A simple TLC and HPTLC method for separation of selected steroid drugs

• Authors: Joanna Nowakowska , Piotr Pikul , Krzesimir Ciura , Jakub Piotrowicz
• Languages of publication: EN

Abstracts

EN

Chromatographic properties of five steroid drugs: cortisone, hydrocortisone, methylprednisolone, prednisolone and norgestrel have been studied by normal-, reversed-phase and hydrophilic neutral cyano-bonded silica stationary phase with five binary mobile phases (acetonitrile-water, acetonitrile-DMSO, acetonitrile-methanol, acetone-petroleum ether, acetone-water) in which the concentration of organic modifier was varied from 0 to 100% (v/v). This study reports the optimization of steroid hormones separation. Chromatographic retention data and possible retention mechanisms are discussed. Separation abilities of mobile and stationary phases were studied using the principal component analysis method. The best separation of methylprednisolone and prednisolone is with a chromatographic system included silica gel as stationary phase and mixture of acetonitrile and DMSO (10:90 v/v). These two anti-inflammatory drugs can be fast separated from norgestrel when CN is used as stationary phase and acetone and water (40:60 v/v) as mobile phase. The highest values of the parameter Δ(ΔG°) and alfa for cortisone and hydrocortisone was observed in case of using CN as stationary phase and water-acetonitryle (40:60 v/v) as mobile phase. [...]

Keywords

EN

Principal component analysis; Separation factor; Standard (Gibbs) free energy change of partition; Steroid drugs; Thin-layer chromatography

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 8
• Pages: 1297-1308

Dates

published

1 - 8 - 2013

online

1 - 6 - 2013

Contributors

author

Joanna Nowakowska

• Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 80-416, Gdańsk, Poland

author

Piotr Pikul

• Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 80-416, Gdańsk, Poland

author

Krzesimir Ciura

• Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 80-416, Gdańsk, Poland

author

Jakub Piotrowicz

• Department of Economics and Law, Maritime Institute in Gdańsk, 80-830, Gdańsk, Poland

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Identifiers

DOI

10.2478/s11532-013-0258-8