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Journal
2013 | 11 | 11 | 1740-1748
Article title

Self-assembly of acetylcholinesterase on gold nanoparticles electrodeposited on graphite

Content
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EN
Abstracts
EN
The immobilisation of AChE enzyme through chemisorption on Au-modified graphite was examined with view of its prospective application in the design of membraneless electrochemical biosensors for the assay of enzyme inhibitors. The developed immobilisation protocol has been based on a two-stage procedure, comprising i) electrodeposition of gold nanostructures on spectroscopic graphite; followed by ii) chemisorption of the enzyme onto gold nanoparticles. Both the coverage of the electrode surface with Au nanostructures and the conditions for enzyme immobilisation were optimised. The proposed electrode architecture together with the specific type of enzyme immobilisation allow for a long-term retaining of the enzyme catalytic activity. The extent of inhibition of the immobilised acetylcholinesterase enzyme by the organophosphorous compound monocrotophos has been found to depend linearly on its concentration over the range from 50 to 400 nmol mL−1 with sensitivity 77.2% inhibition per 1 µmol mL−1 of monocrotophos. [...]
Publisher

Journal
Year
Volume
11
Issue
11
Pages
1740-1748
Physical description
Dates
published
1 - 11 - 2013
online
25 - 8 - 2013
Contributors
author
  • Department Physical Chemistry, Plovdiv University, BG-4000, Plovdiv, Bulgaria
author
  • Department Biotechnology, University “Prof. Dr Assen Zlatarov”, BG - 8010, Burgas, Bulgaria
  • Department Biotechnology, University “Prof. Dr Assen Zlatarov”, BG - 8010, Burgas, Bulgaria, godjevargova@yahoo.com
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-013-0307-3
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