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2010 | 82 | 1 | 47-52

Article title

Expression of Genes Encoding Aldosterone Synthesis Pathway Enzymatic Proteins in Adrenocortical Adenomas in Patients With Conn Syndrome

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Languages of publication

EN

Abstracts

EN
One of the possibilities of explanation of persistent hypertension after adrenocortical adenoma excision in patients with Conn syndrome could be analysis of transcriptional activity of genes encoding aldosterone synthesis pathway enzymatic proteins within these tumours.The aim of the study was to analyze the expression of mentioned genes in adrenocortical adenomas in patients with primary hiperaldosteronism and to analyse correlations between their expression and results of hormonal tests of blood serum before surgery as well as maximal values of blood pressure before surgery and their decrease after operation.Material and methods. Thirteen patients with Conn syndrome subjected to surgical excision of adrenocortical tumour were analyzed. Expression of CYP 11A1, CYP 21A2, CYP 11B2 and HSD 3B2 genes in tissue of removed tumours was analyzed using RT real-time PCR method. GAPDH was used as reference gene. The results analysis was based on amplification threshold values CT. Analysis of clinical consequences of adrenalectomy was performed after a period of minimum 12 months after surgery.Results. Expression of mRNA, presented as 2-ΔΔCT, of CYP 11A1 gene was 1.58 (1.01-2.48), CYP 21A2-2.74 (1.78-4.24), CYP 11B2- 19.15 (11.28-32.50) and HSD 3B2-2.57 (1.47-4.50). Correlation analysis revealed only negative correlation between CYP 11A1 gene expression and plasma renin activity in upright position (r=-0.604; p=0.049) and positive correlation between HSD 3B2 gene expression and decrease of maximal values of systolic blood pressure in long term follow up (r=0.763; p=0.010).Conclusions. High expression of genes encoding aldosterone synthesis pathway enzymatic proteins is observed in adrenocortical adenomas in patients with Conn syndrome. The transcriptional activity of the genes does not correlate with results of preoperative hormonal tests and is not a prognostic factor of persistent hypertension after adrenal tumour excision.

Year

Volume

82

Issue

1

Pages

47-52

Physical description

Dates

published
1 - 1 - 2010
online
10 - 2 - 2010

Contributors

author
  • Department of General, Vascular and Transplant Surgery, Silesian Medical University, Katowice
  • Department of Biochemistry, Silesian Medical University, Katowice
author
  • Department of Pathophysiology, Silesian Medical University, Katowice
author
  • Department of General, Vascular and Transplant Surgery, Silesian Medical University, Katowice
author
  • Department of Morphology, Silesian Medical University, Katowice
  • Department of Nephrology, Endocrinology and Metabolic Diseases, Silesian Medical University, Katowice
  • Department of Molecular Biology, Silesian Medical University, Katowice
author
  • Department of General, Vascular and Transplant Surgery, Silesian Medical University, Katowice

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_v10035-010-0008-y
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