Purpose. The purpose of this study was to investigate the effects of different exercise orders on the local muscular endurance of trained women. Methods. Nineteen women with a minimum of two years experience in resistance training volunteered to participate in the study (age 27.68 ± 5.24 years; body mass 60.31 ± 7.50 kg; height 161.83 ± 7.05 cm; body mass index 22.85 ± 1.85 kg · m-2). Data were collected in two phases: 1) determining the one repetition maximum (1RM) for the bench press (BP), machine lat pull-down (LPD), free-weight shoulder press (SP), standing free-weight biceps curl (BC), and triceps extension (TE); 2) the completion of two resistance training sequences including 4 sets of exercise at 60% of 1RM with 2 minute rest intervals between sets with exercises performed until failure: Sequence A (SEQ A) comprised of: BP, LPD, SP, BC, TE while sequence B (SEQ B): TE, BC, SP, LPD, BP. Results. The mean number of repetitions per set in BP and TE presented significant reductions (p = 0.001 and p = 0.026, respectively) when they were the last exercise performed in each exercise sequence. Rating of Perceived Exertion (RPE) was not significantly different between the exercise sequences; however, increases for BC (in SEQ A) and BP (in SEQ B) were observed when they were performed later in the sequences. Conclusion. These data indicate that in trained women, local muscular endurance is affected by exercise sequence, with exercises performed later in a workout sequence showing decreased exercise ability due to fatigue.