Cytokine production by leukocytes in patients with periodontitis

• Authors: Jurgina Sakalauskiene , Dalia Giedrimiene , Ricardas Kubilius , Alvydas Gleiznys , Astra Vitkauskiene , Egle Ivanauskiene , Kristina Puskunigyte
• Languages of publication: EN

Abstracts

EN

In the present study, we investigated the relation between clinical parameters and levels of interleukin (IL) -4 and -5, and tumour necrosis factor-α (TNF-α) in the leukocyte incubation medium (LIM) obtained from 26 patients with chronic periodontitis (P) and 26 control group subjects (C). The levels of cytokines IL-4 and IL -5 produced by the LIM stimulated with non-opsonised E. coli were determined using the Enzyme-Linked Immunosorbent Assay (ELISA) method and the levels of TNF-α were evaluated by applying Enzyme Amplified Sensitivity Immunoassay (EASIA). TNF-α levels in stimulated LIM were strongly positively correlated with clinical parameters such as the pocket probing depths (PPD), the clinical attachment level (CAL), the bleeding on probing (BOP) and oral hygiene index (OHI), whereas the IL-4 and IL-5 levels in the analogous medium were strongly negatively correlated with the clinical parameters. IL-4 and IL-5 levels in stimulated LIM of P group patients were significantly lower, whereas TNF-α levels were significantly higher than that in analogous medium of C group subjects. These differences were associated with the severity of periodontal disease.

Keywords

EN

Periodontitis; Interleukin 4, 5; Tumor necrosis factor α

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2014
• Volume: 9
• Issue: 6
• Pages: 821-829

Dates

published

1 - 12 - 2014

online

16 - 8 - 2014

Contributors

author

Jurgina Sakalauskiene

• Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania

author

Dalia Giedrimiene

• School of Health and Natural Sciences & School of Pharmacy, University of Saint Joseph, Saint Joseph, CT., 06117, USA

author

Ricardas Kubilius

• Department of Maxillofacial Surgery and Surgical Stomatology, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania

author

Alvydas Gleiznys

• Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania

author

Astra Vitkauskiene

• Department of Laboratory Medicine, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania

author

Egle Ivanauskiene

• Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania

author

Kristina Puskunigyte

• Clinic Stomatology Odenta, LT-50106, Kaunas, Lithuania

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Identifiers

DOI

10.2478/s11536-014-0699-2