Purpose. Previous research has shown that physical activity (PA) is determined by several variables, such as gender, social economic condition (SES) and place of residence. The main purpose of this study was to study the association between education and PA of the Czech adult population as well as discovering any other socio-demographic factors that may influence PA. Methods. A population-based survey conducted in 2008 resulted in 6,989 International Physical Activity Questionnaires (short version) from Czech adults aged 26-69 years. This survey included all regions in the Czech Republic. The data were analysed using frequencies and binomial logistic regression separately for gender and education level. The dependent variables were classified as either the "healthy minimum" and "health promotion" according to the amount of PA criteria the individuals met. Results. People with a university education had less PA than other groups of different education levels. The "health promotion" category was met by 9.9% of women and 6.5% of men with elementary education, 67.4% of women and 71.3% of men with a secondary education, and 22.7% of women and 22.2% of men with a university education. The "health promotion" category is also more likely to be met by males (OR 1.33, CI 1.20-1.48, p < 0.001), people with elementary (OR 1.67, CI 1.36-2.06, p < 0.001) and secondary education (OR 1.60, CI 1.42-1.80, p < 0.001), those living with a family with children (OR 1.49, CI 1.07-1.53, p < 0.001), living in villages (OR 1.35, CI 1.14-1.60, p < 0.001) or small towns (OR 1.27, CI 1.10-1.61, p < 0.001), those who have a dog (OR 1.15, CI 1.04-1.27, p < 0.05), and those who participate in organized PA (OR 1.30, CI 1.17-1.44, p < 0.001). Conclusions. There was a surprising low amount of PA among those who studied at a university. Programs that promote PA among university students and future graduates should be considered.