Estimate of antioxidant capacity and lipid peroxidation in plasma of healthy subjects
Languages of publication
Serum contains various antioxidant molecules that may provide important protection against free radical attack. The aim of this work was to assess the total antioxidant capacity of plasma and a marker of lipid per oxidation [(thiobarbituric acid-reactive substances (TBARS)] in plasma of healthy smoking and non-smoking young and elderly subjects. In addition, we investigated plasma concentrations of α-tocopherol, β-carotene, and ascorbic acid. In in vitro experiments, the effects of exogenous compounds (ascorbic acid, uric acid, Trolox) on total ferric-reducing activity of plasma (FRAP) were also tested. We demonstrated that total antioxidant capacity of plasma obtained from healthy non-smoking young subjects was significantly higher than plasma antioxidant capacity of smoking elderly subjects. The concentration of TBARS in young non-smoking volunteers was lower than that in young smokers. The concentration of TBARS in elderly non-smoking volunteers was lower than in elderly smokers. Plasma concentrations of alpha-tocopherol, beta-carotene and ascorbic acid were significantly lower in elderly smoker than in elderly non-smokers of the same age. No difference in the plasma levels of alpha-tocopherol, beta-carotene and ascorbic acid were found in 22-year-old smoking and non-smoking subjects. In vitro addition of ascorbic acid, uric acid, or Trolox to plasma samples significantly increased their total antioxidant capacity. Decrease of FRAP values and increase of TBARS concentrations is a significant physiologic condition of the aging process. Supplementation of antioxidants could be useful for the enhancement of antioxidant screen in plasma.
1 - 3 - 2006
1 - 3 - 2006
- Chair of Experimental and Clinical Physiology, Department of Cardiovascular Physiology, Medical University, Lodz, Poland, firstname.lastname@example.org
- Chair of Experimental and Clinical Physiology, Department of Cardiovascular Physiology, Medical University, Lodz, Poland
-  B. Halliwell, J.M. Gutteridge and C.E. Cross: “Free radicals, antioxidants, and human disease: where are we now?”, Clin. Lab. Med., Vol. 119, (1992), pp. 598–620.
-  B.P. Yu: “Cellular defenses against damage from reactive oxygen species”, Physiol. Rev., Vol. 74, (1994), pp. 139–162.
-  C. Di Giacomo, R. Acquaviva, R. Lanteri, F. Licata, A. Licata and A. Venella: “Nonproteic antioxidant status in plasma of subjects with colon cancer”, Exp. Biol. Med., Vol. 228, (2003), pp. 525–528.
-  O. Öztürk and S. Gümüslu: “Changes in glucose-6 phosphate dehydrogenase, copper, zinc-superoxide dismutase and catalase activities, glutathione and its metabolizing enzymes, and lipid peroxidation in rat erythrocytes with age”, Exp. Gerontol., Vol. 39, (2004), pp. 211–216. http://dx.doi.org/10.1016/j.exger.2003.10.015[Crossref]
-  J. Lykkesfeldt, M. Viscovich and H.E. Poulsen: “Ascorbic acid recycling in human erythrocytes is induced by smoking in vivo”, Free Radic. Biol. Med., Vol. 35, (2003), pp. 1439–1447. http://dx.doi.org/10.1016/j.freeradbiomed.2003.08.006[Crossref]
-  D.R. Janero: “Malondialdehyde and thiobarbituric acid reactivity as diadnostic indices of lipid peroxidation and peroxidative tissue injury”, Free Radic. Biol. Med., Vol. 9, (1990), pp. 515–540. http://dx.doi.org/10.1016/0891-5849(90)90131-2[Crossref]
-  J. Lehotsky, P. Kaplan, P. Racay, M. Matejovicova, A. Drgova and V. Mezesova: “Membrane ion transport systems during oxidative stress in rodent brain: protective effect of stobadine and other antioxidants”, Life Sci., Vol. 65, (1999) pp. 1951–1958. http://dx.doi.org/10.1016/S0024-3205(99)00454-3[Crossref]
-  G.G. Duthie, J.R. Arthur, J.A. Beattie, K.M. Brown, P.C. Morrice, J.D. Robertson, C.T. Shortt, K.A. Walker and W.P. James: “Cigarette smoking, antioxidants, lipid peroxidation, and coronary heart disease”, Ann. N Y Acad. Sci., Vol. 686, (1993), pp. 120–129.
-  E. Hasnis and A.Z. Reznick: “Antioxidants and healthy aging”, Isr. Med. Assoc. J., Vol. 5, (2003) pp. 368–370.
-  B. Van Der Loo, M. Bachschmid, V. Spitzer, L. Brey, V. Ullrich and T.F. Luscher: “Decreased plasma and tissue levels of vitamin C in a rat model of aging: implications for antioxidative defense”, Biochem. Biophys. Res. Commun., Vol. 303, (2003), pp. 483–487. http://dx.doi.org/10.1016/S0006-291X(03)00360-7[Crossref]
-  I.F. Benzie and J.J. Strain: “The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”. The FRAP assay”, Anal. Bioch., Vol. 239, (1996), pp. 70–76. http://dx.doi.org/10.1006/abio.1996.0292[Crossref]
-  G. Cao and R.L. Prior: “Comparison of different analytical methods for assessing total antioxidant capacity of human serum”, Clin. Chem., Vol. 44, (1998), pp. 1309–1315.
-  M. Kasielski and D. Nowak: “Long-term administration of N-acetylcysteine decreases hydrogen peroxide exhalation in subjects with chronic obstructive pulmonary disease”, Respir. Med., Vol. 95, (2001) pp. 448–456. http://dx.doi.org/10.1053/rmed.2001.1066[Crossref]
-  B.L. Lee, S.C. Chua, H.Y. Ong and C.N. Ong: “High performance liquid chromatographic method for routine determination of vitamins A and E and beta-carotene in plasma”, J. Chromatogr., Vol. 581, (1992) pp. 41–47.
-  J. Bełtowski, G. Wójcicka, D. Górny and A. Marciniak: “The e.ect of dietary-induced obesity on lipid peroxidation, antioxidant enzymes and total plasma antioxidant capacity”, J. Physiol. Pharmacol., Vol. 51, (2000) pp. 883–896.
-  A. Ghiselli, M. Serafini, F. Natella and C. Scaccini: “Total antioxidant capacity as a tool to assess redox status: critical view and experimental data”, Free Radic. Biol. Med., Vol. 29, (2000) pp. 1106–1114. http://dx.doi.org/10.1016/S0891-5849(00)00394-4[Crossref]
-  C. Vassalle, S. Masini, C. Carpeggiani, A. L’Abbate, C. Boni and G.C. Zucchelli: “In vivo total antioxidant capacity: comparison of two different analytical methods”, Clin. Chem. Lab. Med., Vol. 42, (2004), pp. 84–89. http://dx.doi.org/10.1515/CCLM.2004.016[Crossref]
-  O. Erel: “A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation”, Clin. Biochem., Vol. 37, (2004), pp. 277–285. http://dx.doi.org/10.1016/j.clinbiochem.2003.11.015[Crossref]
-  J.J. Castenmiller, S.T. Lauridsen, L.O. Dragsted, K.H. van het Hof, J.P. Linssen and C.E. West: “Beta-carotene does not change markers of enzymatic and nonenzymatic antioxidant activity in human blood”, J. Nutr., Vol. 129, (1999), pp. 2162–2169.
-  H. Alho, J.S. Leinonen, M. Erhola, K. Lonnrot and R. Aejmelaeus: “Assay of antioxidant capacity of human plasma and CSF in aging and disease”, Restor. Neurol. Neurosci., Vol. 12, (1998), pp. 159–165.
-  U. Mutlu-Turkoglu, E. Ilhan, S. Oztezcan, A. Kuru, G. Aykac-Toker and M. Uysal: “Age-related increases in plasma malondialdehyde and protein carbonyl levels and lymphocyte DNA damage in elderly subjects”, Clin. Biochem., Vol. 36, (2003) pp. 397–400. http://dx.doi.org/10.1016/S0009-9120(03)00035-3[Crossref]
-  G. Bekesi, R. Kakucs, J. Sandor, Y.E. Sarvary, I. Kocsis, D. Sprintz, S. Varbiro, Z. Magyar, A. Hrabak, J. Feher and B. Szekacs: “Plasma concentration of myeloperoxidase enzyme in pre-and post-climacterial people: related superoxide anion generation”, Exp. Gerontol., Vol. 37, (2001), pp. 137–148. http://dx.doi.org/10.1016/S0531-5565(01)00151-6[Crossref]
-  J.F. Lesgards, P. Durand, M. Lassarre, P. Stocker, G. Lesgards, A. Lanteaume, M. Prost and M.P. Lehucher-Michel: “Assessment of lifestyle effects on the overall antioxidant capacity of healthy subjects”, Environ. Health Perspect., Vol. 110, (2002), pp. 479–486.
-  D.P. Jones, V.C. Mody Jr, J.L. Carlson, M.J. Lynn and P. Sternberg Jr: “Redox analysis of human plasma allows separation of pro-oxidant events of aging from decline in antioxidant defenses”, Free Radic. Biol. Med., Vol. 33, (2002), pp. 1290–1300. http://dx.doi.org/10.1016/S0891-5849(02)01040-7[Crossref]
-  E.M. Kukovetz, G. Bratschitsch, H.P. Hofer, G. Egger and R.J. Schaur: “Influence of age on the release of reactive oxygen species by phagocytes as measured by a whole blood chemiluminescence assay”, Free Radic. Biol. Med., Vol. 22, (1997), pp. 433–438. http://dx.doi.org/10.1016/S0891-5849(96)00334-6[Crossref]
-  W.A. Pryor and K. Stone: “Oxidants in cigarette smoke. Radicals, hydrogen peroxide, peroxynitrate, and peroxinitrite”, Ann. NY Anad. Sci., Vol. 686, (1993), pp. 12–27.
-  M. Serafini: “Dietary vitamin E and T cell-mediated function in the eldery: effectivenes and mechanism of action”, Int. J. Dev. Neurosci., Vol. 18, (2000), pp. 401–410. http://dx.doi.org/10.1016/S0736-5748(00)00016-2[Crossref]
-  B.I. Ognjanovic, S.Z. Pavlović, S.D. Maletić, R.V. Zikić, A.S. Stajn, R.M. Radojicić, Z.S. Saicić and V.M. Petrović: “Protective influence of vitamin E on antioxidant defense system in the blood of rats treated with cadmium”, Physiol. Res., Vol. 52, (2003) pp. 563–570.
-  N. Sumien, M.J. Forster and R.S. Sohal: “Supplementation with vitamin E fails to attenuate oxidative damage in aged mice”, Exp. Gerontol., Vol. 38, (2003), pp. 699–704. http://dx.doi.org/10.1016/S0531-5565(03)00068-8[Crossref]
-  A.J. Michels, N. Joisher and T.M. Hagen: “Age-related decline of sodium-dependent ascorbic acid transport in isolated rat hepatocytes”, Arch. Biochem. Biophys., Vol. 410, (2003) pp. 112–120. http://dx.doi.org/10.1016/S0003-9861(02)00678-1[Crossref]
-  F.J. Nieto, C. Iribarren, M.D. Gross, G.W. Comstock and R.G. Cutler: “Uric acid and serum antioxidant capacity: a reaction to atherosclerosis?”, Atheroscler., Vol. 148, (2000), pp. 131–139. http://dx.doi.org/10.1016/S0021-9150(99)00214-2[Crossref]
-  W.S. Waring, A. Convery, V. Mishra, A. Shenkin, D.J. Webb and S.R. Maxwell: “Uric acid reduces exercise-induced oxidative stress in healthy adults”, Clin Sci (Lond), Vol. 105, (2003), pp. 425–430. http://dx.doi.org/10.1042/CS20030149[Crossref]
-  C. Hawkins and M.J. Davies: “Hypochloride-induced damage to proteins: formation of nitrogen-centred radicals from lysine residues and their role in protein fragmentation”, Biochem. J., Vol. 332, (1998), pp. 617–625.
-  J. Mehlhorn, S. Sumida and L. Packer: “Tocopheroxyl radical persistence and tocopherol consumption in liposomes and in vitamin E-enriched rat liver mitochondria and microsomes”, J. Biol. Chem., Vol. 264, (1989), pp. 13448–13452.
-  A. Nagyova, M. Krajcovicova-Kudlackova, A. Horska, B. Smolkova, P. Blazicek, K. Raslova, A. Collins and M. Dusinska: “Lipid peroxidation in men after dietary supplementation with a mixture of antioxidant nutrients”. Bratisl. Lek. Listy., Vol. 105, (2004) pp. 277–280.
Publication order reference