Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl


Preferences help
enabled [disable] Abstract
Number of results


2011 | 6 | 6 | 696-700

Article title

Changes of platelets’ function in preeclampsia


Title variants

Languages of publication



Increased aggregation of platelets during preeclampsia was shown in several studies, yet several others reported no change. The aim of our study was to investigate platelet aggregation in a group of patients suffering from preeclampsia. In a cross-sectional study blood samples were taken from 89 hospitalized patients in the third trimester of pregnancy: 38 were suffering from mild to moderate preeclampsia and 51 patients were without preeclampsia. From the blood samples platelet aggregation, secretion of adenine nucleotides from platelets, concentration of energy-rich adenine compounds and levels of cyclic adenosine-mono-phosphate and cyclic guanosine mono-phosphate in platelets were measured. In the patients with preeclampsia, the adenosine diphosphate threshold for biphasic aggregation [odds ratio (OR):.75; 95% Confidence Interval (CI): 0.55–1.02; p<0.05], total adenine nucleotides concentration in the metabolic pool of platelets (OR:0.99; CI: 0.62–1.57; p<0.01) and cyclic adenosine-mono-phosphate (OR:0.81; CI: 0.57, 1.14; p<0.05) and cyclic guanosine mono-phosphate (OR:.78; CI: 0.55–1.09; p<0.05) levels in platelets were decreased in comparison with the control group, while adenylate energy charge in the metabolic pool of platelets (OR: >100.00; CI: 0.00->100.00; p<0.05) and secretion of adenosine triphosphate (OR:.13; CI: 0.00–14.26; p<0.05) and adenosine diphosphate (OR:.77; CI: 0.08–36.79; p<0.05) were increased. The results of our study show increased activation and aggregation of platelets in pregnant females with preeclampsia.











Physical description


1 - 12 - 2011
8 - 10 - 2011


  • Clinic for Gynecology and Obstetrics, Clinical Center “Kragujevac”, Zmaj Jovina 30, Serbia, Kragujevac, 34000
  • Department of Pharmacy, Medical Faculty, University of Kragujevac, Svetozara Markovica 69, Serbia, Kragujevac, 34000
  • Department of Clinical Pharmacology, Clinical Center “Kragujevac”, 34000, Zmaj Jovina 30, Serbia, Kragujevac


  • [1] Wagner L.K., Diagnosis and Management of Preeclampsia, Am. Fam. Physician., 2004, 70, 2317–2324
  • [2] Vásárhelyi B., Cseh Á., Kocsis I., Treszl A., Györffy B., Rigó Jr. J., Three mechanisms in the pathogenesis of pre-eclampsia suggested by over-represented transcription factor-binding sites detected with comparative promoter analysis, Mol. Human Rep., 2006, 12, 31–34 http://dx.doi.org/10.1093/molehr/gal001[Crossref]
  • [3] Felfernig-Boehm D., Salat A., Vogl S.E., Murabito M., Felfernig M, Schmidt D., et al., Early Detection of Preeclampsia by Determination of Platelet Aggregability, Thromb. Res., 2000, 98, 139–146 http://dx.doi.org/10.1016/S0049-3848(99)00224-8[Crossref]
  • [4] Granger J.P., Alexander B.T., Llinas M.T., Bennett W.A., Khalil R.A., Pathophysiology of Hypertension During Preeclampsia Linking Placental Ischemia With Endothelial Dysfunction, Hypertens., 2001, 38[part 2], 718–722 [Crossref]
  • [5] Young B.C., Levine R.J., Karumanchi S.A., Pathogenesis of preeclampsia, Annu. Rev. Pathol., 2010, 5, 173–192. http://dx.doi.org/10.1146/annurev-pathol-121808-102149[Crossref]
  • [6] Vladareanu A-M., Andrei C., Onisai M., Vasilache V., Bumbea H., Vladareanu R., The endothelialplatelet dysfunction in preeclampsia, J. Clin. Med., 2007, 2, 214–221
  • [7] Crowther C.A., Hiller J.E., Moss J.R., McPhee A.J., Jeffries W.S., Robinson J.S., Effect of Treatment of Gestational Diabetes Mellitus on Pregnancy Outcomes, N. Engl. J. Med., 2005, 352, 2477–2486. http://dx.doi.org/10.1056/NEJMoa042973[Crossref]
  • [8] Walsh S.W., Eicosanoids in preeclampsia, Prostag. Leukotr. Ess., 2004, 70, 223–232 http://dx.doi.org/10.1016/j.plefa.2003.04.010[Crossref]
  • [9] Parnas M., Sheiner E., Shoham-Vardi I., Burstein E., Yermiahu T., Levi I., et al., Moderate to severe thrombocytopenia during pregnancy, Eur. J. Obstet. Gynecol. Rep. Biol., 2006, 128, 163–168 http://dx.doi.org/10.1016/j.ejogrb.2005.12.031[Crossref]
  • [10] McCrae K.R., Thrombocytopenia in pregnancy: differential diagnosis, pathogenesis, and management, Blood Rev., 2003, 17, 7–14 http://dx.doi.org/10.1016/S0268-960X(02)00056-5[Crossref]
  • [11] Fallahian M., Nabaie F., Subclinical thrombocytopenia and preeclampsia, Int. J. Gynecol. Obstet., 2005, 89, 47–48 http://dx.doi.org/10.1016/j.ijgo.2004.12.033[Crossref]
  • [12] Ceyhan T., Beyan C., Başer I., Kaptan K., Güngör S., Ifran A., The effect of pre-eclampsia on complete blood count, platelet count and mean platelet volume, Ann. Hematol., 2006, 85, 320–322 http://dx.doi.org/10.1007/s00277-006-0091-7[Crossref]
  • [13] Hutt R., Ogunntui S.O., Sullivan M.H., Elder M.G., Increased platelet volume and aggregation precedes the onset of preeclampsia, Obstet. Gynecol., 1994, 83, 146–149
  • [14] Norris L.A., Gleeson N., Sheppard B.I., Bonnar J., Whole blood platelet aggregation in moderate and severe pre-eclampsia, Br. J. Obstet. Gynecol., 1993, 100, 684–688 http://dx.doi.org/10.1111/j.1471-0528.1993.tb14239.x[Crossref]
  • [15] Bowry S.K., Prentice C.R., Courtney J.M., A modification of the Wu and Hoak method for the determination of platelet aggregates and platelet adhesion, Thromb. Haemost., 1985, 53, 381–385
  • [16] Rogner G., Turbidimetric measurement of thrombocytes aggregation using small blood quantities, Z. Med. Labortech., 1974, 15, 225–231, (in German)
  • [17] Faul F., Erdfelder E., Buchner A., Lang A.G., Statistical power analyses using G*Power 3.1: Tests for correlation and regression analyses, Behav. Res. Methods, 2009, 41, 1149–1160 http://dx.doi.org/10.3758/BRM.41.4.1149[WoS][Crossref]
  • [18] Faul F., Erdfelder E., Lang A.G., Buchner A., G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences, Behav. Res. Methods, 2007, 39, 175–191 http://dx.doi.org/10.3758/BF03193146[Crossref]
  • [19] Machin D., Campbell M.J., Walters S.J., Medical statistics: a textbook for the health sciences, 4th ed., John Wiley & Sons, Chichester, U.K., 2007
  • [20] Burrows R.F., Kelton J.G., Incidentally detected thrombocytopenia in healthy mothers and their infants, N. Engl. J. Med., 1988, 319, 142–148 http://dx.doi.org/10.1056/NEJM198807213190304[Crossref]
  • [21] Matthews J.H., Benjamin S., Gill D.S., Smith N.A., Pregnancy - associated thrombocytopenia: Definition, incidence and natural history, Acta Haematol., 1990, 84, 24–29 http://dx.doi.org/10.1159/000205022[Crossref]
  • [22] Young B.C., Levine R.J., Karumanchi S.A., Pathogenesis of preeclampsia, Annu. Rev. Pathol., 2010, 5, 173–192 http://dx.doi.org/10.1146/annurev-pathol-121808-102149[Crossref]
  • [23] McFarland J., Pathophysiology of platelet destruction in immune (idiopathic) thrombocytopenic purpura, Blood Rev., 2002, 16, 1–2 http://dx.doi.org/10.1054/blre.2001.0169[Crossref]
  • [24] Socol M.L., Weiner C.P., Louis G., Rehnberg K., Rossi E.C., Platelet activation in preeclampsia, Am. J. Obstet. Gynecol., 1985, 151, 494–497
  • [25] Kroll M.H., Schafer A.I., Biochemical mechanisms of platelet activation, Blood, 1989, 74, 1181–1195
  • [26] Siess W., Molecular mechanisms of platelet activation, Physiol. Rev., 1989. 69, 58–178
  • [27] Andrews R.K., López J.A., Berndt M.C., Molecular Mechanisms of Platelet Adhesion and Activation, Int. J. Biochem. Cell. Biol., 1997, 29, 91–105 http://dx.doi.org/10.1016/S1357-2725(96)00122-7[Crossref]
  • [28] Verhoeven A.J.M., Mommersteeg M.E., Akkerman J.W.N., Qunatification of energy consumption in platelets during thrombin-induced aggregation and secretion. Tight coupling between platelet responses and the increment in energy consumption, Biochem. J., 1984, 221, 777–787
  • [29] Yoneyama Y., Suzuki S., Sawa R., Takeuchi T., Kobayashi H., Takei R., et al., Changes in Plasma Adenosine Concentrations during Normal Pregnancy, Gynecol. Obstet. Invest., 2000, 50, 145–148 http://dx.doi.org/10.1159/000010313[Crossref]
  • [30] Takeuchi T., Yoneyama Y., Suzuki S., Sawa R., Otsubo Y., Araki T., Regulation of Platelet Aggregation in vitro by Plasma Adenosine in Preeclampsia, Gynecol. Obstet. Invest., 2001, 51, 36–39 http://dx.doi.org/10.1159/000052888[Crossref]
  • [31] Schwarz U.R., Waltera U., Eigenthalera M., Taming platelets with cyclic nucleotides, Biochem. Pharmacol., 2001, 62, 1153–1161 http://dx.doi.org/10.1016/S0006-2952(01)00760-2[Crossref]
  • [32] Liua F.C., Liao C.H., Chang Y.W., Liou J.T., Daya Y.J., A new insight of anti-platelet effects of sirtinol in platelets aggregation via cyclic AMP phosphodiesterase. Biochem. Pharmacol., 2009, 77, 1364–1373 http://dx.doi.org/10.1016/j.bcp.2009.01.011[WoS][Crossref]
  • [33] Babic G.M., Dragicevic-Dokovic L.M., Jakovljevic V.L., Maletic S.D., Kostic M.M., Endothelial Cell (Dis)Function in Preeclampsia, Medicus 2000;1: 44–45
  • [34] de Belder A.J., MacAllister R., Radomski M.W., Moncada S., Vallance P.J.T., Effects of S-nitrosoglutathione in the human forearm circulation: evidence for selective inhibition of platelet activation, Cardiovasc. Res., 1994, 28, 691–694 http://dx.doi.org/10.1093/cvr/28.5.691[Crossref]
  • [35] Cunningham F., Leveno K., Bloom S., Hauth J., Rouse D., Spong C., Williams Obstetrics, 23rd ed., McGraw-Hill Professional Publishing, USA, 2010

Document Type

Publication order reference


YADDA identifier

JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.