Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings

• Authors: Ugur Ilga , Mustafa Ozyurt , Sukru Yildirim , Koray Ergunay , Nurittin Ardic , Levent Demirturk , Tuncer Haznedaroglu
• Languages of publication: EN

Abstracts

EN

Colonization with Helicobacter pylori (HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlations of histopathologic changes with bacterial virulence factors and serologic profiles were investigated. Gastric biopsies from sixty-nine patients admitted to Haydarpasa Training Hospital Department of Gastroenterology were evaluated for rapid urease, HP isolation and examined histopathologically. PCR was employed for HP confirmation and detection of HP cagA, cagE and virB11 genes. For each patient, IgG and IgA antibodies and anti-cagA antibodies were also determined by ELISA tests. HP was isolated and confirmed by PCR in 74% (51/69) of the patients. Anti-HP IgG and IgA were detected in 96% (49/51) and 53% (27/51), respectively. Anti-cagA were present in 51% (26/51). cagA, cagE and virB11 were positive in 56.8% (29/51), 60.7% (31/51) and 58.8% (30/51) of the patients, respectively. Statistically significant correlation was observed between cagA PCR and inflammation/activity scores. Detection of cagA by molecular assays can be an alternative test that can be employed for individual patient assessment.

Keywords

EN

Helicobacter pylori; Serology; Pathogenesis; Non-ulcer dyspepsia; CagA

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2008
• Volume: 3
• Issue: 1
• Pages: 41-46

Dates

published

1 - 3 - 2008

online

1 - 3 - 2008

Contributors

author

Ugur Ilga

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey

author

Mustafa Ozyurt

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey

author

Sukru Yildirim

• Haydarpasa Training Hospital, Department of Pathology, Gulhane Military Medical Academy, Istanbul, Turkey

author

Koray Ergunay

• Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, Ankara, Turkey

author

Nurittin Ardic

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey

author

Levent Demirturk

• Haydarpasa Training Hospital, Department of Gastroenterology, Gulhane Military Medical Academy, Istanbul, Turkey

author

Tuncer Haznedaroglu

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey

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Identifiers

DOI

10.2478/s11536-007-0062-y