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2012 | 84 | 7 | 358-362
Article title

Lack of Association Between the 135G/C Rad51 Gene Polymorphism and the Risk of Colorectal Cancer Among Polish Population

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One of the major causes of carcinogenesis is loss of genome stability. RAD51 in process of homologous recombination (HR) played crucial role in maintenance integrity of genome through initiate of DNA double strand breaks repair. Presence of single nucleotide polymorphism (SNP) in RAD51 gene could change the capacity of DNA repair and altered the response to damaging agents. Research on potential impact of genetic variability on development and progression CRC may contribute to setting new genetic markers or/and determined individual susceptibility to CRC.The aim of the study. This study was designed to evaluate the effect of 135 G/C (rs1801320) RAD51 polymorphism located in the 5' untraslated region on the risk and progression of CRC.Material and methods. The subjects consisted of histologically confirmed colorectal cancer (n = 200) and controls (n = 200) with lack of previous history of cancer. The distribution of genotypes was determined by restriction fragment length polymorphism PCR (RFLP - PCR). Statistical analysis was based on multivariate regression model.Results and conclusion. Our study reveal no significance association of 135 G/C RAD51 polymorphism with occurrence and progression of colorectal cancer.
Physical description
1 - 7 - 2012
30 - 8 - 2012
  • Department of Clinical Chemistry and Biochemistry, Medical University in Łódź
  • Department of Clinical Chemistry and Biochemistry, Medical University in Łódź
  • Department of General and Colorectal Surgery, Medical University in Łódź
  • Department of General and Colorectal Surgery, Medical University in Łódź
  • Department of General and Colorectal Surgery, Medical University in Łódź
  • Department of Clinical Chemistry and Biochemistry, Medical University in Łódź
  • Ferlay J, Parkin DM, Steliarova-Foucher E: Estimates of cancer incidence and mortality in Europe in 2008. Eur J Cancer 2010; 46(4): 765-81.[PubMed][Crossref]
  • Parkin DM, Bray F, Ferlay J et al.: Global cancer statistics, 2002. CA Cancer J Clin 2005; 55(2): 74-108.
  • Poynter JN, Gruber SB, Higgins PD et al.: Statins and the risk of colorectal cancer. N Engl J Med 2005; 352(21): 2184-92.
  • Potter JD: Colorectal cancer: molecules and populations. J Natl Cancer Inst 1999; 91(11): 916-32.
  • Weitz J, Koch M, Debus J et al.: Colorectal cancer. Lancet 2005; 365(9454): 153-65.
  • Elliott B, Jasin M: Double-strand breaks and translocations in cancer. Cell Mol Life Sci 2002; 59(2): 373-85.[PubMed][Crossref]
  • Khanna KK, Jackson SP: DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet 2001; 27(3): 247-54.[Crossref][PubMed]
  • Harrison JC, Haber JE: Surviving the breakup: the DNA damage checkpoint. Annu Rev Genet 2006; 40: 209-35.[WoS]
  • Johnson R, D, Jasin M: Sister chromatid gene conversion is a prominent double-strand break repair pathway in mammalian cells. The EMBO journal 2000; 19(13): 3398-3407.[Crossref][PubMed]
  • Carreira A, Kowalczykowski SC: Two classes of BRC repeats in BRCA2 promote RAD51 nucleoprotein filament function by distinct mechanisms. Proc Natl Acad Sci USA 2011; 108(26): 10448-53.
  • Li XHeyer WD: Homologous recombination in DNA repair and DNA damage tolerance. Cell Res 2008; 18(1): 99-113.
  • Baumann P, West SC: Role of the human RAD51 protein in homologous recombination and double-stranded-break repair. Trends Biochem Sci 1998; 23(7): 247-51.[Crossref]
  • San Filippo J, Sung P, Klein H: Mechanism of eukaryotic homologous recombination. Annu Rev Biochem 2008; 77: 229-57.[WoS]
  • Schmutte C, Tombline G, Rhiem K et al.: Characterization of the human Rad51 genomic locus and examination of tumors with 15q14-15 loss of heterozygosity (LOH). Cancer Res 1999; 59(18): 4564-69.
  • Tsuzuki T, Fujii Y, Sakumi K et al.: Targeted disruption of the Rad51 gene leads to lethality in embryonic mice. Proc Natl Acad Sci USA 1996; 93(13): 6236-40.
  • Wang WW, Spurdle AB, Kolachana P et al.: A single nucleotide polymorphism in the 5' untranslated region of RAD51 and risk of cancer among BRCA1/2 mutation carriers. Cancer Epidemiol Biomarkers Prev 2001; 10(9): 955-60.
  • Hasselbach L, Haase S, Fischer D et al.: Characterisation of the promoter region of the human DNA-repair gene Rad51. Eur J Gynaecol Oncol 2005; 26(6): 589-98.
  • Zhou GW, Hu J, Peng XD et al.: RAD51 135G>C polymorphism and breast cancer risk: a meta-analysis. Breast Cancer Res Treat 2011; 125(2): 529-35.
  • Levy-Lahad E, Lahad A, Eisenberg S et al.: A single nucleotide polymorphism in the RAD51 gene modifies cancer risk in BRCA2 but not BRCA1 carriers. Proc Natl Acad Sci USA 2001; 98(6): 3232-36.[Crossref]
  • Helleday T: Homologous recombination in cancer development, treatment and development of drug resistance. Carcinogenesis 2010; 31(6): 955-60.[WoS][Crossref][PubMed]
  • Gray NK: Translational control by repressor proteins binding to the 5'UTR of mRNAs. Methods Mol Biol 1998; 77: 379-97.
  • Arias-Lopez C, Lazaro-Trueba I, Kerr P et al.: p53 modulates homologous recombination by transcriptional regulation of the RAD51 gene. EMBO Rep 2006; 7(2): 219-24.
  • Wiśniewska-Jarosińska M, Śliwinski T, Krupa R et al.: The role of RAD 51 gene polymorphism in patients with colorectal cancer in the Polish subpopulation. Pol Merkur Lekarski 2009; 26(155): 455-57.
  • Krupa R, Sliwinski T, Wiśniewska-Jarosińska M et al.: Polymorphisms in RAD51, XRCC2 and XRCC3 genes of the homologous recombination repair in colorectal cancer - a case control study. Mol Biol Rep 2011; 38(4): 2849-54.
  • Poplawski T, Arabski M, Kozirowska D et al.: DNA damage and repair in gastric cancer-a correlation with the hOGG1 and RAD51 genes polymorphisms. Mutat Res 2006; 601(1-2): 83-91.
  • Jakubowska A, Gronwald J, Menkiszak J et al.: The RAD51 135 G>C polymorphism modifies breast cancer and ovarian cancer risk in Polish BRCA1 mutation carriers. Cancer Epidemiol Biomarkers Prev 2007; 16(2): 270-75.[WoS]
  • Rollinson S, Smith AG, Allan JM et al.: RAD51 homologous recombination repair gene haplotypes and risk of acute myeloid leukaemia. Leuk Res 2007; 31(2): 169-74.[WoS]
  • Fang F, Wang J, Yao L et al.: Relationship between XRCC3 T241M polymorphism and gastric cancer risk: a meta-analysis. Medical oncology (Northwood, London, England), 2011; 28(4): 999-1003.[WoS]
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