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2012 | 7 | 4 | 533-538

Article title

Effective melatonin protection of burn-induced hepatic disorders in rats


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We studied the effect of melatonin on morphological and functional disorders using serum markers of liver dysfunction such as cholinesterase and gamma glutamyl transpeptidase, hepatic protein content and malondialdehyde in a burned-rat model. Melatonin (10 mg/kg (−1), i.p) was administered immediately and then 12 h after 30% of total body surface area burns of male Wistar rats. The burns induced an increase of hepatic malondialdehyde levels by 166% (p<0.001), and also vascular congestion, leukocyte infiltration around the central veins, intracellular vacuolization, hepatic cell degeneration and apoptotic bodies (Councilman’s bodies). These changes were associated with significantly reduced serum cholinesterase (36%), gamma glutamyl transpeptidase (76%), hepatic proteins (52%) and serum albumin (37%) (p<0.001–0.0001). Treatment with melatonin reduced elevated hepatic malondialdehyde values by 50% (p<0.01). Melatonin restricted degenerative alteration in the hepatocytes: it protected the burninduced decrease of serum gamma glutamyl transpeptidase activity by 48% (p<0.01), hepatic proteins by 64% (p<0.01), and serum activity of cholinesterase as the only marker of liver damaged synthetic function by 57% (p<0.0001) but did not exert any significant influence on serum albumin concentration. Melatonin repaired the pathomorphological lesions and functional disorders. It could restore liver damage following thermal injury in humans.










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1 - 8 - 2012
24 - 5 - 2012


  • Department of Pathophysiology, Medical University-Varna, 55 Marin Drinov St., BG-9002, Varna, Bulgaria
  • Department of General and Clinical Pathology, Medical University-Varna, 55 Marin Drinov St., BG-9002, Varna, Bulgaria
  • Department of Clinical Laboratory, Medical University-Varna, 55 Marin Drinov St., BG-9002, Varna, Bulgaria
  • Department of Pathophysiology, Medical University-Varna, 55 Marin Drinov St., BG-9002, Varna, Bulgaria


  • [1] Lausevic Z., Lausevic M., Trbojevic-Stankovic B., Krstic S., Stojimirovic B. Predicting multiple organ failure in patients with severe trauma, Can. J. Surg., 2008, 51, 97–102
  • [2] Jeschke MG. Hepatic response to thermal injury: Is the liver important for postburn outcomes, Mol. Med., 2009, 15, 337–351 http://dx.doi.org/10.2119/molmed.2009.00005[Crossref][WoS]
  • [3] Jeschke M.G. Cytokine expression profile over time in severely burned pediatric patients, Shock, 2006, 26, 13–19
  • [4] Zheng H., Chen X.L., Han Z.X., Wang S.Y, Chen Z.W. Effect of Ligustrazine on liver injury after burn trauma, Burns, 2006, 32, 328–334 http://dx.doi.org/10.1016/j.burns.2005.10.007[Crossref]
  • [5] Tan D.X., Manchester L.C., Hardeland R., Lopez-Burillo S., Mayo J.C., Sainz R.M. Melatonin: a hormone, a tissue factor, an autocoid, a paracoid, and an antioxidant vitamin, J. Pineal Res., 2003, 34, 75–78 http://dx.doi.org/10.1034/j.1600-079X.2003.02111.x[Crossref]
  • [6] Reiter R.J., Tan D.X., Qi W., Manchester L.C., Karbownik M., Calvo J.R., Pharmacology and physiology of melatonin in the reduction of oxidative stress in vivo, Biol. Signals Recept., 2000, 9, 160–171 http://dx.doi.org/10.1159/000014636[Crossref]
  • [7] Reiter R.J., Tan D.X. Melatonin: a novel protective agent against oxidative injury of the ischemic/reperfused heart, Cardiovasc. Res., 2003, 58, 10–19 http://dx.doi.org/10.1016/S0008-6363(02)00827-1[Crossref]
  • [8] Sigala F., Theocharis S., Sigalas K., Markantonis-Kyroudis S., Papalabros E., Triantafyllou A., et al., Therapeutic value of melatonin in an experimental model of liver injury and regeneration, J. Pineal Res., 2006, 40, 270–279 http://dx.doi.org/10.1111/j.1600-079X.2005.00310.x[Crossref]
  • [9] Zhang W.H., Li J.Y., Zhou Y. Melatonin abates liver ischemia/reperfusion injury byimproving the balance between nitric oxide and endothelin, Hepatobiliary Pancreat. Dis. Int., 2006, 5, 574–579
  • [10] Ogeturk M., Kus I., Pekmez H., Yekeler H., Sahin S., Sarsilmaz M. Inhibition of carbon tetrachloride-mediated apoptosis and oxidative stress by melatonin in experimental liver fibrosis, Toxicol. Ind. Health, 2008, 24, 201–208 http://dx.doi.org/10.1177/0748233708093725[Crossref][WoS]
  • [11] Sener G., Sehirli A.O., Satiroğlu H., Keyer-Uysal M., C Yeğen B. Melatonin improves oxidative organ damage in a rat model of thermal injury, Burns, 2002, 28, 419–425 http://dx.doi.org/10.1016/S0305-4179(02)00053-0[Crossref]
  • [12] Bekyarova G., Tancheva S., Hristova M. Protective effect of melatonin against oxidative hepatic injury after experimental thermal trauma, Methods Find. Exp. Clin. Pharmacol., 2009, 31, 11–14. http://dx.doi.org/10.1358/mf.2009.31.1.1346609[Crossref]
  • [13] Porter N.A., Nixon J., Isaac J. Cyclic peroxidase and thiobarbituric assay, Biochim. Biophys. Acta, 1976, 441, 596–599
  • [14] Henderson A.R., Moss D.W. Enzymes. In: Tietz Fundamentals of Clinical Chemistry. 5th ed. Burbs, C.A., Ashwood, E.R. (Eds.), Saunders, Philadelphia, 2001.
  • [15] Song J., Finnerty C.C., Herndon D.N., Boehning D., Jeschke M.G. Severe burn-induced endoplasmic reticulum stress and hepatic damage in mice, Mol. Med., 2009, 15, 316–320 http://dx.doi.org/10.2119/molmed.2009.00048[Crossref]
  • [16] Toklu H.Z., Sener G., Jahovic N., Uslu B., Arbak S., Yegen B.C. Beta-glucan protects against burn-induced oxidative organ damage in rats, Int. Immunopharmacol., 2006, 6, 156–169 http://dx.doi.org/10.1016/j.intimp.2005.07.016[Crossref]
  • [17] Jeschke M.G., Low J.F., Spies M., Vita R., Hawkins H.K., Herndon D.N., et al., Cell proliferation, apoptosis, NF-kappaB expression, enzyme, protein, and weight changes in livers of burned rats, Am. J. Physiol. Gastrointest. Liver Physiol., 2001, 280, G1314–1320
  • [18] Sakarcan A., Sehirli O., Velioglu-Ovünç A., Ercan F., Erkanl G., Gedik N., et al., Ginkgo biloba extract improves oxidative organ damage in a rat model of thermal trauma, J. Burn Care Rehabil., 2005, 26, 515–524 http://dx.doi.org/10.1097/01.bcr.0000185115.17261.50[Crossref]
  • [19] Gravante G., Delogu D., Sconocchia G. “Systemic apoptotic response” after thermal burns, Apoptosis, 2007, 12, 259–270 http://dx.doi.org/10.1007/s10495-006-0621-8[WoS][Crossref]
  • [20] Korkmaz A., Reiter R.J., Topal T., Manchester L.C., Oter S., Tan D.X. Melatonin: an established antioxidant worthy of use in clinical trials, Mol. Med., 2009, 15, 43–50 [WoS]
  • [21] Kim S.H., Lee S.M. Cytoprotective effects of melatonin against necrosis and apoptosis induced by ischemia/reperfusion injury in rat liver, J. Pineal Res., 2008, 44, 165–171 http://dx.doi.org/10.1111/j.1600-079X.2007.00504.x[Crossref]
  • [22] Hu S., Yin S., Jiang X., Huang D., Shen G. Melatonin protects against alcoholic liver injury by attenuating oxidative stress, inflammatory response, and apoptosis, Eur. J. Pharmacol., 2009, 616, 287–292 http://dx.doi.org/10.1016/j.ejphar.2009.06.044[WoS][Crossref]
  • [23] Zhang H., Forman HJ., Choi J. Gamma glutamil transpeptidase in glutathione biosynthesis, Methods Enzymol., 2005, 401, 468–483 http://dx.doi.org/10.1016/S0076-6879(05)01028-1[Crossref][WoS]
  • [24] Agay D., Sandre C., Ducros V., Faure H., Cruz C., Alonso A., et al., Optimization of selenium status by a single intraperitoneal injection of Se in Sedeficient rat: possible application to burned patient treatment, Free Radic. Biol. Med., 2005, 39, 762–768 http://dx.doi.org/10.1016/j.freeradbiomed.2005.04.024[Crossref]
  • [25] Urata Y., Honma S., Goto S., Todoroki S., Iida T., Cho S., et al., Melatonin induces gamma-glutamylcysteine synthetase mediated by activator protein-1 in human vascular endothelial cells, Free Radic. Biol. Med., 1999, 27, 838–847 http://dx.doi.org/10.1016/S0891-5849(99)00131-8[Crossref]
  • [26] Kamolz L.P., Andel H., Greher M., Andel D., Meissl G., Frey M. Serum cholinesterase activity in patients with burns, Clin. Chem. Lab. Med., 2002, 40, 60–64 http://dx.doi.org/10.1515/CCLM.2002.012[Crossref]
  • [27] Marko P., Layon A.J., Caruso L., Mozingo D.W., Gabrielli A. Burn injuries, Curr. Opin. Anaesthesiol., 2003, 16, 183–191 http://dx.doi.org/10.1097/00001503-200304000-00012[Crossref]

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