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2006 | 1 | 1 | 46-57
Article title

Spatial visualisation and quantification of expression of cell specific markers - an application for chronic parathyroiditis cases

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EN
Abstracts
EN
The aim of this work was to develop a simple and fast technique for spatial visualisation of colour cell specific markers in digital microscopy. Colour light microscopy images were transformed into their spatial representation by introducing image brightness as the third dimension. Based on spatial representation, the expression of selected colour specific markers was assessed by their surface, volume, area fraction and colour intensity. This technique was implemented for quantitative analysis of expression of PCNA, Ki67 and bcl-2 in chronic parathyroiditis cases. Comparing to standard, colour sampling techniques, the presented method based on spatial visualisation of the expression of markers in tissue sections, seems an effective and practical application in objective assessment of their distribution in the investigated material.
Publisher

Journal
Year
Volume
1
Issue
1
Pages
46-57
Physical description
Dates
published
1 - 3 - 2006
online
1 - 3 - 2006
Contributors
  • Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland, elka@amp.edu.pl
  • Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland
  • Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland
  • Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11536-006-0005-z
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