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2010 | 5 | 1 | 108-114

Article title

Does diabetes affect the intensity of staining of interstitial cells and neuronal tissue in the bladder, prostate and urethra of rabbits?


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We compared the intensity of staining of interstitial cells (ICs) and neural tissue in the lower urinary tract of rabbits with diabetes with the intensity in normal subjects. Diabetes was induced by injecting alloxane (65mg/kg) in adult male rabbits. After 3 days, rabbits with a blood glucose level >300 mg/dL were considered to have diabetes. After 8 weeks, the rabbits were killed, and tissue specimens from the bladder, prostate and urethra were obtained. ICs were stained with anti-human CD117 (c-kit) rabbit polyclonal antibody, and neural tissue was stained with synaptophysin. The streptavidin-biotin method was used for immunohistochemical staining. The intensity of c-kit and synaptophysin staining were scored as negative (0), weak (+), moderate (++), and strong (+++). Staining intensity of ICs and neural tissue was assessed and compared in tissues obtained from rabbits with diabetes (n=8) and from control subjects (n=7). Although staining intensity of both ICs and neural tissue was found to be significantly decreased in the bladder tissue of rabbits with diabetes compared to that in the control group (p=0.0001 [ICs] and p=0.021 [neural tissue]), no significant differences in staining intensity of ICs and neural tissue in the urethra and in the prostate was found when rabbits with diabetes were compared to the control group. Diabetes may cause dysfunction of the lower urinary tract, particularly in the urinary bladder, as shown by the staining intensity of ICs and neural tissue.










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1 - 2 - 2010
29 - 1 - 2010


  • 1st Urology Department, Ankara Atatürk Training and Research Hospital, 06800, Ankara, Turkey
  • Institute of Oncology, Department of Basic Oncology, Dokuz Eylül University, 35310, Izmir, Turkey
  • Department of Urology, Ege University School of Medicine, 35100, Izmir, Turkey
  • Department of Pharmacology and Clinical Pharmacology, Ege University, School of Medicine, 35100, Izmir, Turkey


  • [1] Aubert R.E., Geiss L.S., Ballard D.J., Cocanougher B., Herman W.H., Diabetes related hospitalization and hospital utilization., In: Diabetes in America, 2nd Edition: National Institutes of Health; 553–569,1995
  • [2] Poladia D.P., Schanbacher B., Wallace L.J., Bauer J.A., Innervation and connexin isoform expression during diabetes-related bladder dysfunction: early structural vs. neuronal remodeling, Acta Diabetol., 2005,42(3),147–152 http://dx.doi.org/10.1007/s00592-005-0194-y[Crossref]
  • [3] Kubota Y., Nakahara T., Mitani A., Maruko T., Sakamoto K., Ishii K., Augmentation of rat urinary bladder relaxation mediated by beta1-adrenoceptors in experimental diabetes, Eur J Pharmacol., 2003,467(1–3),191–195 http://dx.doi.org/10.1016/S0014-2999(03)01630-3
  • [4] Su X., Changolkar A., Chacko S., Moreland R.S., Diabetes decreases rabbit bladder smooth muscle contraction while increasing levels of myosin light chain phosphorylation, Am J Physiol Renal Physiol., 2004,287(4),690–699 http://dx.doi.org/10.1152/ajprenal.00027.2004[Crossref]
  • [5] Liu G., Lin Y.H., Yamada Y., Daneshgari F., External urethral sphincter activity in diabetic rats, Neurourol Urodyn., 2008,27(5),429–434 http://dx.doi.org/10.1002/nau.20543[Crossref]
  • [6] Yang Z., Dolber P.C., Fraser M.O., Diabetic urethropathy compounds the effects of diabetic cystopathy, J Urol., 2007,178(5),2213–2219 http://dx.doi.org/10.1016/j.juro.2007.06.042[Crossref]
  • [7] Torimoto K., Fraser M.O., Hirao Y., De Groat W.C., Chancellor M.B., Yoshimura N., Urethral dysfunction in diabetic rats, J Urol., 2004,171(5),1959–19564 http://dx.doi.org/10.1097/01.ju.0000121283.92963.05[Crossref]
  • [8] van der AA F., Roskams T., Blyweert W., Ost D., Bogaert G., De Ridder D., Identification of kit positive cells in the human urinary tract, J Urol., 2004,171(6 Pt 1),2492–2496
  • [9] Wiseman O.J., Fowler C.J., Landon D.N., The role of the human bladder lamina propria myofibroblast, BJU Int., 2003,91(1),89–93 http://dx.doi.org/10.1046/j.1464-410X.2003.03802.x[Crossref]
  • [10] Shafik A., El-Sibai O., Shafik A.A., Shafik I., Identification of interstitial cells of Cajal in human urinary bladder: concept of vesical pacemaker, Urology., 2004,64(4),809–813 http://dx.doi.org/10.1016/j.urology.2004.05.031[Crossref]
  • [11] Sergeant G.P., Hollywood M.A., McCloskey K.D., Thornbury K.D., McHale N.G.,Specialised pacemaking cells in the rabbit urethra, J Physiol., 2000,526,359–366 http://dx.doi.org/10.1111/j.1469-7793.2000.t01-2-00359.x[Crossref]
  • [12] Seifert P., Benedic M., Effert P., Nerve fibers in tumors of the human urinary bladder, Virchows Arch., 2002,440(3),291–297 http://dx.doi.org/10.1007/s004280100496[Crossref]
  • [13] Felkl M., Leube R.E., Interaction assays in yeast and cultured cells confirm known and identify novel partners of the synaptic vesicle protein synaptophysin, Neuroscience., 2008,156(2),344–352 http://dx.doi.org/10.1016/j.neuroscience.2008.07.033[WoS][Crossref]
  • [14] Wu J., Ohlsson M., Warner E.A., Loo K.K., Hoang T.X., Voskuhl R.R., et al., Glial reactions and degeneration of myelinated processes in spinal cord gray matter in chronic experimental autoimmune encephalomyelitis, Neuroscience., 2008,156(3),586–596 http://dx.doi.org/10.1016/j.neuroscience.2008.07.037[WoS][Crossref]
  • [15] Hermann G.G., Andersen C.B., Transitional cell carcinoma Express vitamin D receptors, Scand J Urol Nephrol., 1996,31,161–166 http://dx.doi.org/10.3109/00365599709070323[Crossref]
  • [16] Ristimaki A., Honkanen N., Jankala H., Sipponen P., Harkönen M., Expression of cyclooxygenase-2 in human gastric carcinoma, Cancer Res., 1997,57,1276–1280
  • [17] Metzger R., Neugebauer A., Rolle U., Böhlig L., Till H., C-Kit receptor (CD117) in the porcine urinary tract, Pediatr Surg Int., 2008,24(1),67–76. http://dx.doi.org/10.1007/s00383-007-2043-2[Crossref]
  • [18] Kubota Y., Hashitani H., Shirasawa N., Kojima Y., Sasaki S., Mabuchi Y., Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction, Neurourol Urodyn., 2008,27(4),:330–340 http://dx.doi.org/10.1002/nau.20502[Crossref]
  • [19] Højlund K., Mogensen M., Sahlin K., Beck-Nielsen H., Mitochondrial dysfunction in type 2 diabetes and obesity, Endocrinol Metab Clin North Am., 2008,37(3),713–731 http://dx.doi.org/10.1016/j.ecl.2008.06.006
  • [20] Abdul-Ghani M.A., DeFronzo R.A., Mitochondrial dysfunction, insulin resistance, and type 2 diabetes mellitus, Curr Diab Rep., 2008,8(3),173–178 http://dx.doi.org/10.1007/s11892-008-0030-1
  • [21] McCloskey KD., Characterization of outward currents in interstitial cells from the guinea pig bladder, J Urol., 2005,173,296–301 http://dx.doi.org/10.1097/01.ju.0000141581.00922.f4[Crossref]
  • [22] Canda AE., Editorial Comment on: Molecular Mechanisms Related to Parturition-Induced Stress Urinary Incontinence, Eur Urol., 2008,Mar 18, [Epub ahead of print]
  • [23] Canda A.E., Cross R.L., Chapple C.R., Pharmacology of the lower urinary tract and management of overactive bladder, J Turkish-German Gynecol Assoc., 2006,7,146–158
  • [24] Canda A.E., Cinar M.G., Turna B., Sahin M.O., Pharmacologic targets on the female urethra, Urol Int., 2008,80(4),341–354 http://dx.doi.org/10.1159/000132690[Crossref]
  • [25] Edwards F.R., Hirst G.D., Suzuki H., Unitary nature of regenerative potentials recorded from circular smooth muscle of guinea-pig antrum, J Physiol., 1999,519,235–250 http://dx.doi.org/10.1111/j.1469-7793.1999.0235o.x[Crossref]
  • [26] Sanders K.M., A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract, Gastroenterology., 1996,111,492–515 http://dx.doi.org/10.1053/gast.1996.v111.pm8690216[Crossref]
  • [27] Collins C., Klausner A.P., Herrick B., Ko H.P., Miner A.S., Henderson S.C., Ratz P.H., Potential for control of detrusor smooth muscle spontaneous rhythmic contraction by cyclooxygenase products released by interstitial cells of Cajal, J Cell Mol Med., 2009, Feb 20, [Epub ahead of print] [Crossref][WoS]
  • [28] Johnston L., Carson C., Lyons A.D., Davidson R.A., McCloskey K.D., Cholinergic-induced Ca2+ signaling in interstitial cells of Cajal from the guinea pig bladder, Am J Physiol Renal Physiol., 2008,294(3),645–655 http://dx.doi.org/10.1152/ajprenal.00526.2007[Crossref][WoS]
  • [29] Lyons A.D., Gardiner T.A., McCloskey K.D., Kit-positive interstitial cells in the rabbit urethra: structural relationships with nerves and smooth muscle, BJU Int. 2007,99(3),687–694 http://dx.doi.org/10.1111/j.1464-410X.2006.06617.x[Crossref][WoS]
  • [30] McCloskey K.D., Calcium currents in interstitial cells from the guinea-pig bladder, BJU Int., 2006,97(6),1338–1343 http://dx.doi.org/10.1111/j.1464-410X.2006.06156.x[Crossref]
  • [31] Nobe K., Yamazaki T., Tsumita N., Hashimoto T., Honda K., Glucose-dependent enhancement of diabetic bladder contraction is associated with a rho kinase-regulated protein kinase C pathway, J Pharmacol Exp Ther., 2009,328(3),940–950 http://dx.doi.org/10.1124/jpet.108.144907[WoS][Crossref]
  • [32] Bradley W.E., Diagnosis of urinary bladder dysfunction in diabetes mellitus, Ann Intern Med 1980,92,323–326 [Crossref]
  • [33] Mitsui T., Kakizaki H., Kobayashi S., Morita H., Matsumura K., Koyanagi T., Vesicourethral function in diabetic patients: association of abnormal nerve conduction velocity with vesicourethral dysfunction, Neurourol Urodyn., 1999,18:639–645 http://dx.doi.org/10.1002/(SICI)1520-6777(1999)18:6<639::AID-NAU14>3.0.CO;2-R[Crossref]
  • [34] Andersson K.E., Treatment-resistant detrusor overactivity-underlying pharmacology and potential mechanisms, Int J Clin Pract Suppl., 2006,(151),8–16

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