PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2014 | 9 | 6 | 821-829
Article title

Cytokine production by leukocytes in patients with periodontitis

Content
Title variants
Languages of publication
EN
Abstracts
EN
In the present study, we investigated the relation between clinical parameters and levels of interleukin (IL) -4 and -5, and tumour necrosis factor-α (TNF-α) in the leukocyte incubation medium (LIM) obtained from 26 patients with chronic periodontitis (P) and 26 control group subjects (C). The levels of cytokines IL-4 and IL -5 produced by the LIM stimulated with non-opsonised E. coli were determined using the Enzyme-Linked Immunosorbent Assay (ELISA) method and the levels of TNF-α were evaluated by applying Enzyme Amplified Sensitivity Immunoassay (EASIA). TNF-α levels in stimulated LIM were strongly positively correlated with clinical parameters such as the pocket probing depths (PPD), the clinical attachment level (CAL), the bleeding on probing (BOP) and oral hygiene index (OHI), whereas the IL-4 and IL-5 levels in the analogous medium were strongly negatively correlated with the clinical parameters. IL-4 and IL-5 levels in stimulated LIM of P group patients were significantly lower, whereas TNF-α levels were significantly higher than that in analogous medium of C group subjects. These differences were associated with the severity of periodontal disease.
Publisher

Journal
Year
Volume
9
Issue
6
Pages
821-829
Physical description
Dates
published
1 - 12 - 2014
online
16 - 8 - 2014
Contributors
  • Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania, jur.sak12@gmail.com
  • School of Health and Natural Sciences & School of Pharmacy, University of Saint Joseph, Saint Joseph, CT., 06117, USA
  • Department of Maxillofacial Surgery and Surgical Stomatology, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania
  • Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania
  • Department of Laboratory Medicine, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania
  • Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, LT-50106, Kaunas, Lithuania
  • Clinic Stomatology Odenta, LT-50106, Kaunas, Lithuania
References
  • [1] Paster BJ, Olsen I, Aas JA, Dewhirst FE. The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol 2000. 2006;42:80–87 http://dx.doi.org/10.1111/j.1600-0757.2006.00174.x[Crossref]
  • [2] Ebersole JE. Humoral immune response in gingival crevice fluid: local and systemic implications. Periodontol 2000. 2003;31:135–166 http://dx.doi.org/10.1034/j.1600-0757.2003.03109.x[Crossref]
  • [3] Holt SC, Ebersole JL. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the „red complex“, a prototype polybacterial pathogenic consortium in periodontitis. Periodontol 2000. 2005;38:72–122 http://dx.doi.org/10.1111/j.1600-0757.2005.00113.x[Crossref]
  • [4] Bodet C, Chandad F, Grenier D. Pathogenic potential of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia, the red bacterial complex associated with periodontitis. Pathol Biol (Paris). 2007;55(3–4):154–162 http://dx.doi.org/10.1016/j.patbio.2006.07.045[WoS][Crossref]
  • [5] Graves DT, Cochran D. The contribution of interleukin-1 and tumor necrosis factor to periodontal tissue destruction. J Periodontol. 2003;74(3):391–401 http://dx.doi.org/10.1902/jop.2003.74.3.391[Crossref]
  • [6] Page RC, Kornman KS. Pathogenesis of human periodontitis: an introduction. Periodontol 2000. 1997;14:9–11 http://dx.doi.org/10.1111/j.1600-0757.1997.tb00189.x[Crossref]
  • [7] Sorensen LK, Havemose-Poulsen A, Bendtzen K, Holmstrup P. Aggressive periodontitis and chronic arthritis blood mononuclear cell gene expression and plasma protein levels o cytokines and cytokine inhibitors. J Periodontol 2009;80(2):282–289. doi: 10.1902/jop.2009.080347 http://dx.doi.org/10.1902/jop.2009.080347[WoS][Crossref]
  • [8] Ishihara Y, Nishihara T, Kuroyanagi T, Shirozu N, Yamagishi E, Ohguchi M, et al. Gingival crevicular interleukin-1 and interleukin-1 receptor antagonist levels in periodontally healthy and diseased sites. J Periodontal Res. 1997;32(6):524–529 http://dx.doi.org/10.1111/j.1600-0765.1997.tb00568.x[Crossref]
  • [9] Gorska R, Gregorek H, Kowalski J, Laskus-Perendyk A, Syczewska M, Madalinski K. Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis. J Clin Periodontol. 2003;30(2):1046–1052 http://dx.doi.org/10.1046/j.0303-6979.2003.00425.x[Crossref]
  • [10] Belardelli F, Ferrantini M. Cytokines as a link between innate and adaptive antitumor immunity. Trends Immunol. 2002;23(4):201–208 http://dx.doi.org/10.1016/S1471-4906(02)02195-6[Crossref]
  • [11] Kinane DF, Preshaw PM, Loos BG. Host-response: understanding the cellular and molecular mechanisms of host-microbial interactions-consensus of the Seventh European Workshop on Periodontology. J Clin Periodontol. 2011;38Suppl 11:44–48. doi: 10.1111/j.1600-051X.2010.01682.x http://dx.doi.org/10.1111/j.1600-051X.2010.01682.x[WoS][Crossref]
  • [12] Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol. 2011;38Suppl 11:60–84. doi: 10.1111/j.1600-051X.2010.01671.x http://dx.doi.org/10.1111/j.1600-051X.2010.01671.x[WoS][Crossref]
  • [13] O’Brien-Simpson NM, Pathirana RD, Walker GD, Reynolds EC. Porphyromonas gingivalis RgpA-Kgp proteinase-adhesin complexes penetrate gingival tissue and induce proinflammatory cytokines or apoptosis in a concentration-dependent manner. Infect Immun. 2009;77(3):1246–1261. doi: 10.1128/IAI.01038-08 http://dx.doi.org/10.1128/IAI.01038-08[Crossref][WoS]
  • [14] Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999;4:1–6 http://dx.doi.org/10.1902/annals.1999.4.1.1[Crossref]
  • [15] Green JC, Vermillion JR. The simplified oral hygiene index. J Am Dent Assoc. 1964;68:7–13 [Crossref]
  • [16] Timm M, Baehr R, Hildebrand A. [The lysozyme liberation test - a method for the demonstration of stimulation of human neutrophil granulocytes and monocytes]. Allerg Immunol (Leipz). 1984;30(3):175–182. German
  • [17] Hutter G, Schlagenhauf U, Valenza G, Horn M, Burgemeister S, Claus H, et al. Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens. Microbiology. 2003;149(Pt 1):67–75 http://dx.doi.org/10.1099/mic.0.25791-0[Crossref]
  • [18] Graves DT, Fine D, Teng YT, Van Dyke TE, Hajishengallis G. The use of rodent models to investigate host-bacteria interactions related to periodontal diseases. J Clin Periodontol. 2008 35(2):89–105. doi: 10.1111/j.1600-051X.2007.01172.x http://dx.doi.org/10.1111/j.1600-051X.2007.01172.x[Crossref][WoS]
  • [19] Liu YC, Lerner UH, Teng YT. Cytokine responses against periodontal infection: protective and destructive roles. Periodontol 2000. 2010;52(1):163–206. doi: 10.1111/j.1600-0757.2009.00321.x http://dx.doi.org/10.1111/j.1600-0757.2009.00321.x[WoS][Crossref]
  • [20] Mahanonda R, Pichyangkul S. Toll-like receptors and their role in periodontal health and disease. Periodontol 2000. 2007;43:41–55 http://dx.doi.org/10.1111/j.1600-0757.2006.00179.x[Crossref]
  • [21] Kikkert R, Laine ML, Aarden LA, van Winkelhoff AJ. Activation of Toll-like receptors 2 and 4 by Gramnegative periodontal bacteria. Oral Microbiol Immunol. 2007;22(3):145–151 http://dx.doi.org/10.1111/j.1399-302X.2007.00335.x[Crossref][WoS]
  • [22] Lima HR, Gelani V, Fernandes AP, Gasparoto TH, Torres SA, Santos CF, et al. The essential role of toll like receptor-4 in the control of Aggregatibacter actinomycetemcomitans infection in mice. J Clin Periodontol. 2010;37(3):248–254 http://dx.doi.org/10.1111/j.1600-051X.2009.01531.x[Crossref]
  • [23] Bodet C, Grenier D. Synergistic effects of lipopolysaccharides from periodontopathic bacteria on proinflammatory cytokine production in an ex vivo whole blood model. Mol Oral Microbiol. 2010;25(2):102–111 doi: 10.1111/j.2041-1014.2010.00566.x [Crossref][WoS]
  • [24] Andrukhov O, Ulm C, Reischl H, Nguyen PQ, Matejka M, Rausch-Fan X. Serum cytokine levels in periodontitis patients in relation to the bacterial load. J Periodontol. 2011;82(6):885–892. doi: 10.1902/jop.2010.100425 http://dx.doi.org/10.1902/jop.2010.100425[Crossref][WoS]
  • [25] Jurisic V, Terzic T, Colic S, Jurisic M. The concentration of TNF-α correlate with number of in-flammatory cells and degree of vascularization in radicular cysts. Oral Dis. 2008;14(7):600–605. doi: 10.1111/j.1601-0825.2007.01426.x http://dx.doi.org/10.1111/j.1601-0825.2007.01426.x[Crossref][WoS]
  • [26] Chen YW, Umeda M, Nagasawa T, Takeuchi Y, Huang Y, Inoue Y, et al. Periodontitis may increase the risk of peripheral arterial disease. Eur J Vasc Endovasc Surg. 2008;35(2):153–158 http://dx.doi.org/10.1016/j.ejvs.2007.08.016[Crossref][WoS]
  • [27] Mochizuki S, Sakurai K. Dectin-1 targeting delivery of TNF-α antisense ODNs complexed with β-1,3-glucan protects mice from LPS-induced hepatitis. J Control Release 2011;151(2):155–161. doi: 10.1016/j.jconrel.2011.01.026 http://dx.doi.org/10.1016/j.jconrel.2011.01.026[WoS][Crossref]
  • [28] Zhu J, Paul WE. Peripheral CD4+ T-cell differentiation regulated by networks of cytokines and transcription factors. Immunol Rev. 2010;238(1):247–262. doi: 10.1111/j.1600-065X.2010.00951.x http://dx.doi.org/10.1111/j.1600-065X.2010.00951.x[WoS][Crossref]
  • [29] Inagaki A, Ishida T, Ishii T, Komatsu H, Iida S, Ding J, et al. Clinical significance of serum Th1-, Th2- and regulatory T cells-associated cytokines in adult T-cell leukemia/lymphoma: high interleukin-5 and -10 levels are significant unfavorable prognostic factors. Int J Cancer 2006;15;118(12):3054–3061 http://dx.doi.org/10.1002/ijc.21688[Crossref]
  • [30] Pradeep AR, Roopa Y, Swati PP. Interleukin-4, a T-helper 2 cell cytokine, is associated with the remission of periodontal disease. J Periodontal Res. 2008;43(6):712–716. doi: 10.1111/j.1600-0765.2007.01079.x http://dx.doi.org/10.1111/j.1600-0765.2007.01079.x[WoS][Crossref]
  • [31] Robati M, Ranjbari A, Ghafourian Boroujerdnia M, Chinipardaz Z. Detection of IL-4, IL-6 and IL-12 serum levels in generalized aggressive periodontitis. Iran J Immunol. 2011;8(3):170–175
  • [32] Tam V, O’Brien-Simpson NM, Chen YY, Sanderson CJ, Kinnear B, Reynolds EC. The RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis Inactivate the Th2 cytokines interleukin-4 and interleukin-5. Infect Immunol. 2009;77(4):1451–1458. doi: 10.1128/IAI.01377-08 http://dx.doi.org/10.1128/IAI.01377-08[Crossref]
  • [33] Tobón-Arroyave SI, Jaramillo-González PE, Isaza-Guzmán DM. Correlation between salivary IL-1beta levels and periodontal clinical status. Arch Oral Biol. 2008;53(4):346–352 http://dx.doi.org/10.1016/j.archoralbio.2007.11.005[Crossref][WoS]
  • [34] Ohlrich EJ, Cullinan MP, Seymour GJ. The immunopathogenesis of periodontal disease. Aust Dent J. 2009;54 Suppl 1:S2–10 http://dx.doi.org/10.1111/j.1834-7819.2009.01139.x[Crossref]
  • [35] Mkonyi LE, Bakken V, Sovik JB, Mauland EK, Fristad I, Barczyk MM, Bletsal A, Berggreen E. Lymphangiogenesis is induced during development of periodontal disease. J Dent Res. 2012 Jan;91(1):71–77 http://dx.doi.org/10.1177/0022034511424747[Crossref]
  • [36] Garlet GP. Destructive and protective roles of cytokines in periodontitis: a re-appraisal from host defense and tissue destruction viewpoints. J Dent Res. 2010 Dec;89(12):1349–1363. doi: 10.1177/0022034510376402 http://dx.doi.org/10.1177/0022034510376402[Crossref][WoS]
  • [37] Sonck E, Stuyven E, Goddeeris B, Cox E. The effect of beta-glucans on porcine leukocytes. Vet Immunol Immunopathol. 2010;135(3–4):199–207. doi: 10.1016/j.vetimm.2009.11.014 http://dx.doi.org/10.1016/j.vetimm.2009.11.014[Crossref]
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11536-014-0699-2
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.