Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection

• Authors: Sylwia Magiera , Weronika Adolf , Irena Baranowska
• Languages of publication: EN

Abstracts

EN

A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng mL−1 to 200 ng mL−1 for the analysed compounds. The limit of quantification varied from 14.2 ng mL−1 to 24.2 ng mL−1. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. [...]

Keywords

EN

Chiral separation; HPLC; Method validation; Carvedilol enantiomers; 5′-Hyroxyphenyl carvedilol enantiomers

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 12
• Pages: 2076-2087

Dates

published

1 - 12 - 2013

online

26 - 9 - 2013

Contributors

author

Sylwia Magiera

• Department of Analytical Chemistry, Silesian University of Technology, 44-100, Gliwice, Poland

author

Weronika Adolf

• Department of Analytical Chemistry, Silesian University of Technology, 44-100, Gliwice, Poland

author

Irena Baranowska

• Department of Analytical Chemistry, Silesian University of Technology, 44-100, Gliwice, Poland

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Identifiers

DOI

10.2478/s11532-013-0329-x