Development of a nanocomposite system and its application in biosensors construction

• Authors: Ramona Penu , Ioana Vasilescu , Sandra Eremia , Florentina Gatea , Gabriel-Lucian Radu , Simona-Carmen Litescu
• Languages of publication: EN

Abstracts

EN

The present work describes the development of a nanocomposite system and its application in construction of a new amperometric biosensor applied in the determination of total polyphenolic content from propolis extracts. The nanocomposite system was based on covalent immobilization of laccase on functionalized indium tin oxide nanoparticles and it was morphologically and structural characterized. The casting of the developed nanocomposite system on the surface of a screen-printed electrode was used for biosensor fabrication. The analytical performance characteristics of the settled biosensor were determined for rosmarinic acid, caffeic acid and catechol (as laccase specific substrate). The linearity was obtained in the range of 1.06×10−6 − 1.50×10−5 mol L−1 for rosmarinic acid, 1.90×10−7 − 2.80×10−6 mol L−1 for caffeic acid and 1.66×10−6 − 7.00×10−6 mol L−1 for catechol. A good sensitivity of amperometric biosensor 141.15 nA µmol−1 L−1 and fair detection limit 7.08×10−8 mol L−1 were obtained for caffeic acid. The results obtained for polyphenolic content of propolis extracts were compared with the chromatographic data obtained by liquid-chromatography with diode array detection. [...]

Keywords

EN

Nanocomposite; Laccase biosensor; Polyphenols; Propolis extracts; Indium tin oxide nanoparticles

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 6
• Pages: 968-978

Dates

published

1 - 6 - 2013

online

28 - 3 - 2013

Contributors

author

Ramona Penu

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

author

Ioana Vasilescu

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

author

Sandra Eremia

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

author

Florentina Gatea

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

author

Gabriel-Lucian Radu

• Faculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, Bucharest, 0101061, Romania

author

Simona-Carmen Litescu

• National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, Bucharest, 060031, Romania

References

• [1] X. Feng, R. Li, Y. Ma, Q. Fan, W. Huang, Synthetic Met. 161, 1940 (2011) http://dx.doi.org/10.1016/j.synthmet.2011.06.039[Crossref]
• [2] Z. Gao, Y.Z. Gao, Y.H. Li, L. Yi, Nanostruct. Mater. 11, 611 (1999)
• [3] C. Goebbert, R. Nonninger, M.A. Aegerter, H. Scmidt, Thin Solid Films 351, 79 (1999)
• [4] A. Radoi, A. Iordanescu, A. Cismaru, M. Dragoman, D. Dragoman, Nanotechnology 21, 455202 (2010) http://dx.doi.org/10.1088/0957-4484/21/45/455202[Crossref]
• [5] V. Senthilkumar, K. Senthil, P. Vickraman, Mater. Res. Bull. 47, 1051 (2012) http://dx.doi.org/10.1016/j.materresbull.2011.12.040[Crossref]
• [6] A.I. Yaropolov, O.V. Skorobogat’ko, S.S. Vartanov, S.D. Varfolomeyer, Appl. Biochem. Biotech. 49, 257 (1994) [Crossref]
• [7] C.F. Thurston, Microbiology 140, 19 (1994) http://dx.doi.org/10.1099/13500872-140-1-19[Crossref]
• [8] P. V. Bernhard, Aust. J. Chem. 59, 233 (2006) http://dx.doi.org/10.1071/CH05340[Crossref]
• [9] P. J. Collins, M. Kotterman, J. A. Field, A. Dobson, Appl. Environ. Microbiol. 62, 4563 (1996)
• [10] M. Y. Ahn, J. Dec, J. E. Kim, J. M. Bollag, J. Environ. Qual. 31, 1509 (2002) http://dx.doi.org/10.2134/jeq2002.1509[Crossref]
• [11] F. Vianello, S. Ragusa, M.T. Cambria, A. Rigo, Biosens. Bioelectron. 21, 2155 (2006) http://dx.doi.org/10.1016/j.bios.2005.10.006[Crossref]
• [12] S.A.S.S Gomes, M.J.F. Rebelo, Sensors 3, 166 (2003) http://dx.doi.org/10.3390/s30600166[Crossref]
• [13] A. E. Gardiol, R. J. Hernandez, B. Reinhammar, B. R. Harte, Enz. Microb. Technol. 18, 347 (1996)
• [14] D. Leech, F. Daigle, Analyst 123, 19714 (1998)
• [15] C.A. Rice-Evans, N.J. Miller, G. Paganga, Trends Plant Sci. 2, 152 (1997) [Crossref]
• [16] A. Salvi, C. Brihlmann, E. Migliavacca, P.A. Carrupt, K. Hostettmann, B. Testa, Helv. Chim. Acta 85 (2002)
• [17] M. Antolovich, P.D. Prenzler, E. Patsalides, S. McDonald, K. Robards, Analyst, 127, 183 (2002) http://dx.doi.org/10.1039/b009171p[Crossref]
• [18] V.S. Bankova, S.L. Castro, M.C. Marcucci, Apidologie 31, 3 (2000) http://dx.doi.org/10.1051/apido:2000102[Crossref]
• [19] J.B. Daleprane, V. da Silva Freitas, A. Pacheco, M. Rudnicki, L.A. Faine, F.A. Dörr, M. Ikegaki, L.A. Salazar, T.P. Ong, D.S. Abdalla, J. Nutr. Biochem. 23, 557 (2012) http://dx.doi.org/10.1016/j.jnutbio.2011.02.012[Crossref]
• [20] E.W. Teixeira, G. Negri, R.M.S.A. Meira, D. Message, A. Salatino, Evidence Based Complementary Alternative Medicine 2, 85 (2005) http://dx.doi.org/10.1093/ecam/neh055[Crossref]
• [21] C. Castaldo, F. Capasso, Fitoterapia 73, 1 (2002) http://dx.doi.org/10.1016/S0367-326X(02)00185-5[Crossref]
• [22] M. González, B. Guzmán, R. Rudyk, E. Romano, M.A.A. Molina, Lat. Am. J. Pharm. 22, 243 (2003)
• [23] A.C.H.F. Sawaya, I.B. da Silva Cunha, M.C Marcucci, Chem. Cent. J. 5, 27 (2011) http://dx.doi.org/10.1186/1752-153X-5-27[Crossref]
• [24] S.A.S.S. Gomes, J.M.F. Nogueira, M.J.F. Rebelo, Biosens. Bioelectron. 20, 1211 (2004) http://dx.doi.org/10.1016/j.bios.2004.05.013[Crossref]
• [25] D.R. Theâvenot, K. Toth, R.A. Durst, G.S. Wilson, Pure Appl. Chem. 71, 2333 (1999)
• [26] Y. Pang, Z. Guang-Ming, T. Lin, Z. Yi, Li Zhen, C. Li-Juan, J. Cent. South Univ. Technol. 18, 1849 (2011) http://dx.doi.org/10.1007/s11771-011-0913-1[Crossref]
• [27] R.S. Freire, N. Durán, L.T. Kubota, Talanta 54, 681 (2001) http://dx.doi.org/10.1016/S0039-9140(01)00318-6[Crossref]
• [28] J. Kulys, R. Vidziunaite, Biosens Bioelectron. 18, 319 (2003) http://dx.doi.org/10.1016/S0956-5663(02)00172-0[Crossref]
• [29] G. Gupta, V. Rajendran, P. Atanassov, Electroanalysis 15, 1577 (2003) http://dx.doi.org/10.1002/elan.200302724[Crossref]
• [30] D. Quan, Y. Kim, W. Shin, J. Electroanal. Chem. 561, 181 (2004) http://dx.doi.org/10.1016/j.jelechem.2003.08.003[Crossref]
• [31] J. Zou, S.M. Kauzlarich, J. Clust. Sci., 19, 341 (2008) http://dx.doi.org/10.1007/s10876-008-0182-9[Crossref]
• [32] J.W. Schoonen, M.G. Sales, Anal. Bioanal. Chem. 372, 822 (2002) http://dx.doi.org/10.1007/s00216-002-1267-1[Crossref]
• [33] C.L. Nistor, D. Donescu, R. Ianchis, C. Spataru, V. Raditoiu, C. Petcu, M. Ghiurea, C. Deleanu, J. Sol-Gel Sci.Techn 59, 48 (2011) http://dx.doi.org/10.1007/s10971-011-2461-4[Crossref]
• [34] E. Teodor, S.C. Liţescu, C. Petcu, M. Mihalache, R. Somoghi, Nanoscale Res. Lett. 4, 544 (2009) http://dx.doi.org/10.1007/s11671-009-9278-x[Crossref]
• [35] M. Diaconu, A. Tache, S.A.V. Eremia, F. Gatea, S. Litescu, G.L. Radu, U.P.B. Sci. Bull. 72, 115 (2010)
• [36] H. Yi, K. Wu, S. Hu, D. Cui, Talanta 55, 1205 (2001) http://dx.doi.org/10.1016/S0039-9140(01)00531-8[Crossref]
• [37] A. Jarosz-Wilkołazka, T. Ruzgas, L. Gorton, Talanta 66, 1219 (2005) http://dx.doi.org/10.1016/j.talanta.2005.01.026[Crossref]
• [38] M. ElKaoutit, I. Naranjo-Rodriguez, K.R. Temsamani, M.P. Hernández-Artiga, D. Bellido-Milla, J.L.H.H.D. Cisneros, Food Chem. 110, 1019 (2008) http://dx.doi.org/10.1016/j.foodchem.2008.03.006[Crossref]
• [39] G. Socrates, Infrared and Raman Characteristic Group Frequencies, 3rd edition (John Wiley & Sons Ltd, Cichester, England, 2001) 328
• [40] S.K. Arya, A.K. Prusty, S.P. Singh, P.R. Solanki, M.K. Pandey, M. Datta, B.D. Malhotra, Anal. Biochem. 363, 210 (2007) http://dx.doi.org/10.1016/j.ab.2007.01.029[Crossref]
• [41] M. Lorenzo, D. Moldes, S. Rodríguez Couto, M.A. Sanromán, Chemosphere, 60, 1124 (2005) http://dx.doi.org/10.1016/j.chemosphere.2004.12.051[Crossref]
• [42] Y.Z. Xiao, X.M. Tu, J. Wang, M. Zhang, Q. Cheng, W.Y. Zeng, Y.Y. Shi, Appl. Microbiol. Biotechnol. 60, 700 (2003) [Crossref]
• [43] V.C. Sanz, M.L. Mena, A. Gonzalez-Cortes, P. Yanez-Sedeno, J.M. Pingarron, Anal.Chim. Acta 528, 1 (2005) http://dx.doi.org/10.1016/j.aca.2004.10.007[Crossref]
• [44] M. Diaconu, S.C. Litescu, G.L. Radu, Sensors and Actuators B, 145, 800 (2010) http://dx.doi.org/10.1016/j.snb.2010.01.064[Crossref]
• [45] P. Ibarra-Escutia, J.J. Gomez, C. Calas-Blanchard, J.L. Marty, M.T. Ramirez-Silva, Talanta 81, 1636 (2010) http://dx.doi.org/10.1016/j.talanta.2010.03.017[Crossref]
• [46] M. Diaconu, S.C. Litescu, G.L. Radu, Microchim. Acta 172, 177 (2011) http://dx.doi.org/10.1007/s00604-010-0486-y[Crossref]
• [47] S.C. Litescu, S.A.V. Eremia, A. Bertoli, L. Pistelli, G.L. Radu, Anal. Lett. 43, 1089 (2010) http://dx.doi.org/10.1080/00032710903518518[Crossref]
• [48] A.C.H.F. Sawaya, I.B.S. Cunha, M.C Marcucci, Chem. Cent. J. 5, 27 (2011) http://dx.doi.org/10.1186/1752-153X-5-27[Crossref]
• [49] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Free Rad. Biol. Med. 26, 1231 (1999) http://dx.doi.org/10.1016/S0891-5849(98)00315-3[Crossref]
• [50] G. Litwinienko, K.U. Ingold, J. Org. Chem. 69, 5888 (2004) http://dx.doi.org/10.1021/jo049254j[Crossref]

Identifiers

DOI

10.2478/s11532-013-0222-7