Changes of the expression of insulin-like growth factor 1 and its two receptors in the periphery of the developing lung are parallel to ultrastructural capillary changes during perinatal period

• Authors: Andrzej Marszałek , Wiesława Biczysko , Tsutomu Daa , Elżbieta Kaczmarek , Marcin Wąsowicz , Shigeo Yokoyama
• Languages of publication: EN

Abstracts

EN

Insulin-like growth factor type 1 (IGF-1) belongs to the peptide hormones which regulate cell proliferation and differentiation as well as angiogenesis.The aim of the present study was the quantitative evaluation of immuohistochemical (IH) expression of IGF-1 and its two receptors (IGF-1Rα and IGF-1Rβ) parallel to changes of capillary lumen/whole capillary area (L/W) ratio during the perinatal period.Tissue samples from lungs from 19-, and 21-day fetuses as well as 1-, 3-, 5-day old newborns were processed for frozen sections that were used for IH staining and then used for quantitative analysis of antigens expression. In parallel, tissue samples were processed for transmission electron microscopy and used for capillaries morphometry. Our results were statistically evaluated.We found that the expression of IGF-1 in the peripheral part of the developing lung has a peak at the moment of birth when changes of vascular structure were reflected by L/W ratio. This is followed by the peak expression of the IGF-1Rα, but not of the IGF-1Rβ.

Keywords

EN

Insulin-like growth factor-1 (IGF-1); morphometry; electron microscopy; experimental study; perinatal period

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2006
• Volume: 1
• Issue: 1
• Pages: 2-11

Dates

published

1 - 3 - 2006

online

1 - 3 - 2006

Contributors

author

Andrzej Marszałek

• Chair and Department of Clinical Pathomorphology, Poznan University of Medical Sciences, 60-355, Poznan, Poland

author

Wiesława Biczysko

• Chair and Department of Clinical Pathomorphology, Poznan University of Medical Sciences, 60-355, Poznan, Poland

author

Tsutomu Daa

• Department of Pathology, Oita Medical University, Oita, Japan

author

Elżbieta Kaczmarek

• Chair and Department of Clinical Pathomorphology, Poznan University of Medical Sciences, 60-355, Poznan, Poland

author

Marcin Wąsowicz

• Intensive Care Unit, Collegium Medicum Jagiellonian University, Kraków, Poland

author

Shigeo Yokoyama

• Department of Pathology, Oita Medical University, Oita, Japan

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Identifiers

DOI

10.2478/s11536-006-0003-1