Association of -1112 C/T Promoter Region Polymorphism of the Interleukin 13 Gene with Occurrence of Colorectal Cancer

• Authors: Anna Walczak , Karolina Przybyłowska , Radzisław Trzciński , Andrzej Sygut , Łukasz Dziki , Adam Dziki , Ireneusz Majsterek
• Languages of publication: EN

Abstracts

EN

Colorectal carcinoma is one of the leading causes of death from cancer amongst adults. Considering its molecular background, cytokines are the key component of the inflammatory microenvironment of these tumors. Investigations that enable better understanding of colorectal cancer concerning the molecular level, may provide important tools for genetic screening of disease high-risk groups, as well as molecular diagnostics for the non-invasive detection of cancer in its early stages.The aim of the study was to evaluate the association between colorectal cancer and the -1112 C/T single nucleotide polymorphism (SNP) of the interleukin-13 gene.Material and methods. The study group comprised 150 cancer patients and 170 healthy subject genotypes from the Polish population. Analysis was performed by PCR-restriction fragment length polymorphism (PCR-RFLP).Results. We showed that the CT genotype is connected with a higher risk of colon cancer occurrence (OR 2.51; 95% CI 1.57-4.02; p < 0.0001). We also correlated the polymorphic variants of the IL-13 gene with the clinical characteristics of colorectal cancer patients. We observed no association between the investigated polymorphism and colorectal cancer progression, evaluated by tumor stage, as well as lymph node metastasis.Conclusions. The presented study suggested the possibility of a connection between the IL-13 gene polymorphism (-1112 C/T) and colorectal cancer risk in the Polish population.

Keywords

EN

colorectal cancer; IL-13 gene polymorphism; cytokines

• Publisher: De Gruyter Open
• Journal: Polish Journal of Surgery
• Year: 2011
• Volume: 83
• Issue: 1
• Pages: 27-31

Dates

published

1 - 1 - 2011

online

14 - 3 - 2011

Contributors

author

Anna Walczak

• Department of Clinical Chemistry and Biochemistry, Medical University in Łódź

author

Karolina Przybyłowska

• Department of Clinical Chemistry and Biochemistry, Medical University in Łódź

author

Radzisław Trzciński

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Andrzej Sygut

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Łukasz Dziki

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Adam Dziki

• Department of General and Colorectal Surgery, Medical University in Łódź

author

Ireneusz Majsterek

• Department of Clinical Chemistry and Biochemistry, Medical University in Łódź

References

• Raporty na podstawie danych Centrum Onkologii -
• Kemp Z, Thirlwell C, Sieber O et al.: An update on the genetics of colorectal cancer. Hum Mol Genet 2004; 13, 177-85.
• Minty A, Ferrara P, Caput D: Interleukin-13 effects on activated monocytes lead to novel cytokine secretion profiles intermediate between those induced by interleukin-10 and by interferon-gamma. Eur Cytokine Netw 1997; 8: 189-201.
• Hajoui O, Janani R, Tulic M et al.: Synthesis of IL-13 by human B lymphocytes: regulation and role in IgE production. J Allergy Clin Immunol 2004; 114: 657-63.
• Endo Y, Marusawa H, Kou T et al.: Activation-induced cytidine deaminase links between inflammation and the development of colitis-associated colorectal cancers. Gastroenterology 2008; 135: 889-98.
• Rothenberg ME: Biology and treatment of eosinophilic esophagitis. Gastroenterology 2009; 137: 1238-49.
• Formentini A, Prokopchuk O, Sträter J et al.: Interleukin-13 exerts autocrine growth-promoting effects on human pancreatic cancer, and its expression correlates with a propensity for lymph node metastases. Int J Colorectal Dis 2009; 24: 57-67.[WoS]
• Faghih Z, Erfani N, Razmkhah M et al.: Interleukin13 haplotypes and susceptibility of Iranian women to breast cancer. Mol Biol Rep 2009; 36: 1923-28.[WoS]
• Sameni S, Ghayumi MA, Mortazavi G et al.: Lack of association between interleukin-13 gene polymorphisms (-1055 C/T and +2044 G/A) in Iranian patients with lung cancer. Mol Biol Rep 2009; 36: 1001-05.
• Schwartzbaum JA, Ahlbom A, Lönn S et al.: M. An international case-control study of interleukin- 4Ralpha, interleukin-13, and cyclooxygenase-2 polymorphisms and glioblastoma risk. Cancer Epidemiol Biomarkers Prev 2007; 16: 2448-54.[WoS]
• Mandal D, Levine AD: Elevated IL-13 Ralpha2 in intestinal epithelial cells from ulcerative colitis or colorectal cancer initiates MAPK pathway. Inflamm Bowel Dis 2010; 16: 753-64.
• Murata T, Noguchi PD, Puri RK: IL-13 induces phosphorylation and activation of JAK2 Janus kinase in human colon carcinoma cell lines: similarities between IL-4 and IL-13 signaling. J Immunol 1996; 156: 2972-78.
• Kanai T, Watanabe M, Hayashi A et al.: Regulatory effect of interleukin-4 and interleukin-13 on colon cancer cell adhesion. Br J Cancer 2000; 82: 1717-23.
• Trejdosiewicz LK, Morton R, Yang Y et al.: Interleukins 4 and 13 upregulate expression of cd44 in human colonic epithelial cell lines. Cytokine 1998; 10: 756-65.

Identifiers

DOI

10.2478/v10035-011-0004-x