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2010 | 5 | 1 | 30-35

Article title

Mutations leading to drug-resistant Mycobacterium tuberculosis infection in Ukraine


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The goal of this research was detection of the drug-resistance level of Mycobacterium tuberculosis infection in the Odesa region of Southwest Ukraine, investigation of the level of mutation in katG and rpoB genes for M. tuberculosis with polymerase chain reaction (PCR), and spread of these mutations in different groups of patients with tuberculosis. An extremely high level of primary and acquired resistance of M. tuberculosis to first-line antituberculosis drugs has been found in the Southwest region of Ukraine. The PCR method has proved to have high sensitivity in the detection of mutations in katG and rpoB genes. The data showed significant spreading of M. tuberculosis strains with mutations in katG and rpoB genes in penitentiaries and an increased level of these mutations during tuberculosis treatment. The presence of mutations in rpoB and katG genes was associated with a more severe course of tuberculosis, increased risk of treatment default, persistence of positive smears on microscopy at discharge, and poor closing of tuberculous cavities. Extremely high level of mutations in the rpoB and katG genes of M. tuberculosis was observed in Beijing family strains. Our findings support the capability of the PCR method to detect M. tuberculosis that is resistant to drugs such as isoniazid and rifampicin.











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1 - 2 - 2010
29 - 1 - 2010


  • Department of General and Clinical Pharmacology, Odesa State Medical University, 65082, Odesa, Ukraine
  • Department of General and Clinical Pharmacology, Odesa State Medical University, 65082, Odesa, Ukraine
  • Department of General and Clinical Pharmacology, Odesa State Medical University, 65082, Odesa, Ukraine


  • [1] Mariandyshev A., Nizovtseva N., Panasik V.; Challenges of MDR-TB control in Archangelsk Russian Federation penitentiary system, The International journal of tuberculosis and lung diseases, 2007, 11(suppl. 1), 5
  • [2] Anti-tuberculosis drug resistance in the world, 4th Global Report The WHO/IUATLD Global Project on Anti-tuberculosis Drug Resistance Surveillance 2002–2007., Geneva, 2007
  • [3] Mokrousov I., Otten T., Filipenko M.; Detection of Isoniazid-Resistant Mycobacterium tuberculosis Strains by a Multiplex Allele-Specific PCR Assay Targeting katG Codon 315 Variation, Journal of Clinical Microbiology, 2002, 40, 2509–2512 http://dx.doi.org/10.1128/JCM.40.7.2509-2512.2002[Crossref]
  • [4] Mokrousov I., Otten T., Vyshnevskiy B.; Allele-Specific rpoB PCR Assays for Detection of Rifampin-Resistant Mycobacterium tuberculosis in Sputum Smears, Antimicrobial agents and chemotherapy, 2003, 47, 2231–2235 http://dx.doi.org/10.1128/AAC.47.7.2231-2235.2003[Crossref]
  • [5] Robert H. Fletcher, Suzanne W. Fletcher, Clinical epidemiology: the essentials, 4th. Ed., Lippincott Williams & Wilkins, 2005
  • [6] Патент №29211 Украïни, МПК (2006) А61К 31/00; С12Q 1/68; С12R 1/32 Склад реактивноï сумiщi для длагностики резистентностi до iзон∈азиду збудника туберкульозу: Антоненко К.О., Кресюн, В.Й, Антоненко П.Б. (Украïна) - Заявл. 30.07.2007; Опубл. 10.01.2008, Бюл. №1. - с.10. (in Ukrainian)
  • [7] Banu S., Stephen V. Gordon, Si Palmer; Genotypic Analysis of Mycobacterium tuberculosis in Bangladesh and Prevalence of the Beijing Strain, J. Clin. Microbiol., 2004, 42, 674–682 http://dx.doi.org/10.1128/JCM.42.2.674-682.2004[Crossref]
  • [8] Нiкола∈вський В.В., Кресюн В.Й., Пруднiкова К.О.; Показники стiйкостi мiкобактерiй туберкульозу до протитуберкульозних препаратiв перщого ряду в м. Одесi та Одеськiй областi, Одеський медичний журнал, 2004, 3, 59–63 (in Ukrainian)
  • [9] Global tuberculosis control: WHO Report 2002., Geneva, 2002
  • [10] Lyepshina S.M., Yann Y.V., Dubrovina I.V.; Association between multidrug-resistant tuberculosis and HIV infection in Donetsk Oblast, Ukraine, The International journal of tuberculosis and lung diseases, 2007, № 11 (suppl. 1), 108
  • [11] Nikolayevskyy V.V., Brown T.J., Bazhora Y.I.; Molecular epidemiology and prevalence of mutations conferring rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains from the southern Ukraine, Clin. Microbiol. Infect., 2007, 13, 129–138 http://dx.doi.org/10.1111/j.1469-0691.2006.01583.x[Crossref]
  • [12] Николаевский В.В. Дробневски Ф.А., Бажора Ю.И.; Молекулярно-генетическая характеристика щтаммов микобактерий, выделенных в Южном регионе Украины, Цитология и генетика, 2004, 4, 29–33 (in Russian)
  • [13] Kazenny B.Y., Khorosheva T.M., Kiryanova E.V.; Results of a DOTS-Plus project in Orel Oblast, Russian Federation, November 2002–December 2006, The International journal of tuberculosis and lung diseases, 2007, № 11 (suppl. 1), 174
  • [14] No authors listed; Tuberculosis and HIV infection: detection, follow-up, treatment: Moscow data, Probl. Tuberk. Bolezn. Legk., 2007, 10, 31–36
  • [15] Dabrowska M.M., Pogorzelska J., Parfieniuk A.; Tuberculosis and HIV infection-main diagnostic and therapeutic problems, Pol. Merkur. Lekarski., 2007, 135, 212–214

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