Jun Gene Expression is Decreased in Parathyroid Adenoma

• Authors: Jadwiga Żebracka-Gala , Janusz Waler , Jacek Gawrychowski , Kornelia Hasse-Lazar , Małgorzata Kowalska , Andrzej Niemiec , Sylwia Szpak-Ulczok , Beata Jurecka-Lubieniecka , Grzegorz Buła , Witold Truchanowski , Elżbieta Gubała , Barbara Jarząb
• Languages of publication: EN

Abstracts

EN

The aim of the study was to analyze the gene expression of JUN and CCND1 in a group of parathyroid tissues obtained from patients with primary hyperparathyroidism in comparison to hyperplastic parathyroid and normal/atrophic parathyroid tissues by real-time quantitative PCR. Our goal was to validate the conclusion of Forsberg et al (2005) who reported overexpression of JUN in parathyroid adenomas by a joint microarray and QPCR study.Material and methods. The analysis of JUN, CCND1 was carried out by QPCR in 14 parathyroid adenomas, 8 hyperplasia cases and 50 normal/atrophic parathyroid samples taken intraoperatively. Expression of the examined genes was normalized to the reference index (geometric mean of reference genes expression: EIF3S10, UBE2D2, ATP6V1E).Results. We observed a decrease of JUN expression in parathyroid adenomas in comparison to both normal/atrophic and hyperplastic parathyroids. The fold change value was 0.71 in comparison of adenomas to normal/atrophic samples (p = 0.044) and 0.75 to hyperplastic glands (p = 0.003). For CCND1 we observed one case of parathyroid adenoma with a very clearly increased expression, while 3 adenomas (21.4% of all adenomas) exhibited the increase over the highest value seen in normal parathyroids (fold change = 3.52).Conclusions. In parathyroid adenomas we were not able to confirm any overexpression of JUN gene, as suggested by the previous study. On the contrary, we observed a distinct inhibition of JUN RNA expression in comparison to non-neoplastic parathyroids. For CCND1 gene overexpression in parathyroid adenomas, we report the frequency of 21.4%.

Keywords

EN

parathyroid adenoma; JUN gene; CCND1 gene; QPCR

• Publisher: Index Copernicus International
• Journal: Polish Journal of Surgery
• Year: 2009
• Volume: 81
• Issue: 10
• Pages: 457-464

Dates

published

1 - 10 - 2009

online

25 - 11 - 2009

Contributors

author

Jadwiga Żebracka-Gala

• Department of Nuclear Medicine and Endocrine Oncology, M. Skłodowska-Curie MSC Cancer Center and Institute of Oncology, Gliwice

author

Janusz Waler

• Department of General Surgery, Medical University of Silesia, Katowice

author

Jacek Gawrychowski

• Department of General Surgery, Medical University of Silesia, Katowice

author

Kornelia Hasse-Lazar

• Department of Nuclear Medicine and Endocrine Oncology, M. Skłodowska-Curie MSC Cancer Center and Institute of Oncology, Gliwice

author

Małgorzata Kowalska

• Department of Nuclear Medicine and Endocrine Oncology, M. Skłodowska-Curie MSC Cancer Center and Institute of Oncology, Gliwice

author

Andrzej Niemiec

• Department of General Surgery, Medical University of Silesia, Katowice

author

Sylwia Szpak-Ulczok

• Department of Nuclear Medicine and Endocrine Oncology, M. Skłodowska-Curie MSC Cancer Center and Institute of Oncology, Gliwice

author

Beata Jurecka-Lubieniecka

• Department of Nuclear Medicine and Endocrine Oncology, M. Skłodowska-Curie MSC Cancer Center and Institute of Oncology, Gliwice

author

Grzegorz Buła

• Department of General Surgery, Medical University of Silesia, Katowice

author

Witold Truchanowski

• Department of General Surgery, Medical University of Silesia, Katowice

author

Elżbieta Gubała

• Department of Nuclear Medicine and Endocrine Oncology, M. Skłodowska-Curie MSC Cancer Center and Institute of Oncology, Gliwice

author

Barbara Jarząb

• Department of Nuclear Medicine and Endocrine Oncology, M. Skłodowska-Curie MSC Cancer Center and Institute of Oncology, Gliwice

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Identifiers

DOI

10.2478/v10035-009-0075-0