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Journal
2013 | 11 | 2 | 320-323
Article title

Rapid flow injection electrochemical detection of 3,3′,4,4′ tetrachlorobiphenyl using stabilized lipid membranes with incorporated sheep antibody

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EN
Abstracts
EN
An electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3′,4,4′ tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10−8 to 10−5 M, with a detection limit of ca. 10−8 M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5. [...]
Publisher

Journal
Year
Volume
11
Issue
2
Pages
320-323
Physical description
Dates
published
1 - 2 - 2013
online
29 - 11 - 2012
Contributors
  • Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece, nikolelis@chem.uoa.gr
  • Laboratory of Inorganic Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece
  • Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece
  • Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Dept 1, Chemical Sciences, National Technical University of Athens, GR-15780, Athens, Greece
  • Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-012-0157-4
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