Simultaneous analysis of first-line anti-tuberculosis drugs in tablets by UV spectrophotometry compared to capillary zone electrophoresis

• Authors: Luiza Marcellos , Adriana Faria , Marcus Souza , Mariana Almeida , Guilherme Sabin , Ronei Poppi , Marcone Oliveira
• Languages of publication: EN

Abstracts

EN

The development and optimization of a novel UV spectrophotometric methodology was proposed for simultaneous analysis of ethambutol (ETB), isoniazid (ISO), rifampicin (RIF) and pyrazinamide (PYR), using multivariate calibration based on the partial least squares method (PLS). The methodology was successfully applied for analysis of four-drug fixed dose combination (4-FDC) tablets used for tuberculosis treatment. A 34 Box-Behnken design, with triplicate in central point, was used for sample preparation in the calibration step. In the present case, nine latent variables were chosen for the model development that presented the smallest RMSECV and explain 98.76% of data variance in Y block (concentrations of ETB ISO, RIF and PYR) and 99.93% of data variance in X block (spectral data). PLS models for ETB, ISO, RIF and PYR presented RMSEP and R2 values of 0.23 mg L−1 and 0.971; 0.14 mg L−1 and 0.731; 0.11 mg L−1 and 0.990 and 0.57 mg L−1 and 0.972, respectively. A validation step was performed based on the comparison between the UV spectrophotometric proposed methodology and capillary zone electrophoresis (CZE) in 4-FDC real samples and no significant difference was found between two methodologies at 95% of confidence level. [...]

Keywords

EN

4-FDC; UV spectrophotometry; Chemometrics; Capillary electrophoresis

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2012
• Volume: 10
• Issue: 6
• Pages: 1808-1816

Dates

published

1 - 12 - 2012

online

21 - 9 - 2012

Contributors

author

Luiza Marcellos

• Department of Chemistry — ICE, Federal University of Juiz de Fora, CEP 36036-330, Juiz de Fora — MG, Brazil

author

Adriana Faria

• Department of Chemistry — ICE, Federal University of Juiz de Fora, CEP 36036-330, Juiz de Fora — MG, Brazil

author

Marcus Souza

• Institute of Technology in Pharmaceuticals — Farmanguinhos, Oswaldo Cruz Foundation, CEP 21041-250, Rio de Janeiro — RJ, Brazil

author

Mariana Almeida

• Institute of Chemistry, State University of Campinas, CEP 13084-971, Campinas — SP, Brazil

author

Guilherme Sabin

• Institute of Chemistry, State University of Campinas, CEP 13084-971, Campinas — SP, Brazil

author

Ronei Poppi

• Institute of Chemistry, State University of Campinas, CEP 13084-971, Campinas — SP, Brazil

author

Marcone Oliveira

• Department of Chemistry — ICE, Federal University of Juiz de Fora, CEP 36036-330, Juiz de Fora — MG, Brazil

References

• [1] M.V.N. de Souza, M.L. Ferreira, A.C. Pinheiro, M.F. Saraiva, M.V. de Almeida, M.S. Valle, The Scientific World Journal 8, 720 (2008) http://dx.doi.org/10.1100/tsw.2008.99[Crossref]
• [2] M.V.N. de Souza, Curr. Opin. Pulm. Med. 12, 167 (2006) http://dx.doi.org/10.1097/01.mcp.0000219264.42686.c9[Crossref]
• [3] M.V.N. de Souza, New Strategies Combating Bacterial Infection (Wiley-VCH, Weinheim, 2008) KGaA71
• [4] S.A. Carvalho, E.F. da Silva, M.V.N. de Souza, M.C.S. Lourenço, F.R. Vicente, Bioorg. Med. Chem. Lett. 18, 538 (2008) http://dx.doi.org/10.1016/j.bmcl.2007.11.091[Crossref]
• [5] M.V.N. de Souza, Fitoterapia 80, 453 (2009) http://dx.doi.org/10.1016/j.fitote.2009.07.010[Crossref]
• [6] S. Agrawal, K.J. Kaur, I. Singh, S.R. Bhade, C.L. Kaul, R. Panchangula, Int. J. Pharm. 233, 169 (2002) http://dx.doi.org/10.1016/S0378-5173(01)00939-5[Crossref]
• [7] S.A. Benetton, E.R.M. Kedor-Hackmann, M.I.R.M. Santoro, V.M. Borges, Talanta 47, 639 (1998) http://dx.doi.org/10.1016/S0039-9140(98)00111-8[Crossref]
• [8] H.C. Goicoechea, A.C. Olivieri, J. Pharm. Biomed. Anal. 20, 681 (1999) http://dx.doi.org/10.1016/S0731-7085(99)00072-2[Crossref]
• [9] A. Espinosa-Mansila, M.I.A. Valenzuela, A. Muñoz de Lapeña, F. Salinas, F.C. Cañada, Anal. Chim. Acta 427, 129 (2001) http://dx.doi.org/10.1016/S0003-2670(00)01195-8[Crossref]
• [10] J. Madan, A.K. Dwivedi, S. Singh, Anal. Chim. Acta 538, 345 (2005) http://dx.doi.org/10.1016/j.aca.2005.02.015[Crossref]
• [11] S.H. Songh, S.H. Jun, K.U. Park, Y. Yoon, J.H. Lee, J.Q. Kim, J. Song, Rapid Commun. MassSpectrom. 21, 1331 (2007) http://dx.doi.org/10.1002/rcm.2961[Crossref]
• [12] A.F. Faria, M.V.N. de Souza, R.E. Bruns, M.A.L. de Oliveira, Talanta 82, 333 (2010) http://dx.doi.org/10.1016/j.talanta.2010.04.044[Crossref]
• [13] A.F. Faria, M.V.N. de Souza, R.E. Bruns, M.A.L. de Oliveira, J. Chromatogr. A1202, 224 (2008)
• [14] A.F. Faria, L.F. Marcellos, J.P.M. Vasconcelos, M.V.N. de Souza, A.L.S. Júnior, W.R. do Carmo, R. Diniz, M.A.L. de Oliveira, J. Braz. Chem. Soc. 22, 867 (2011) http://dx.doi.org/10.1590/S0103-50532011000500008[Crossref]
• [15] A. Bartacek, D. Schütt, B. Panosch, M. Borek, Int. J. Tuberc. Lung Dis. 13(6), 760 (2009)
• [16] M. Koel, M. Kaljurand, Pure Appl. Chem. 78(11), 1993 (2006) http://dx.doi.org/10.1351/pac200678111993[Crossref]
• [17] USP 34 - The United States Pharmacopoeia (U.S. Pharmacopoeia Convention, Rockville, 2011) 3, 4154
• [18] D.L. Massart, B.G.M. Vandeginste, L.M.C. Buydens, S. De Jong, P.J Lewi, J. Smeyers-Verbeke, Handbook of chemometrics and qualimetrics (Elsevier, Amsterdam, 1997) part A, 187
• [19] K. Danzer, L.A. Currie, Pure Appl. Chem. 70, 993 (1998) http://dx.doi.org/10.1351/pac199870040993[Crossref]
• [20] A.F. Faria, M.V.N. de Souza, M.A.L. de Oliveira, J. Braz. Chem. Soc. 19, 389 (2008) http://dx.doi.org/10.1590/S0103-50532008000300004[Crossref]
• [21] D.R. Baker, Capillary electrophoresis (John Wiley & Sons, Inc, USA, 1995)

Identifiers

DOI

10.2478/s11532-012-0102-6