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2011 | 6 | 1 | 58-63
Article title

The effect of vitamin C on amiodarone-induced toxicity in rat thymocytes

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Abstracts
EN
Although, the antiarrhythmic effect of amiodarone (AMD) is well characterized, the mechanism of its toxicity on extracardiac tissues is still poorly understood. Several antioxidants have been shown to prevent AMD-induced toxicity by antioxidant and/or non-antioxidant mechanisms. In the current study, we evaluated the possible protective effect, in vitro, of vitamin C on AMD-induced toxicity in rat thymocytes. Rat thymocytes were cultured with increasing AMD concentrations (1–20 μM) with or without vitamin C (1000 μg/ml), for 24 hours. Cells treatment with AMD resulted in a concentration-dependent increase of hypodiploid cells and a significant decrease in cellular glutathione content. Vitamin C combined with AMD significantly decreased the proportion of hypodiploid cells and markedly increased the cellular glutathione content, compared with AMD treatment alone. These results suggest that treatment with vitamin C may prevent AMD-induced toxicity in rat thymocytes by restoring cellular glutathione content.
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Publisher

Journal
Year
Volume
6
Issue
1
Pages
58-63
Physical description
Dates
published
1 - 2 - 2011
online
16 - 12 - 2010
Contributors
author
  • Institute of Physiology, Medical Faculty University in Nis, 18000, Nis, Serbia
author
  • Institute of Biochemistry, Medical Faculty University in Nis, 18000, Nis, Serbia
author
  • Medical Faculty University in Nis, 18000, Nis, Serbia
author
  • Clinic of Pediatrics, Medical Faculty University in Nis, 18000, Nis, Serbia
author
  • Institute for Prevention, Treatment and Rehabilitation of Rheumatic and Cardiovascular Diseases, Medical Faculty University in Nis, 18000, Nis, Serbia
author
  • Institute of Physiology, Medical Faculty University in Nis, 18000, Nis, Serbia, vojapav@yahoo.com
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11536-010-0050-5
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