Genetic Variations of the CTNNA1 And The CTNNB1 Genes in Sporadic Colorectal Cancer in Polish Population

• Authors: Andrzej Sygut , Karolina Przybyłowska , Tomasz Ferenc , Łukasz Dziki , Michał Spychalski , Michał Mik , Adam Dziki
• Languages of publication: EN

Abstracts

EN

Experimental as well as clinical observations have demonstrated that the E-cadherin/catenin complex is a powerful inhibitor of invasion. Abrogation of this pathway is implicated in the carcinogenesis of several malignancies, especially colorectal cancer.The aim of the study was to determine the CTNNA1 and the CTNNB1 mutations and its relationship to clinical and pathological features of sporadic colorectal cancer (CRC) in Polish patients.Material and methods. Paired tumor and normal tissue samples from 110 sporadic CRC patients undergoing resective surgery were prospectively studied for the alpha catenin (CTNNA1) gene and beta catenin (CTNNB1)gene mutations by PCR/single strand conformation polymorphism (SSCP).Results. The CTNNA1 gene alteration in exon 7 were detected in 4 samples and in exon 3 of CTNNB1 gene were found in 3 samples. There was a trend at the limit of statistical significance associating younger age at diagnosis (<50) with CTNNA1 and the CTNNB1 mutations. The mutation of CTNNB1 seemed to occur more frequently in the proximal colon than distal. The CRC patients with CTNNA1 mutation had a significantly increased lymph node metastasis. On the other hand, there was no correlation between mutations and the other clinical variables (e.g. sex, grade and depth of invasion).Conclusion. Although we found a low frequency of mutations in the CTNNA1 and the CTNNB1 genes, but the analysis the relationship with clinical and pathological features of CRC patients may indicated an association of these mutations with the risk and progression of CRC.

Keywords

EN

sporadic colorectal cancer; muations; catenin gene; adhesion molecules

• Journal: Polish Journal of Surgery
• Year: 2012
• Volume: 84
• Issue: 11
• Pages: 560-564

Dates

published

1 - 12 - 2012

online

09 - 02 - 2013

Contributors

author

Andrzej Sygut

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Karolina Przybyłowska

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Tomasz Ferenc

• Department of Biology and Medical Genetics, Medical University in Łódź

author

Łukasz Dziki

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Michał Spychalski

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Michał Mik

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Adam Dziki

• Department of General and Colorectal Surgery, Medical University in Łódź

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Identifiers

DOI

10.2478/v10035-012-0093-1