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2007 | 79 | 4 | 312-318
Article title

Role of T-lymphocytes in Radiation-Impaired Wound Healing

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Radiation impairs healing, although the underlying mechanisms are not clearly defined. T-lymphocytes have been shown to be critical for wound healing. We hypothesized that radiation-impaired healing may affect different subtypes of T cells.The aim of the study. We studied the effect of local electron irradiation on standard parameters of dermal wound healing in rats and correlated the outcome of healing with the expression of different lymphocyte subtypes in the wound.Material and methods. Groups of 10 rats were irradiated using single dose 12 or 24 Gray electron radiation at the dorsal skin. Control rats were sham-irradiated. On day 5, a skin incision in the irradiated area was performed and polyvinyl alcohol sponges were inserted subcutaneously. Rats were sacrificed 10 days later to determine the wound breaking strength and reparative collagen deposition. Blood lymphocytes were analyzed by FACS. Immunohistochemistry was performed on wound sections.Results. Irradiation significantly reduced wound collagen deposition and wound breaking strength (p <0.05), leading to a 78% reduction in collagen deposition and 47% reduction in breaking strength in 24 Gy animals. Blood lymphocytes were not affected by electron-irradiation, suggesting that the wound was not affected by radiation-induced systemic effects. Impaired healing was reflected, however, in increased expression of wound CD8 cells and decreased expression of CD25 (IL-2 receptor) (p <0.01). No effect was seen on wound CD4 cells. In addition, the ratio of CD4/CD8 was significantly decreased (p <0.05).Conclusions. Radiation-impaired healing is reflected in impaired expression of wound lymphocyte subtypes. Altered lymphocyte subtypes may affect the outcome of healing in irradiated wounds.

Physical description
1 - 4 - 2007
17 - 10 - 2007
  • Department of Surgery, University Hospital, Knappschaftskrankenhaus Bochum-Langendreer, Germany
  • Department of Surgery, University Hospital, Knappschaftskrankenhaus Bochum-Langendreer, Germany
  • Department of Surgery, University Hospital, Knappschaftskrankenhaus Bochum-Langendreer, Germany
  • Schäffer M, Barbul A: Lymphocyte function in wound healing and following injury. Br J Surg 1998; 85: 444-60.
  • Efron JE, Frankel HL, Lazarou SA et al.: Wound healing and T-lymphocytes. J Surg Res 1990; 48: 460-63.
  • Barbul A, Breslin RJ, Woodyard JP et al.: The effect of in vivo T helper and T suppressor lymphocyte depletion on wound healing. Ann Surg 1989; 209: 479-83.
  • Tibbs MK: Wound healing following radiation therapy: a review. Radiother Oncol 1997; 42: 99.
  • Bernstein EF, Sullivan FJ, Mitchell JB et al.: Biology of chronic radiation effect on tissues and wound healing. Clin Plast Surg 1993; 20: 435.
  • Fowler JF, Kragt K, Ellis R: The effect of divided doses of 15 MeV electrons on skin response of mice. Int J Radiat Biol 1965; 9: 241.
  • Morris GM, Hopewell JW. Change in the cell kinetics of pig epidermis after single dose of X-rays. Br J Radiol 1988; 61: 205.
  • Schäffer M, Bongartz M, Hoffmann W et al.: Regulation of Nitric Oxide Synthesis in Wounds by IFN-γ depends on TNF-α. J Invest Surg 2006; 19: 371-79.
  • Woessner J: The determination of hydroxyproline in tissue and protein samples containing small proportions of this amino acid. Arch Biochem Biophys 1961; 93: 440-47.
  • Bujko K, Suit HD, Springfield DS et al.: Wound healing after preoperative radiation for sarcoma of soft tissues. Surg Gynecol Obstet 1993; 176: 124-34.
  • Gorodetsky R, McBride WH, Withers HR: Assay of radiation effects in mouse skin as expressed in wound healing. Radiat Res 1988; 166: 135-44.
  • Wang Q, Dickson GR, Abraham WP et al.: Electron irradiation slows down wound repair in rat skin: a morphological investigation. Br J Dermatol 1994; 130: 551-60.
  • Vegesna V, McBride WH, Withers HR: Postoperative irradiation impairs or enhances wound strength depending on time of administration. Radiat Res 1995; 143: 224-28.
  • Gabka CJ, Benhain P, Mathes SJ et al.: An experimental model to determine the effect of irradiated tissue on neutrophil function. Plast Reconstr Surg 1995; 96: 1676-88.
  • Bernstein EF, Salomon GD, Harisiadis L et al.: Collagen gene expression and wound strength in normal and radiation-impaired wounds. A model of radiation-impaired wound healing. J Dermatol Surg Oncol 1993; 19: 564-70.
  • Yanase A, Ueda M, Kaneda T et al.: Irradiation effects on wound contraction using a connective tissue model. Ann Plast Surg 1993; 30: 435-40.
  • Barbul A, Shaw T, Rotter SM et al.: Wound healing in nude mice: A study on the regulatory role of lymphocytes in fibroplasia. Surgery 1989; 105: 764-69.
  • Davis PA, Corless DJ, Aspinall R et al.: Effect of CD4+ and CD8+ cell depletion on wound healing. Br J Surg 2001; 88: 298-304.
  • Chircop MP, Yu Y, Berney CR et al.: Wound healing and growth factor expression in T lymphocyte deficiency. ANZ J Surg 2002; 72: 491-95.
  • Barbul A, Shawe T, Frankel HL et al.: Inhibition of wound repair by thymic hormones. Surgery 1989; 106: 373-76.
  • Prudden JF, Nishihara G, Ocamp I: Studies on growth hormone: III. The effect on wound tensile strength of marked postoperative anabolism induced with growth hormone. Surg Gynecol Obstet 1958; 107: 481-82.
  • Ehrlich HP, Hunt TK: The effects of cortisone and anabolic steroids on the tensile strength of healing wounds. Ann Surg 1969; 170: 203-06.
  • Witte MB, Barbul A: Arginine physiology and its implication for wound healing. Wound Repair Regen 2003; 11: 419-23.
  • Fishel R, Barbul A, Wasserkrug HL et al.: Cyclosporin A impairs wound healing in rats. J Surg Res 1983; 34: 572-75.
  • Schäffer M, Fuchs N, Völker J et al.: Differential effect of tacrolimus on dermal and intestinal healing. J Invest Surg 2005; 18: 71-79.
  • Barbul A, Sisto D, Rettura G et al.: Thymic inhibition of wound healing: abrogation by adult thymectomy. J Surg Res 1982; 32: 338-42.
  • Jameson J, Ugarte K, Chen N et al.: A role for skin gammadelta T cells in wound repair. Science 2002; 296: 747-49.
  • Boyce DE, Jones WD, Ruge F et al.: The role of lymphocytes in human dermal wound healing. Br J Dermatol 2000; 143: 59-65.
  • Parks JE, Barbul A: Understanding the role of immune regulation in wound healing. Am J Surg 2004; 187: 11-16.
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