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Journal

2008 | 3 | 4 | 470-474

Article title

The comparison of cultures, widal agglutination test and polymerase chain reaction as a diagnostic tool in typhoid fever

Content

Title variants

Languages of publication

EN

Abstracts

EN
Typhoid fever caused by Salmonella typhi, paratyphi A and B, is an important cause of morbidity and mortality in many developing countries. A rapid and sensitive method for the detection of S. typhi is essential for early diagnosis of typhoid fever and effective therapy. In this study 45 febrile patients who were suspected to have enteric fever were enrolled, and the results of blood cultures, widal agglutination tests and Polymerase Chain Reaction in these cases were evaluated. Group I consisted of 11 patients with diseases other than salmonella infections, group II represented 6 patients with positive cultures, and group III represented 28 patients with negative blood cultures negative but who were clinically suspected cases that had a medical history of using variable antimicrobial agents. Two positive PCR results were present; one of them was in culture positive group (16,6%) and the other was in culture negative group (3,5%). In our study widal agglutination tests and cultures were found not to be helpful in differential dignosis. Although PCR based detection of S. typhi is reported to be a sensitive and specific test for the diagnosis of enteric fever, in our study the benefit of this method in the diagnosis of especially patients who were treated with antimicrobial therapy was not clearly determined. Other methods to increase sensitiviy and specificity to levels such as those of real time PCR should be developed and large-scaled studies should be done in endemic and non-epidemic regions.

Publisher

Journal

Year

Volume

3

Issue

4

Pages

470-474

Physical description

Dates

published
1 - 12 - 2008
online
22 - 10 - 2008

Contributors

author
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Hacettepe University, Turkey, Ankara
author
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
author
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
author
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
author
  • Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Hacettepe University, Turkey, Ankara
  • Department of Pediatrics, Hacettepe University, Faculty of Medicine, Turkey, Ankara
  • Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Hacettepe University, Turkey, Ankara

References

  • [1] Crump JA, Luby SP, Mintz ED. The global burden of typhoid fever. Bull World Health Organ 2004; 82: 346–53.
  • [2] Gasem MH,. Dolmans WM, Isbandrio BB et al. Culture of Salmonella typhi and Salmonella paratyphi from blood and bone marrow in suspected typhoid fever. Trop Geogr Med 1995; 47: 164–67.
  • [3] Wain J, Diep TS, Ho VA, et al. Quantitation of bacteria in blood of typhoid fever patients and relationship between counts and clinical features, transmissibility, and antibiotic resistance. J Clin Microbiol 1998; 36: 1683–87.
  • [4] Kumar A., Arora V., Bashampoo A et al. Detection of Salmonella typhi by polymerase chain reaction: Implications in diagnosis of typhoid fever. Infection, Genetics and Evolution 2 (2002) 107–110. http://dx.doi.org/10.1016/S1567-1348(02)00090-4[Crossref]
  • [5] Olopoenia LA, King AL. Widal agglutination test - 100 years later: still plagued by controversy. Postgrad Med J 2000;76:80–84. http://dx.doi.org/10.1136/pmj.76.892.80[Crossref]
  • [6] Tupasi TE, Lucas-Aquino R, Mendoza MT et al. Clinical Application of the Widal Test. Phil J Microbiol Infect Dis 1991; 20(1):23–26.
  • [7] World Health Organization. Background document: The diagnosis, treatment and prevention of typhoid fever. WHO/V & B / 2007; 03: 1–38.
  • [8] Gilman, R.H., Terminel, M., Levine, M.M. et al. Relative efficacy of blood, urine, rectal swab, bonemarrow, and rose-spot cultures for recovery of Salmonella typhi in typhoid fever. Lancet 1975; 1 1211–1213. http://dx.doi.org/10.1016/S0140-6736(75)92194-7[Crossref]
  • [9] Hoffman, S.L., Punjabi, N.H., Rockhill, R.C. et al. Duodenal string capsule culture compared with bone-marrow, blood and rectal cultures for diagnosis typhoid and a paratyhphoid fever. J. Infect. Dis. 1984:149: 157–161.
  • [10] Cocolin L., Manzano M., Astori G et al. A highly sensitive and fast nonradioactive method for the detection of polymerase chain reaction products from Salmonella serovars, such as Salmonella Typhi, in blood specimens. FEMS Immunol and MedicMicrobiol 1998; 22: 233–239. http://dx.doi.org/10.1111/j.1574-695X.1998.tb01211.x[Crossref]

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_s11536-008-0052-8
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