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Journal

2006 | 1 | 3 | 250-260

Article title

Performance of three PCR methods targeting different regions of viral genome for the detection of TTV in Non A-E hepatitis, chronic B and C hepatitis and healthy blood donors

Content

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Languages of publication

EN

Abstracts

EN
TT virus (TTV) was suggested to be the etiologic agent for non A-E hepatitis but this could not yet be proven due to high detection rates not only in hepatitis but also in healthy persons and sensitivity differences of PCR methods employed. The aim of this study was to evaluate TTV DNA positivity in non A-E hepatitis cases, chronic HBV and HCV hepatitis cases and healthy blood donors via PCR systems that target all regions of the viral genome used for viral detection. 23 non A-E hepatitis, 28 chronic HCV, 21 chronic HBV cases and 56 healthy blood donors were included in the study and evaluated by PCR protocols that target 5′-UTR, 3′-UTR and N22 (ORF1) regions. As a result, 3′-UTR and 5′-UTR PCR had comparable detection rates that were higher than N22 PCR. Differences in detection rates among study groups were not statistically significant for any PCR method. Hepatic enzyme levels of the patients were not correlated with the presence of TTV DNA. Detection rate was significantly higher for Non A-E hepatitis group when positivity rates from all methods were combined. These results suggest an alteration of viral genotypes in Non A-E hepatitis which might be associated with pathogenesis.

Publisher

Journal

Year

Volume

1

Issue

3

Pages

250-260

Physical description

Dates

published
1 - 9 - 2006
online
1 - 9 - 2006

Contributors

author
  • Department of Clinical Microbiology, Virology Unit, Hacettepe University Faculty of Medicine, Ankara, Turkey
author
  • Department of Internal Medicine, Gastroenterology Division, Hacettepe University Faculty of Medicine, Ankara, Turkey
  • Department of Biostatistics, Hacettepe University Faculty of Medicine, Ankara, Turkey
  • Department of Clinical Microbiology, Virology Unit, Hacettepe University Faculty of Medicine, Ankara, Turkey
  • Department of Internal Medicine, Gastroenterology Division, Hacettepe University Faculty of Medicine, Ankara, Turkey

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_s11536-006-0029-4
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