The study was undertaken to assess job satisfaction among health care workers (HCWs) who work with disabled patients and to identify the factors that contribute to their job satisfaction. This was a cross-sectional study conducted at nine rehabilitation centers in Serbia. The study sample consisted of doctors, nurses, physiotherapists, and other HCWs (social workers, work and speech therapists, and psychologists). A self-administrated questionnaire was distributed to 170 HCWs. Chi square testing and multivariate logistic regression analyses were applied. The response rate was 97.1% (165/170). Overall job satisfaction was reported by 22.4% of the respondents and was associated with hospital politics (odds ratio [OR], 4.16; 95% confidence interval [CI], 1.13–15.30], personal relationships (OR, 13.96; 95% CI, 4.07–47.92) and the feeling of being able to provide a good quality of care (OR, 5.24; 95% CI, 1.62–16.99). Profession, age, and gender did not affect job satisfaction. In conclusion, the results show very low job satisfaction among HCWs. Improving contributing factors may lead to higher job satisfaction and a better quality of health care.