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Journal
2011 | 9 | 2 | 293-299
Article title

SIL-based confocal fluorescence microscope for investigating individual nanostructures

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Languages of publication
EN
Abstracts
EN
We developed a fluorescence confocal microscope equipped with a hemispherical solid immersion lens (SIL) and apply it to study the optical properties of light-harvesting complexes. We demonstrate that the collection efficiency of the SIL-equipped microscope is significantly improved, as is the spatial resolution, which reaches 600 nm. This experimental setup is suitable for detailed studies of physical phenomena in hybrid nanostructures. In particular, we compare the results of fluorescence intensity measurements for a light-harvesting peridinin-chlorophyll-protein (PCP) complex with and without the SIL.
Publisher
Journal
Year
Volume
9
Issue
2
Pages
293-299
Physical description
Dates
published
1 - 4 - 2011
online
20 - 2 - 2011
References
  • [1] A.M. van Oijen, M. Ketelaars, J. Köhler, T.J. Aartsma, J. Schmidt, Science 285, 400 (1999)
  • [2] Q. Wu, G.D. Feke, R.D. Grober, L.P. Ghislain, Appl. Phys. Lett. 75, 4064 (1999) http://dx.doi.org/10.1063/1.125537[Crossref]
  • [3] S. Moehl, H. Zhao, B. Dal Don, S. Wachter, H. Kalt, J. Appl. Phys. 93, 6265 (2003) http://dx.doi.org/10.1063/1.1567035[Crossref]
  • [4] M. Baba, T. Sasaki, M. Yoshita, H. Akiyama, J. Appl. Phys. 85, 6923 (1999) http://dx.doi.org/10.1063/1.370107[Crossref]
  • [5] K.P. Hewaparakrama et al., Appl. Phys. Lett. 85, 5463 (2004) http://dx.doi.org/10.1063/1.1829169[Crossref]
  • [6] E. Hofmann et al., Science 272, 1788 (1996) http://dx.doi.org/10.1126/science.272.5269.1788[Crossref]
  • [7] D.J. Miller et al., Photosynth. Res. 86, 229 (2005) http://dx.doi.org/10.1007/s11120-005-2067-1[Crossref]
  • [8] T. Schulte et al., P. Natl. Acad. Sci. USA 106, 20764 (2009) http://dx.doi.org/10.1073/pnas.0908938106[Crossref]
  • [9] F.J. Kleima et al., Biophys. J. 78, 344 (2000) http://dx.doi.org/10.1016/S0006-3495(00)76597-0[Crossref]
  • [10] S. Mackowski et al., Nano Lett. 8, 558 (2008) http://dx.doi.org/10.1021/nl072854o[Crossref]
  • [11] S. Mackowski, J. Phys. Condens. Matter 22, 193102 (2010) http://dx.doi.org/10.1088/0953-8984/22/19/193102[Crossref]
  • [12] S. Wörmke et al., J. Fluoresc. 18, 611 (2008) http://dx.doi.org/10.1007/s10895-008-0310-9[Crossref]
  • [13] S. Wörmke et al., BBA-Bioenergetics 1767, 956 (2007) http://dx.doi.org/10.1016/j.bbabio.2007.05.004[Crossref]
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11534-010-0098-5
Identifiers
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