OCT 4 immunohistochemistry in postpubertal cryptorchidism

• Authors: Ivan Krhen , Tomislav Kulis , Marijana Coric , Nikola Knezevic , Zvonimir Marekovic , Zeljko Kastelan
• Languages of publication: EN

Abstracts

EN

Patients with cryptorchidism are at an increased risk for germ cell testicular cancer. OCT 4 has been shown to be a sensitive and specific marker for some types of germ cell testicular cancer. We undertook this study to establish whether OCT 4 immunohistochemistry is a useful tool in the pathohistologic evaluation of postpubertal patients with cryptorchidism. Seventeen postpubertal patients underwent orchidectomy for cryptorchidism at our center since 1997. Immunohistochemical staining with OCT 4 was performed on these samples. Characteristic OCT 4 nuclear staining was positive in two patients. One patient was correctly diagnosed on previous pathohistological evaluation, while OCT4 immunohistochemical staining revealed previously unidentified intratubular germ cell neoplasia in the other patient. OCT 4 immunohistochemistry can be useful in diagnosing a testicular germ cell tumor in patients with cryptorchidism. If we consider a low number of postpubertal patients with cryptorchidism a benefit of immunohistochemical staining with OCT4, this could favor the use of OCT 4 staining in work-up of cryptorchidism.

Keywords

EN

OCT4; OCT 3/4; POU5F1; Cryptorchidism; Immunohistochemistry; Testis neoplasia

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2011
• Volume: 6
• Issue: 2
• Pages: 243-246

Dates

published

1 - 4 - 2011

online

17 - 2 - 2011

Contributors

author

Ivan Krhen

• Department of Urology, University Hospital Center Zagreb and Zagreb University School of Medicine, 10000, Zagreb, Croatia

author

Tomislav Kulis

• Department of Urology, University Hospital Center Zagreb and Zagreb University School of Medicine, 10000, Zagreb, Croatia

author

Marijana Coric

• Department of pathology and cytology, University Hospital Center Zagreb and Zagreb University School of Medicine, Zagreb, Croatia

author

Nikola Knezevic

• Department of Urology, University Hospital Center Zagreb and Zagreb University School of Medicine, 10000, Zagreb, Croatia

author

Zvonimir Marekovic

• Department of Urology, University Hospital Center Zagreb and Zagreb University School of Medicine, 10000, Zagreb, Croatia

author

Zeljko Kastelan

• Department of Urology, University Hospital Center Zagreb and Zagreb University School of Medicine, 10000, Zagreb, Croatia

References

• [1] Curado. M. P. E., B., Shin. H.R., Storm. H., Ferlay. J., Heanue. M. and Boyle. P., ed. Cancer Incidence in Five Continents, Vol. IX. Lyon, IARC 2007
• [2] Cancer Incidence in Croatia 2003–2007. Bulletins No 27-31. Zagreb: Croatian National Institute of Public Health 2005–2009
• [3] Sant M., Aareleid T., Berrino F., Bielska Lasota M., Carli P.M., Faivre J., et al., EUROCARE-3: survival of cancer patients diagnosed 1990–94-results and commentary, Ann Oncol, 2003, 14Suppl 5, v61–118 http://dx.doi.org/10.1093/annonc/mdg754[Crossref]
• [4] Einhorn L.H., Donohue J., Cisdiamminedichloroplatinum, vinblastine, and bleomycin combination chemotherapy in disseminated testicular cancer, Ann Intern Med, 1977, 87, 293–298
• [5] Dieckmann K.P., Pichlmeier U., Clinical epidemiology of testicular germ cell tumors, World J Urol, 2004, 22, 2–14 http://dx.doi.org/10.1007/s00345-004-0398-8[Crossref]
• [6] Pettersson A., Richiardi L., Nordenskjold A., Kaijser M., Akre O., Age at surgery for undescended testis and risk of testicular cancer, N Engl J Med, 2007, 356, 1835–1841 http://dx.doi.org/10.1056/NEJMoa067588[Crossref][WoS]
• [7] Hutson J.M., Hasthorpe S., Testicular descent and cryptorchidism: the state of the art in 2004, J Pediatr Surg, 2005, 40, 297–302 http://dx.doi.org/10.1016/j.jpedsurg.2004.10.033[Crossref]
• [8] Rogers E., Teahan S., Gallagher H., Butler M.R., Grainger R., McDermott T.E., et al., The role of orchiectomy in the management of postpubertal cryptorchidism, J Urol, 1998, 159, 851–854 http://dx.doi.org/10.1016/S0022-5347(01)63752-2[Crossref]
• [9] Faulkner S.W., Leigh D.A., Oosterhuis J.W., Roelofs H., Looijenga L.H., Friedlander M.L., Allelic losses in carcinoma in situ and testicular germ cell tumours of adolescents and adults: evidence suggestive of the linear progression model, Br J Cancer, 2000, 83, 729–736 http://dx.doi.org/10.1054/bjoc.2000.1334[Crossref]
• [10] Giwercman A., Bruun E., Frimodt-Moller C., Skakkebaek N.E., Prevalence of carcinoma in situ and other histopathological abnormalities in testes of men with a history of cryptorchidism, J Urol, 1989, 142, 998–1001: discussion 1001–1002
• [11] Krabbe S., Skakkebaek N.E., Berthelsen J.G., Eyben F.V., Volsted P., Mauritzen K., et al., High incidence of undetected neoplasia in maldescended testes, Lancet, 1979, 1, 999–1000 http://dx.doi.org/10.1016/S0140-6736(79)92754-5[Crossref]
• [12] Schottenfeld D., Cancer epidemiology and prevention, New York, Oxford University Press, 1996
• [13] Rosner M.H., Vigano M.A., Ozato K., Timmons P.M., Poirier F., Rigby P.W., et al., A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo, Nature, 1990, 345, 686–692 http://dx.doi.org/10.1038/345686a0[Crossref]
• [14] Scholer H.R., Dressler G.R., Balling R., Rohdewohld H., Gruss P., Oct-4: a germline-specific transcription factor mapping to the mouse t-complex, EMBO J, 1990, 9, 2185–2195
• [15] Looijenga L.H., Stoop H., de Leeuw H.P., de Gouveia Brazao C.A., Gillis A.J., van Roozendaal K.E., et al., POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors, Cancer Res, 2003, 63, 2244–2250
• [16] van Casteren N.J., Boellaard W.P., Dohle G.R., Weber R.F., Kuizinga M.C., Stoop H., et al., Heterogeneous distribution of ITGCNU in an adult testis: consequences for biopsy-based diagnosis, Int J Surg Pathol, 2008, 16, 21–24 http://dx.doi.org/10.1177/1066896907306125[Crossref][WoS]
• [17] Oh J., Landman J., Evers A., Yan Y., Kibel A.S., Management of the postpubertal patient with cryptorchidism: an updated analysis, J Urol, 2002, 167, 1329–1333 http://dx.doi.org/10.1016/S0022-5347(05)65293-7[Crossref]
• [18] Jones T.D., Ulbright T.M., Eble J.N., Cheng L., OCT4: A sensitive and specific biomarker for intratubular germ cell neoplasia of the testis, Clin Cancer Res, 2004, 10, 8544–8547 http://dx.doi.org/10.1158/1078-0432.CCR-04-0688[Crossref]
• [19] Cools M., Stoop H., Kersemaekers A.M., Drop S.L., Wolffenbuttel K.P., Bourguignon J.P., et al., Gonadoblastoma arising in undifferentiated gonadal tissue within dysgenetic gonads, J Clin Endocrinol Metab, 2006, 91, 2404–2413 http://dx.doi.org/10.1210/jc.2005-2554[Crossref]
• [20] Cheng L., Establishing a germ cell origin for metastatic tumors using OCT4 immunohistochemistry, Cancer, 2004, 101, 2006–2010 http://dx.doi.org/10.1002/cncr.20566[Crossref]
• [21] Ulbright T.M., Germ cell tumors of the gonads: a selective review emphasizing problems in differential diagnosis, newly appreciated, and controversial issues, Mod Pathol, 2005, 18Suppl 2, S61–79 http://dx.doi.org/10.1038/modpathol.3800310[Crossref]
• [22] Rajpert-De Meyts E., Hanstein R., Jorgensen N., Graem N., Vogt P.H., Skakkebaek N.E., Developmental expression of POU5F1 (OCT-3/4) in normal and dysgenetic human gonads, Hum Reprod, 2004, 19, 1338–1344 http://dx.doi.org/10.1093/humrep/deh265[Crossref]

Identifiers

DOI

10.2478/s11536-010-0074-x