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2013 | 11 | 2 | 123-132
Article title

Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract

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EN
Abstracts
EN
The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress. [...]
Publisher

Journal
Year
Volume
11
Issue
2
Pages
123-132
Physical description
Dates
published
1 - 2 - 2013
online
29 - 11 - 2012
Contributors
  • Department of Pharmacy, Faculty of Medicine, University of Niš, 18000, Niš, Serbia, dragoljubm@gmail.com
author
  • Department of Pharmacy, Faculty of Medicine, University of Niš, 18000, Niš, Serbia
author
  • Department of Biology, Faculty of Medicine, University of Niš, 18000, Niš, Serbia
  • Center of Chemistry - IChTM, University of Belgrade, Njegoševa, 12, 11000, Belgrade, Serbia
  • Center of Chemistry - IChTM, University of Belgrade, Njegoševa, 12, 11000, Belgrade, Serbia
  • Department of Pharmacy, Faculty of Medicine, University of Niš, 18000, Niš, Serbia
  • Laboratory for Geochemistry, Cosmochemistry and Astrochemistry, University of Niš, 18000, Niš, Serbia
References
  • [1] A. Pitzschke, C. Forzani, H. Hirt, Antioxid. Redox Sign. 8, 1757 (2006) http://dx.doi.org/10.1089/ars.2006.8.1757[Crossref]
  • [2] O. Ozkur, F. Ozdemir, M. Bor, I. Turkan, Environ. Exp. Bot. 66, 487 (2009) http://dx.doi.org/10.1016/j.envexpbot.2009.04.003[Crossref]
  • [3] Z. Noreen, M. Ashraf, Environ. Exp. Bot. 67, 395 (2009) http://dx.doi.org/10.1016/j.envexpbot.2009.05.011[Crossref]
  • [4] B. Márquez-García, F. Córdoba, Environ. Exp. Bot. 68, 58 (2010) http://dx.doi.org/10.1016/j.envexpbot.2009.10.008[Crossref]
  • [5] D. Podlech, Proc. Roy. Soc. Edinb. B. 89, 37 (1986)
  • [6] S.D. Lindblom et al., Environ. Exp. Bot. (in press), DOI:10.1016/j.envexpbot.2011.12.011 [Crossref]
  • [7] N. Diklić, In: M. Josifović (Ed.), Flora of Serbia (Serbian academy of Science and Art, Belgrade, 1974) 274
  • [8] D.L. Miladinović, B.S. Ilić, V.N. Milosavljević, Hem. Ind. 65, 323 (2011) http://dx.doi.org/10.2298/HEMIND110110019M[Crossref]
  • [9] A. Kabata-Pendias, Trace elements in soils and plants, 4th edition (CRC Press, New York, 2011)
  • [10] Z.A. Placer, L.L. Cushman, B.C. Johnson, Anal. Biochem. 16, 359 (1966) http://dx.doi.org/10.1016/0003-2697(66)90167-9[Crossref]
  • [11] H.P. Misra, I. Fridovich, J. Biol. Chem. 247, 3170 (1972)
  • [12] K.H. Cheeseman, A. Beavis, H. Esterbauer, Biochem. J. 252, 649 (1988)
  • [13] L.M. Simon, Z. Fatrai, D.E. Jonas, B. Matkovics, Biochem. Physiol. Pflanzen 166, 387 (1974)
  • [14] B. Matkovics, R. Novak, H.D. Hanh, Comp. Biochem. Phys. 56, 397 (1977)
  • [15] D.T.Y. Chiu, F.H. Stults, A.L. Tappel, Biochim. Biophys. Acta 445, 558 (1976) http://dx.doi.org/10.1016/0005-2744(76)90110-8[Crossref]
  • [16] M.M. Bradford, Anal. Biochem. 72, 248 (1976) http://dx.doi.org/10.1016/0003-2697(76)90527-3[Crossref]
  • [17] I. Jantan, N. Hikmah Harun, A. Wira Septama, S. Murad, M.A. Mesaik, J. Nat. Med. 65, 400 (2011) http://dx.doi.org/10.1007/s11418-010-0492-8[Crossref]
  • [18] W.S. Koko, M.A. Mesaik, S. Yousaf, M. Galal, M.I. Choudhary, J. Ethnopharmacol. 118, 26 (2008) http://dx.doi.org/10.1016/j.jep.2008.03.007[Crossref]
  • [19] V. Katsares et al., Lab. Med. 40, 557 (2009) http://dx.doi.org/10.1309/LMLE8BVHYWCT82CL[Crossref]
  • [20] D. Miladinović, Antioxidant system of plants (University of Niš, Niš, 2011)
  • [21] S. Najman, Č. Radojičić, A. Dujić, Period. Biol. 88, 256 (1986)
  • [22] R. Khanna-Chopra, D.S. Selote, Environ. Exp. Bot. 60, 276 (2007) http://dx.doi.org/10.1016/j.envexpbot.2006.11.004[Crossref]
  • [23] Y. Liang et al., Environ. Exp. Bot. 64, 286 (2008) http://dx.doi.org/10.1016/j.envexpbot.2008.06.005[Crossref]
  • [24] D. Miladinović, L. Miladinović, S. Najman, J. Serb. Chem. Soc. 76, 505 (2011) http://dx.doi.org/10.2298/JSC100701045M[Crossref]
  • [25] L. Simova-Stoilova, K. Demirevska, T. Petrova, N. Tsenov, U. Feller, Plant Soil Environ. 54, 529 (2008) [Crossref]
  • [26] P.A. Leand, MSc thesis, Texas Tech University (Lubbock, USA, 1986)
  • [27] J. Bogdanović, N. Milosavić, R. Prodanović, T. Dučić, K. Radotić, Biochem. Syst. Ecol. 35, 263 (2007) http://dx.doi.org/10.1016/j.bse.2006.12.001[Crossref]
  • [28] I. Öncel, E. Yurdakulol, Y. Keleş, L. Kurt, A. Yildiz, Acta Oecol. 26, 211 (2004) http://dx.doi.org/10.1016/j.actao.2004.04.004[Crossref]
  • [29] J.V. Anderson, B.I. Chevone, J.L. Hess, Plant Physiol. 98, 501 (1992) http://dx.doi.org/10.1104/pp.98.2.501[Crossref]
  • [30] X. Wang, G. Zhao, H. Gu, Afr. J. Biotechnol. 8, 5773 (2009)
  • [31] S.M. Bian, Y.W. Jiang, Sci. Hortic. 120, 264 (2009) http://dx.doi.org/10.1016/j.scienta.2008.10.014[Crossref]
  • [32] Y. Tan, Z. Liang, H. Shao, F. Du, Colloid. Surface. B. 49, 60 (2006) http://dx.doi.org/10.1016/j.colsurfb.2006.02.014[Crossref]
  • [33] H. Kai, L. Zongsuo, D. Qimei, Acta Agr. Bor.-Occident. Sinica 5, 100 (2010)
  • [34] N. Navrot et al., Plant Physiol. 142, 1364 (2006) http://dx.doi.org/10.1104/pp.106.089458[Crossref]
  • [35] Y. Miao et al., Plant Cell 18, 2749 (2006) http://dx.doi.org/10.1105/tpc.106.044230[Crossref]
  • [36] D.I. Štajner, B.M. Popović, P. BoŽa, A. Kapor, Phytother. Res. 23, 1006 (2009) http://dx.doi.org/10.1002/ptr.2741[Crossref]
  • [37] L. Saleh, C. Plieth, Plant Methods 5, 1 (2009) http://dx.doi.org/10.1186/1746-4811-5-2[Crossref]
  • [38] S.R. Georgetti, R. Casagrande, V.M. Di Mambro, A.E.C.S. Azzolini, M.J.V. Fonseca, AAPS PharmSci 5, 1 (2003) http://dx.doi.org/10.1208/ps050220[Crossref]
  • [39] M. Kawagoe, K. Nakagawa, Toxicol. Lett. 114, 189 (2000) http://dx.doi.org/10.1016/S0378-4274(99)00296-9[Crossref]
  • [40] J. Asgarpanah, S.M. Motamed, A. Farzaneh, B. Ghanizadeh, S. Tomraee, Afr. J. Biotechnol. 10, 19176 (2011)
  • [41] J.G. Souza et al., Z. Naturforsch. C. 62, 339 (2007)
  • [42] B. Gonzalez Flecha, S. Llesuy, A. Boveris, Free Radical Bio. Med. 10, 93 (1991) http://dx.doi.org/10.1016/0891-5849(91)90002-K[Crossref]
  • [43] C. Desmarchelier, J. Coussio, G. Ciccia, Braz. J. Med. Biol. Res. 31, 1163 (1998) http://dx.doi.org/10.1590/S0100-879X1998000900010[Crossref]
  • [44] D.L. Miladinović et al., Anal. Bioanal. Chem. 403, 1007 (2012) http://dx.doi.org/10.1007/s00216-012-5866-1[Crossref]
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-012-0163-6
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