Cooper C. E. (2005) Exercise-Induce Oxidative Stress. Myths. Realities and Physiological Relevance. Sports Med. 35(12):1045-1062[Crossref][PubMed]
  • Wisløff U., Helgerud J., Hoff J. (1998) Strength and endurance of elite soccer players. Med Sci Sports Exerc. 30(3):462-467[PubMed][Crossref]
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_v10078-008-0007-9
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