Spatial visualisation and quantification of expression of cell specific markers - an application for chronic parathyroiditis cases

• Authors: Elżbieta Kaczmarek , Rafał Strzelczyk , Agnieszka Górna , Przemysław Majewski
• Languages of publication: EN

Abstracts

EN

The aim of this work was to develop a simple and fast technique for spatial visualisation of colour cell specific markers in digital microscopy. Colour light microscopy images were transformed into their spatial representation by introducing image brightness as the third dimension. Based on spatial representation, the expression of selected colour specific markers was assessed by their surface, volume, area fraction and colour intensity. This technique was implemented for quantitative analysis of expression of PCNA, Ki67 and bcl-2 in chronic parathyroiditis cases. Comparing to standard, colour sampling techniques, the presented method based on spatial visualisation of the expression of markers in tissue sections, seems an effective and practical application in objective assessment of their distribution in the investigated material.

Keywords

EN

Visualisation of immunohistochemical markers; spatial colour segmentation; proliferation markers; apoptosis; chronic parathyroiditis

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2006
• Volume: 1
• Issue: 1
• Pages: 46-57

Dates

published

1 - 3 - 2006

online

1 - 3 - 2006

Contributors

author

Elżbieta Kaczmarek

• Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland

author

Rafał Strzelczyk

• Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland

author

Agnieszka Górna

• Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland

author

Przemysław Majewski

• Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences, Poznań, Poland

References

• [1] R.A. Robb: “Three-Dimensional Visualization in Medicine and Biology”, In: Handbook of Medical Imaging. Processing and Analysis, Academic Press, San Diego, 2000, pp. 685–712.
• [2] E. Nieruchalska, R. Strzelczyk, A. Wozniak, J. Zurawski, E. Kaczmarek and W. Salwa-Zurawska: “A quantitative analysis of the expression of α-smooth muscle action in mesangioproliferative (GnMes) glomerulonephritis”, Folia Morph., Vol. 62, (2003), pp. 451–453.
• [3] J.C. Russ: The Image Processing Handbook, CRC Press, Ann Arbor, 1995.
• [4] R.S. Wright Jr and M. Sweet: OpenGL superbible: the complete guide to OpenGL programming for Windows NT and Windows 95, Waite Group Press, 1996.
• [5] K. Matkowskyj, D. Schonfeld and R. Benya: “Quantitative Immunohistochemistry by Measuring Cumulative Signal Strength Using Commercially Available Software Photoshop and Matlab”, J. Histochem. Cytochem., Vol. 48, (2000), pp. 163–174.
• [6] K.A. Matkowskyj, R. Cox, R.T. Jensen and R. Benya: “Quantitative Immunohistochemistry by Measuring Cumulative Signal Strength Accurately Measures Receptor Number”, J. Histochem. Cytochem., Vol. 51, (2003), pp. 205–214. [Crossref]
• [7] E. Kaczmarek, A. Górna and P. Majewski: “Techniques of image analysis for quantitative immunohistochemistry”, Ann. Acad. Med. Bialostocen., Vol. 49(Suppl 1), (2004), pp. 155–158.
• [8] W. Remmele and H.E. Stegner: “Vorschlag zur einheitlichen Definition eines immunoreactiven Score (IRS) fur den Immunohistochemichen Ostrogenrezeptor - Nachweis (ER-ICA) im Mammakarzinomgewebe”, Pathologe, Vol. 8, (1987), pp. 138–140.
• [9] L.J. Hammond, M.W. Lowdell, P.G. Cerrano, A.W. Goode, G.F. Bottazzo and R. Mirakian: “Analysis of apoptosis in relation to tissue destruction associated with Hashimoto’s autoimmune thyroiditis”, J. Pathol., Vol. 182, (1997), pp. 138–144. http://dx.doi.org/10.1002/(SICI)1096-9896(199706)182:2<138::AID-PATH810>3.0.CO;2-F[Crossref]
• [10] M. Cozzolino, Y. Lu, J. Finch, E. Slatopolsky and A.S. Dusso: “p21WAF1 and TGFalpha mediate parathyroid growth arrest by vitamin D and high calcium”, Kidney Int., Vol. 60, (2001) pp. 2109–2117. http://dx.doi.org/10.1046/j.1523-1755.2001.00042.x[Crossref]
• [11] L.A. Erickson, L. Jin, M. Papotti and R.V. Lloyd: “Oxyphil parathyroid carcinomas: a clinicopathologic and immunohistochemical study of 10 cases”, Am. J. Surg. Pathol., Vol. 26, (2002), pp. 344–349. http://dx.doi.org/10.1097/00000478-200203000-00008[Crossref]

Identifiers

DOI

10.2478/s11536-006-0005-z