DNA Ploidy in the Treatment of Stomach Adenocarcinoma: A Follow-Up Study

• Authors: Jacek Gawrychowski , Andrzej Tyczyński , Andrzej Danik , Bożena Lackowska , Henryk Koziołek , Ryszard Mucha
• Languages of publication: EN

Abstracts

EN

The aim of the study was to determine unfavourable prognostic factors for patients with stomach adenocarcinoma after their surgical treatment through evaluation of prognostic indicators for cellular ploidy and proliferative activity.Material and methods. A group of 51 patients following surgical treatment due to stomach adenocarcinoma and discharged from our hospital between 1995-2000 were studied here. The material examined was encased in paraffin blocks. After histopathological verification, the tumour segments within these blocks were used for cytofluorometric analysis of DNA-ploidy according to Hedley's method.Results. In 41 (80.4%) stomach cancer patients (out of the total group of 51 patients), diploid tumours were found. No significant relationship between DNA ploidy and sex, age, symptoms, inherited susceptibility to a disease, tumour magnitude, grade of histological malignancy, or grade of clinical progression was observed. Generally, after the stomach cancer operation, the probability of five-year survival was 22.1% for patients with diploid tumours (DI=1.0), and 20% for the patients with aneuploid tumours (DI ± 1.0). The probability of 10-year survival was 19.2% for diploid tumours, and 10% for aneuploid tumours. These differences were not statistically significant (p=0.255).Conclusions. 1. Classical clinicopathologic factors are still the best prognostic criteria for the evaluation of long-term results of surgical treatment for stomach cancer patients. 2. Determination of DNA-ploidy and proliferative activity of stomach cancer did not decrease the gap between long-term results prognosis for surgical treatment and actual results.

Keywords

EN

stomach adenocarcinoma; DNA-ploidy; proliferative activity of stomach adenocarcinoma

• Publisher: Index Copernicus International
• Journal: Polish Journal of Surgery
• Year: 2007
• Volume: 79
• Issue: 12
• Pages: 753-761

Dates

published

1 - 12 - 2007

online

11 - 2 - 2008

Contributors

author

Jacek Gawrychowski

• Department of General Surgery, Silesian Medical University, Katowice

author

Andrzej Tyczyński

• Department of Surgery, Public Hospital, Zabrze

author

Andrzej Danik

• Department of Surgery, Public Hospital, Zabrze

author

Bożena Lackowska

• Department of Neoplasm Pathology, Cracow Branch of Oncology Centre

author

Henryk Koziołek

• Department of General Surgery, Silesian Medical University, Katowice

author

Ryszard Mucha

• Department of General Surgery, Silesian Medical University, Katowice

References

• Kodama Y, Inokuchi K, Soejima K et al.: Growth patterns and prognosis in early gastric carcinoma. Cancer 1983; 51: 320-26.[PubMed][Crossref]
• Nanus DM, Kelsen DP, Niedzwiecki D et al.: Flow cytometry as a predictive indicator in patients with operable gastric cancer. J Clin Oncol 1989; 7: 1105-12.[PubMed]
• Lee KH, Lee JS, Lee JH et al.: Prognostic value of DNA flow cytometry in stomach cancer: a 5-year prospective study; Br J Cancer 1999; 79: 1727-35.[Crossref]
• Hedley DW, Friedlander ML, Taylor IW et al.: Metod for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 1983; 31: 1333-35.[Crossref]
• Shankey TV, Rabinovitch PS, Bagwell B et al.: Guidelines for implementation of clinical DNA cytometry: Cytometry 1993; 14: 472-77.[PubMed][Crossref]
• Sakusabe M, Kodama M, Sato Y et al.: Clinical significance of DNA Ploidy Pattern in stage III Gastric Cancer. World J Surg 1996; 20: 27-31.[PubMed][Crossref]
• Wu YF, Xu HM, Chen JQ: Relations of proliferative activities of gastric carcinoma cells to lymphatic involvement, venous invasion and prognosis. Chin Med J 2004; 117: 1530-35.
• Kim JM, Lee DK, Kim YK et al.: DNA Analysis by Flow Cytometry in Early Gastric Cancer. Korean J Intern Med 1997; 12: 137-43.[PubMed]
• Kimura H, Yonemura Y: Flow cytometric analysis of nuclear DNA content in advanced gastric cancer and its relationship with prognosis. Cancer 1991; 67: 2588-93.
• Rugge M, Sonego F, Panozzo M et al.: Pathology and ploidy in the prognosis of gastric cancer with no extranodal metastastasis. Cancer 1994; 73: 1127-33.[Crossref]
• Lee KH, Lee JS, Suh C et al.: DNA flow cytometry of stomach cancer. Prospective correlation with clinicopathologic findings. Cancer 1993; 72: 1819-26.
• Flyger HL, Christensen IJ, Thorup J et al.: DNA aneuploidy in gastric carcinoma. Flow cytometric data related to survival, location, and histopathologic findings. Scand J Gastroenterol 1995; 30: 258-64.[Crossref][PubMed]
• Bendardaf R, Lamlum H, Ristamaki R et al.: Response to Chemotherapy (Irinotecan plus 5-Fluorouracil) in Colorectal Carcinoma Can Be Predicted by Tumour DNA Content. Oncology 2004; 66: 46-52.[PubMed][Crossref]
• Lee JH, Noh SH, Lee KY et al.: DNA Ploidy Patterns in Advanced Gastric Carcinoma; is it a Clinically Applicable Prognosticator? Hepato-Gastroenterology 2001; 48: 1793-96.
• Esteban F, Vega DS, Garcia R et al.: DNA Content by Flow Cytometry in Gasric Carcinoma: Pathology, Ploidy and Prognosis: Hepato-Gastroenterology 1999; 46: 2039-43.
• Mizunuma H, Tsuchiga A, Ando Y et al.: Flow Cytometric DNA Analysis of Gastric Cancer that is Invading the Muscularis Propria. Eur J Surg 1996; 162: 889-94.
• Yonemura Y, Kimura H, Fushida S et al.: Analysis of proliferative activity using anti-proliferating cell nuclear antigen antibody in gastric cancer tissue specimens obtained by endoscopic biopsy. Cancer 1993; 71: 2448-53.
• Lee KH, Lee JH, Kim TW et al.: A Prospective Correlation of Lauren's Histological of Stomach Cancer with Clinicopathological Findings Including DNA Flow Cytometry: Pathol Res Pract 2001; 197: 223-29.
• Sasaki K, Takahashi M, Hashimoto T et al.: Flow cytometric DNA measurement of gastric cancers. Clinico-pathological implication of DNA ploidy. Path Res Pract 1989; 184: 561-66.
• Ballantyne KC, James PD, Robins RA et al.: Flow cytomeric analysis of the DNA content of gastric cancer. Br J Cancer 1987; 56: 52-54.[Crossref]
• Frierson HF: Flow Cytometric Analysis of Ploidy in Solid Neoplasms; Comparison of Fresh Tissue with Formalin - fixed paraffin - embendend specimens. Hum Pathol 1988; 19: 290-94.[Crossref][PubMed]
• Bauer KD, Bagwell CB, Giaretti W: Consensus review of the clinical utility of DNA flow cytometry in colorectal cancer. Cytometry 1993; 14: 486-91.[Crossref][PubMed]
• Hirose K, Iida A, Yamaguchi A et al.: Prognostic value of DNA Ploidy and Proliferating Cell Nuclear Antygen In Gastric Cancer: Oncology 1998; 55: 300-06.[Crossref]
• Johnoson Jr H, Belluco C, Masood S et al.: The value of flow cytometric analysis in patients with gastric cancer. Arch Surg 1993; 128: 314-17.
• Imada T, Yamamoto Y, Fukuzawa K et al.: Flow Cytometric Analysis of Nuclear DNA Heterogenity in Gastric Cancer: Jap J Clinic Oncol Pages 221-226: 1998.
• Matsumoto T, Yonemura Y, Tsugava K et al.: Study of DNA ploidy heterogeneity and prognosis of gastric cancer primary lesion by FCM. Flow Cytometry 1990; 10: 74-78.
• Sasaki K, Hashimoto T, Kawachino K et al.: Intratumoral regional differences in DNA ploidy of gastrointestinal carcinomas. Cancer 1988; 62: 2569-75.[PubMed][Crossref]
• Haraguchi M, Okamura T, Korenaga D et al.: Heterogenity of DNA ploidy in patients with undiferentiated carcinoma of the stomach. Cancer 1987; 59: 922-24.
• Filipe MI, Rosa J, Sandey A et al.: Is DNA ploidy and proliferative activity of prognostic value in advanced gastric carcinoma? Human Pathol 1991; 22: 373-78.[Crossref]
• Ohyama S, Yonemura Y, Miyzaki I: Proliferative activity and malignancy in human gastric cancers. Cancer 1992; 69: 314-21.

Identifiers

DOI

10.2478/v10035-007-0117-4