PL EN


Preferences help
enabled [disable] Abstract
Number of results
2012 | 84 | 6 | 271-275
Article title

Higher Apoptosis Index and Proliferation Index in Colonocytes of Patients with Ulcerative Colitis in Remission

Content
Title variants
Languages of publication
EN
Abstracts
EN
Ulcerative colitis (UC) is a inflammatory disease of large bowel. The amount of people suffering from UC increases from year to year. Pathogenesis of this affection is still not entirely clear. Mechanisms of proliferation and apoptosis in colonocytes in the course of the disease are defectedThe aim of the study was to assess the rate of proliferation and intensity of apoptosis in colonocytes in patients with diagnose UC.Material and methods. Colon pathological samples taken from patients with diagnosed ulceraive colitis were examined. Patients were in both clinical and endoscopic remission and were treated with mesalazin. They were patient of Department of General and Colorectal Surgery. To estimate proliferation index dye with monoclonal antibody against Ki67. To determine apoptosis level immunohistochemistry with antybody against Bax was used.Results. Average Ki-67 in the test group was 42,13%, the largest value amounted to 57% and the lowest of 33%. Average value of Bax was 1.47 and ranged between 0-3. High index of bax appear not only in the bottom of the crypt, but also at their outlet.Conclusions. In ulecerative colitis genetic and immunological disturbances occur despite treatment. Mesalazine acting only on certain routes associated with the UC holds the remission, without, however "the molecular remission". Thus, it appears that the results of our research are another proof of the necessary caution in weaning support treatment.
Publisher

Year
Volume
84
Issue
6
Pages
271-275
Physical description
Dates
published
1 - 6 - 2012
online
28 - 7 - 2012
Contributors
  • Department of General and Colorectal Surgery, Medical University in Łódź
  • Department of General and Colorectal Surgery, Medical University in Łódź
  • Department of Pathomorphology, Medical Uniersity in Łódź
author
  • Department of General and Colorectal Surgery, Medical University in Łódź
References
  • Ekbom A, Helmick CZack M, Adami HO: The epidemiology of inflammatory bowel disease: a large, population-based study in Sweden. Gastroenterology 1991; 100(2): 350-58.[PubMed]
  • Haruta J, Kusugami K, Kuroiwa A et al.: Phenotypic and functional analysis of lamina propria mononuclear cells from colonoscopic biopsy specimen in patients with ulcerative colitis. Am. J Gastroenterology 1992; 87: 448-54.
  • Rosai J: Ackerman's Surgical Pathology. Mosbby 1996; 667-799.
  • Fisher SA, Mark Tremelling M, Anderson CA et al.: Genetic determinants of ulcerative colitis include the ECM1 locus and five loci implicated in Crohn's disease Nat Genet 2008 June; 40(6): 710-12.[WoS]
  • Mansfield JC, Holden H, Tarlow JK et al.: Novel genetic association between ulcerative colitis and the anti-inflammatory cytokine interleukin-1 receptor antagonist. Gastroenterology 1994; 106(3): 637-42.[PubMed]
  • Wei Qiu, Bin Wu1, Xinwei Wang et al.: PUMA-mediated intestinal epithelial apoptosis contributes to ulcerative colitis in humans and mice. J Clin Invest 2011; 121(5): 1722-32.[WoS]
  • Michiko Iwamoto, Takehiko Koji, Kazuya Makiyama et al.: Apoptosis Of Crypt Epithelial Cells In Ulcerative Colitis. Journal of Pathology 1996 Oct. 180(2): 152-59.
  • Kornbluth A, Sachar DB: Ulcerative Colitis Practice Guidelines in Adults: American College of Gastroenterology, Practice Parameters Committee. Am J Gastroenterol 2010; 105:501-23.[WoS]
  • Edelblum KL, Yan F, Yamaoka P et al.: Regulation of apoptosis during homeostasis and disease in the intestinal epithelium. Inflamm Bowel Dis 2006; 12(5): 413-24.[Crossref]
  • Iwamoto M, Koji T, Makiyama K et al.: Apoptosis of crypt epithelial cells in ulcerative colitis. J of pathology 1996; 180: 152-59.
  • Ru Chen, Rabinovitch PS, Crispin DA et al.: The initiation of colon cancer in a chronic inflammatory setting. Carcinogenesis 2005; 26(9): 1513-19.[Crossref]
  • Vetuschi A, Latella G, Sferra R et al.: Increased Proliferation and Apoptosis of Colonic Epithelial Cells in Dextran Sulfate Sodium-Induced Colitis in Rats. Digestive Diseases and Sciences 2002; 47(7): 1447-57.[Crossref][PubMed]
  • van Staa TP, Card1T, Logan RF et al.: 5-Aminosalicylate use and colorectal cancer risk in inflammatory bowel disease: a large epidemiological study. Gut 2005 Nov; 54(11): 1573-78.
  • Chu EC, Chai J, Ahluwalia A et al.: Mesalazine downregulates c-Myc in human colon cancer cells. A key to its chemopreventive action? Aliment Pharmacol Ther 2007 Jun 15; 25(12): 1443-49.[WoS]
  • Eaden J: The data supporting a role for aminosalicylates in the chemoprevention of colorectal cancer in patients with inflammatory bowel disease. Aliment Pharmacol Ther 2003 Sep; 18 Suppl 2: 15-21.[PubMed][Crossref]
  • Isao Okayasu, Kiyomi Hana, Tsutomu Yoshida et al.: Significant Increase of Colonic Mutated Crypts in Ulcerative Colitis Correlatively with Duration of Illness. Cancer Res 2002 April 15; 62: 2236-38.
  • Reinacher-Schick A., Schoeneck A., Graeven U et al.: Mesalazine causes a mitotic arrest and induces caspase-dependent apoptosis in colon carcinoma cells. Carcinogenesis 2003; 24(3): 443-51.[Crossref]
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_v10035-012-0046-8
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.