Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl


Preferences help
enabled [disable] Abstract
Number of results


2009 | 4 | 2 | 198-202

Article title

The current analysis of the effect of hyperbaric oxygen therapy on the frostbitten tissue: Experimental study in rabbits


Title variants

Languages of publication



Many experimental studies have been performed and the mechanism of hyperbaric oxygen therapy on the frostbitten tissue has not been elucidated. In this study, we evaluated the effect of hyperbaric oxygen therapy on the frostbitten ears of rabbits in an experimental animal model by examining the concentrations of thromboxane A2 (as thromboxane B2-Tx B2) and of prostaglandin I2 (PG I2) (as 6-keto-prostaglandin F1α-PG F1α) in tissues, and by counting the numbers of inflammatory cells (neutrophils and mast cells-MC) Hyperbaric oxygen therapy (HBO) at 2.5 ATA for 90 minutes twice daily for fourteen days to rabbits, the ears of which were subjected to frostbite, decreased presence of inflammatory cells (mast cells −75%; neutrophils −40%) and increased prostaglandin I2 (PG I2) (as 6-Keto-PGF1α) in the involved skin. Thromboxane A2 (TxA2) (as Tx B2) was unaffected. Our results revealed that an inflammatory process was the underlying cause of frostbite injury and that hyperbaric oxygen therapy was active in pathological situations involving an inflammatory process in frostbite.










Physical description


1 - 6 - 2009
27 - 3 - 2009


  • Department of Plastic and Reconstructive Surgery and Burn Unit, Gülhane Military Medical Academy and Medical Faculty, Haydarpasha Training Hospital, 34668, Istanbul, Turkey
  • Department of Plastic and Reconstructive Surgery and Burn Unit, Gülhane Military Medical Academy and Medical Faculty, Haydarpasha Training Hospital, 34668, Istanbul, Turkey
  • Department of Plastic and Reconstructive Surgery and Burn Unit, Gülhane Military Medical Academy and Medical Faculty, Haydarpasha Training Hospital, 34668, Istanbul, Turkey
  • Department Of Undersea and Hyperbaric Medicine, Kasimpasha Military Hospital, 34440, Istanbul, Turkey


  • [1] Herndon D. Cold Induced Injury: frostbite. In: Herndon D. Total Burn Care, 2 edn. London: Saunders 2002: 470
  • [2] Ozyazgan I, Tercan M, Bekerecioğlu M, Melli M, Ustün H, Günay GK. Defibrotide activity in experimental frostbite injury. Br J Plast Surg. 1998 Sep;51(6):450–4
  • [3] Weathearly-White R., Sjostrom B., Patom B. Experimental studies in cold injury II: The pathogenesis of Frostbite. J Surg Res. 1964; 4: 17–22 http://dx.doi.org/10.1016/S0022-4804(64)80004-4[Crossref]
  • [4] Weathearly-White R., Sjostrom B., Patom B. Experimental studies in cold injury III: Observations on the Treatment of Frostbite. Plast. Reconstr. Surg. 1965; 36: 10–8 http://dx.doi.org/10.1097/00006534-196507000-00004[Crossref]
  • [5] Quintanilla R., Krusen F., Essex H. Studies on Frostbite with Special Referance to Treatment and Effect on Minute Blood Vessels. Am. J. Physiol. 1947; 149: 149–61
  • [6] Eubanks R. Heat and Cold Injuries. J. Arkansas Med. Soc. 1974; 71: 53–8
  • [7] Furhman F., Crismon J. Studies on Gangrene Following Cold Injury, II. General Course of Events in Rabbit Feet and Ears Following Untreated Cold Injury. J. Clin. Invest. 1947; 47: 236–44 http://dx.doi.org/10.1172/JCI101801[Crossref]
  • [8] Rasmussen D., Zook E. Frosbite: A Review of Pathophysiology and Newest Treatments. J. Indiana State Med. Assoc. 1972; 65: 1237–41
  • [9] Welch G. Frostbite. Practitioner. 1974; 213: 801–4
  • [10] Merryman H. Mechanics of Freezing In Living Cells and Tissue. Science. 1956; 124: 515–21 http://dx.doi.org/10.1126/science.124.3221.515[Crossref]
  • [11] Moran T. Critical temperature of freezing living muscle. Proc. R. Soc. B 1929; 105:107 http://dx.doi.org/10.1098/rspb.1929.0036[Crossref]
  • [12] Merryman HT. The exceeding of a minimum tolerable cell volume in hypertonic suspension as a cause of freezing injury. In: Walstenholme G, O'Conner M, eds. The Frozen Cell. London: Churchill, 1970: 51 http://dx.doi.org/10.1002/9780470719732.ch4[Crossref]
  • [13] Shikama K., Yamazaki I. Deanturation of Catalase by Freezing and Thawing. Nature. 1961; 190
  • [14] Marhert C. Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits. Science. 1963; 140: 1629
  • [15] Lovelock J.E. Physical Instability In Thermal Shock in red Blood Cells. Nature. 1954; 173: 659 http://dx.doi.org/10.1038/173659a0[Crossref]
  • [16] Lovelock J.E. The Denaturation of Lipid-Protein Complexes as a Cause of Damage by Freezing. Proc. R. Soc. Biol. 1957; 147: 427 http://dx.doi.org/10.1098/rspb.1957.0062[Crossref]
  • [17] Mazur P. Studies in Rapidly Frozen Suspension of Yeast Cell by Differential Thermal Analysis and Conductometry. Biophys. J. 1963; 3: 323 http://dx.doi.org/10.1016/S0006-3495(63)86824-1[Crossref]
  • [18] Mazur P. Causes of injury in frozen and thawed cells. Fed Proc 1965; 24(suppl. 14–15): 5
  • [19] Korthuis RJ, Anderson DC, Granger DN. Role of neutrophil-endothelial cell adhesion in inflammatory disorders. J Crit Care. 1994 Mar;9(1):47–71 http://dx.doi.org/10.1016/0883-9441(94)90032-9[Crossref]
  • [20] Mileski W. J., Raymond J. F., Winn R. K., Harlan J. M., Rice C. L. Inhibition of Leukocyte Adherence and Aggregation for Treatment of Severe Cold Injury in Rabbits. J. Appl. Physiol. 1993; 74: 1432
  • [21] Ozyazgan I., Tercan M., Melli M., Bekerecioglu M., Ustun H., Gunay G. K. Eicosanoids and Inflammatory Cells in Frostbitten Tissue: Prostacyclin, Thromboxane, Polymorphonuclear Leukocytes, and Mast Cells. Plast. Reconstr. Surg. 1998; 101(7): 1881–6 http://dx.doi.org/10.1097/00006534-199806000-00016[Crossref]
  • [22] Waris T., and Kyösola K. Cold injury of the rat skin. Scand. J. Plasti Reconstr. Surg. 16:1, 1982 http://dx.doi.org/10.3109/02844318209006563[Crossref]
  • [23] Robson M. C., Heggers J. P. Effect of Hand Frostbite Blister Fluid as a Clue to Pathogenesis. J. Hand. Surg. 1981; 6: 43
  • [24] Schrör K., Köhler P., Müler M., Peskar B. A., Rösen P. Prostacyclin-Thromboxane Interactions in the Platelet-perfused in vitro Heart. Am. J. Physiol. 1981; 241: H18
  • [25] Robson M. C., Del Beccaro E. J., Heggers J. P. The Effects of Prostaglandins on the Dermal Microcirculation after Burning and the Inhibitions of the Effect by Specific Pharmacological Agents. Plast. Reconstr. Surg. 1979; 63: 781 http://dx.doi.org/10.1097/00006534-197963060-00003[Crossref]
  • [26] Del Baccaro E. J., Robson M. C., Heggers J. P., Swaminathan R. The Use of Thromboxane Inhibitors to Preserve the Dermal Microcirculation After Burning. Surgery. 1980; 87: 137
  • [27] Schrör K. Cytoprotection by Prostacyclins in Myocardial Ischemia. In U. Zor. Naor, and A. Danon (Eds), New Trend in Lipid Mediators Research, Vol. 3. Basel: Karger, 1989
  • [28] Heggers JP, Robson MC, Manavalen K, Weingarten MD, Carethers JM, Boertman JA, Smith DJJr, Sachs RJ. Eann Emerg Med. 1987 Sep;16(9):1056–62. Experimental and clinical observations on frostbite http://dx.doi.org/10.1016/S0196-0644(87)80758-8[Crossref]
  • [29] Jain KK. Indications, Contraindications, and Complications of HBO Therapy. In: Jain KK, ed. Textbook of Hyperbaric Medicine, 4th ed. Germany: Hogrefe & Huber Publishers Inc;2004:73–79

Document Type

Publication order reference


YADDA identifier

JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.