Immobilization of Aspergillus oryzae β galactosidase on concanavalin A-layered calcium alginate-cellulose beads and its application in lactose hydrolysis in continuous spiral bed reactors

• Authors: Shakeel Ansari , Qayyum Husain
• Languages of publication: EN

Abstracts

EN

In this study, Aspergillus oryzae β galactosidase was immobilized on concanavalin A layered calcium alginate-cellulose beads as a bioaffinity support. Immobilized enzyme showed a remarkable broadening in temperature-activity profiles as compared to the native enzyme and exhibited 65% activity in the presence of 5% galactose. Michaelis constant (Km) was 2.57 mM and 5.38 mM for the free and the immobilized β galactosidase, respectively. Crosslinked β galactosidase showed greater catalytic activity in the presence of Mg2+ and was more stable during storage at 4°C for 6 weeks. Immobilized enzyme hydrolyzed 67% lactose in milk in 8 h and 85% lactose in whey in 9 h in the stirred batch process at 50°C. The continuous hydrolysis of lactose by crosslinked β galactosidase in spiral bed reactor exhibited 93% and 88% hydrolysis of lactose at flow rate of 20 ml/h and 30 ml/h, after 1 month operation, respectively.

Keywords

EN

β galactosidase; Immobilization; Cellulose-alginate; Concanavalin A; Spiral bed reactor; Lactose hydrolysis

• Publisher: De Gruyter Open
• Journal: Polish Journal of Chemical Technology
• Year: 2011
• Volume: 13
• Issue: 4
• Pages: 15-20

Dates

published

1 - 1 - 2011

online

2 - 1 - 2012

Contributors

author

Shakeel Ansari

• Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, India

author

Qayyum Husain

• Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, India

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Identifiers

DOI

10.2478/v10026-011-0043-4