Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl


Preferences help
enabled [disable] Abstract
Number of results
2009 | 81 | 12 | 595-602

Article title

Dysplasia and Microsatellite DNA Instability in Colorectal Adenomas


Title variants

Languages of publication



Microsatellite DNA instability (MSI) is a consequence of disorder within mismatch repair genes coding DNA repair proteins, protecting the cell against replication errors. Their dysfunction leads to gathering of adverse mutations within a cell, which may result in its neoplastic transformation.The aim of the study was to analyse the occurrence of microsatellite DNA instability in polypoid adenomas of large intestine removed during endoscopic polypectomy.Material and methods. The study covered 97 patients (30 women and 67 men), who underwent colonoscopic polypectomy in Endoscopy Ward, 2nd Department of Surgery, Jagiellonian University, Medical College in Cracow, between 2004 and 2007. Sampled materials was verified histopathologically and genetic tests were performed with the use of ABI PRISM 310 sequenator, which enabled to diagnose microsatellite DNA instability. Between 90 and 360 days from the first colonoscopy, 86 (78.2%) patients underwent following endoscopic colonoscopy in order to search for new polyps of large intestine.Results. 130 polypoid adenomas were removed in 97 patients. Sigmoid colon was the most common location of lesions. Microsatellite DNA instability was diagnosed in 21 (16.6%) polyps, loss of heterozygosity was observed in 25 (19%) polyps. During control colonoscopy performed a year after the initial colonoscopy recurrence of polyps was stated in 15% of patients (7% of women and 15% of men). Microsatellite DNA instability was most commonly diagnosed for loci p53 di and DCC. Microsatellite DNA instability was more common in group of younger people and was related with larger polyps.Conclusions. Analysis of microsatellite DNA instability in polypoid adenomas of large intestine provides further essential information within the scope of studies on transformation of adenomas in malignant adenoma of large intestine.








Physical description


1 - 12 - 2009
25 - 1 - 2010


  • 2 Department of General Surgery, Collegium Medicum Jagiellonian University, Cracow
  • Department of Pathomorfology, Collegium Medicum Jagiellonian University, Cracow
  • 2 Department of General Surgery, Collegium Medicum Jagiellonian University, Cracow
  • 2 Department of General Surgery, Collegium Medicum Jagiellonian University, Cracow
  • 2 Department of General Surgery, Collegium Medicum Jagiellonian University, Cracow
  • Students' Society of Science, 2 Department of General Surgery, Collegium Medicum Jagiellonian University, Cracow


  • Fearon ER, Vogelstein B: A genetic model for colorectal tumorigenesis. Cell 1990; 61(5): 759-67.[Crossref][PubMed]
  • Pox C, Schmiegel W: Colorectal screening in Germany. Z Gastroenterol 2008; 46 (Suppl. 1): S31-S32.[Crossref][PubMed]
  • Reguła J, Rupiński M, Kraszewska E, et al.: Colonoscopy in colorectal-cancer screening for detection of advanced neoplasia. N Engl J Med 2006; 355(18): 1863-72.
  • Atkin NB: Microsatellite instability. Cytogenet Cell Genet 2001; 92(3-4): 177-81.[PubMed][Crossref]
  • Cappell MS: From colonic polyps to colon cancer: pathophysiology, clinical presentation, screening and colonoscopic therapy. Minerva Gastroenterol Dietol 2007; 53(4): 351-73.[PubMed]
  • Shinya H, Wolff WI: Morphology, anatomic distribution and cancer potential of colonic polyps. Ann Surg 1979; 190(6): 679-83.[PubMed][Crossref]
  • Gillespie PE, Chambers TJ, Chan KW, et al.: Colonic adenomas - a colonoscopy survey. Gut 1979: 20(3): 240-45.[Crossref]
  • Banaszkiewicz Z, Jarmocik P, Rydzykowski M, i wsp.: Leczenie raka w polipie jelita grubego - 10- letnie doświadczenie. Valetudinaria - Post Med Klin Wojsk 2004; 9(2): 76-80.
  • Velayos FS, Allen BA, Conrad PG, et al.: Low rate of microsatellite instability in young patients with adenomas: reassessing the Bethesda guidelines. Am J Gastroenterol 2005; 100(5): 1143-49.[Crossref][PubMed]
  • Banaszkiewicz Z, Tojek K, Jawień A: Gruczolaki jelita grubego. Przew Lek 2002; 4: 34-36.
  • Koh DC, Luchtefeld MA, Kim DG, et al.: Microsatellite instability and MLH1 hypermethylation - incidence and significance in colorectal polyps in young patients. Colorectal Dis 2007; 9(6): 521-26.[WoS][Crossref]
  • Trawiński J, Patera J, Stanek-Widera A, i wsp.: Zasady diagnostyki patomorfologicznej polipów jelita grubego. Pol Merkuriusz Lek 2007; 22(131): 454-56.
  • Marshall JB: Polipy jelita grubego - odpowiedzi na najważniejsze pytania. Med Dypl 1993; 2(2): 26-32.
  • van Stolk RU, Beck GJ, Baron JA, et al.: Adenoma characteristics at first colonoscopy as predictors of adenoma recurrence and characteristics at follow-up. The Polyp Prevention Study Group. Gastroenterology 1998; 115(1): 13-18.[Crossref]
  • Rubio CA, Nesi G, Messerini L, et al.: The Vienna classification applied to colorectal adenomas. J Gastroenterol Hepatol 2006; 21(11): 1697-1703.[Crossref]
  • Ricciardiello L, Goel A, Mantovani V, et al.: Frequent loss of hMLH1 by promoter hypermethylation leads to microsatellite instability in adenomatous polyps of patients with a single first-degree member affected by colon cancer. Cancer Res 2003; 63(4): 787-92.
  • Samowitz WS, Slattery ML: Transforming growth factor-beta receptor type 2 mutations and microsatellite instability in sporadic colorectal adenomas and carcinomas. Am J Pathol 1997; 151(1): 33-35.
  • Aaltonen LA, Peltomaki P, Mecklin JP, et al.: Replication errors in benign and malignant tumors from hereditary nonpolyposis colorectal cancer patients. Cancer Res 1994; 54(7): 1645-48.[PubMed]
  • Sasaki S, Masaki T, Umetani N, et al.: Microsatellite instability is associated with the macroscopic configuration of neoplasms in patients with multiple colorectal adenomas. Jpn J Clin Oncol 1998; 28(7): 427-30.[Crossref][PubMed]
  • Iino H, Jass JR, Simms LA, et al.: DNA microsatellite instability in hyperplastic polyps, serrated adenomas, and mixed polyps: a mild mutator pathway for colorectal cancer? J Clin Pathol 1999; 52(1): 5-9.[PubMed][Crossref]
  • Olewiński R, Bednarz W, Ogorzałek A: Analiza ploidii DNA w leczonych operacyjnie nowotworach jelita grubego. Pol Przegl Chir 2005; 8(77): 831-44.

Document Type

Publication order reference


YADDA identifier

JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.