Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract

• Authors: Dragoljub Miladinović , Budimir Ilić , Stevo Najman , Olga Cvetković , Aleksandra Šajnović , Marija Miladinović , Nikola Nikolić
• Languages of publication: EN

Abstracts

EN

The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress. [...]

Keywords

EN

Astragalus onobrychis; Antioxidant enzymes; Reactive oxygen species; Antioxidant capacity; Chemiluminescence

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 2
• Pages: 123-132

Dates

published

1 - 2 - 2013

online

29 - 11 - 2012

Contributors

author

Dragoljub Miladinović

• Department of Pharmacy, Faculty of Medicine, University of Niš, 18000, Niš, Serbia

author

Budimir Ilić

• Department of Pharmacy, Faculty of Medicine, University of Niš, 18000, Niš, Serbia

author

Stevo Najman

• Department of Biology, Faculty of Medicine, University of Niš, 18000, Niš, Serbia

author

Olga Cvetković

• Center of Chemistry - IChTM, University of Belgrade, Njegoševa, 12, 11000, Belgrade, Serbia

author

Aleksandra Šajnović

• Center of Chemistry - IChTM, University of Belgrade, Njegoševa, 12, 11000, Belgrade, Serbia

author

Marija Miladinović

• Department of Pharmacy, Faculty of Medicine, University of Niš, 18000, Niš, Serbia

author

Nikola Nikolić

• Laboratory for Geochemistry, Cosmochemistry and Astrochemistry, University of Niš, 18000, Niš, Serbia

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Identifiers

DOI

10.2478/s11532-012-0163-6