A close analysis of metal-enhanced fluorescence of tryptophan induced by silver nanoparticles: wavelength emission dependence

• Authors: Anderson Caires , Luciano Costa , Joelson Fernandes
• Languages of publication: EN

Abstracts

EN

In the last few years, silver nanoparticles have been proposed as a promising alternative for the label-free detection of proteins via metal-enhanced fluorescence. Generally, the aromatic amino acid tryptophan is most frequently used in this type of studies, because the intrinsic fluorescence of proteins is usually dominated by tryptophan emissions. In the present study, we evaluated the fluorescence behavior of tryptophan in the presence of a silver colloid with nanoparticles of 100 nm in diameter. The results showed that a nanoparticles concentration of 32 mg L−1 induced maximum fluorescence enhancement. However, the metal-enhanced fluorescence was dependent on the emission wavelength of tryptophan, and this phenomenon was closely related to the metal surface reabsorption process (inner filter effect), suggesting that the plasmon resonance reabsorption effect should be taken into account in analyses involving protein studies by metal-enhanced fluorescence.

Keywords

EN

Plasmon resonance; Silver nanoparticle; Protein; Metal-enhanced fluorescence; Tryptophan

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 1
• Pages: 111-115

Dates

published

1 - 1 - 2013

online

24 - 10 - 2012

Contributors

author

Anderson Caires

• Federal University of Grande Dourados — UFGD

author

Luciano Costa

• Federal University of Grande Dourados — UFGD

author

Joelson Fernandes

• Federal University of Grande Dourados — UFGD

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Identifiers

DOI

10.2478/s11532-012-0139-6