Use of an immiscible diluent in ionic liquid / ion pair LC for the assay of an injectable analgesic

• Authors: Toma Galaon , Medeea Radulescu , Victor David , Andrei Medvedovici
• Languages of publication: EN

Abstracts

EN

Injectable solutions used in treatment of intense pain are based on combinations of active ingredients such as metamizole sodium (MTZ), pitofenone hydrochloride (PTF) and fenpiverine bromide (FPB). The simultaneous chromatographic assay of such combinations poses difficulties due to their structural variety, highly polar character, and wide concentration ranges (500 mg mL−1 for MTZ, 2 mg mL−1 for PTF and 0.02 mg mL−1 for FPB). Fast hydrolysis of MTZ on aqueous dilution causes additional problems due to impurity (MTC) formation. Sodium hexane sulphonate (10 mM) was used as ion pairing agent for PTF, FPB and MTC in a mobile phase consisting of 48/52 (v/v) methanol and aqueous 0.2% triethylamine at pH=3. The ionic liquid 1-butyl-1-methyl-pyrrolidinium tetrafluoroborate (10 mM) was used as mobile phase additive to preserve the MTZ peak symmetry. The minor active ingredient FPB was selectively extracted into 1-octanol by ion pair formation with picric acid. A 20 µL aliquot of the organic layer was directly injected into the column. [...]

Keywords

EN

Ionic liquids addition; Ion-pair separation mechanism; Injection of immiscible diluent; Analgesic combination

• Journal: Open Chemistry
• Year: 2012
• Volume: 10
• Issue: 4
• Pages: 1360-1368

Dates

published

1 - 8 - 2012

online

29 - 5 - 2012

Contributors

author

Toma Galaon

• Faculty of Chemistry, Department of Analytical Chemistry, University of Bucharest, Bucharest, 50663, Romania

author

Medeea Radulescu

• Faculty of Chemistry, Department of Analytical Chemistry, University of Bucharest, Bucharest, 50663, Romania

author

Victor David

• Faculty of Chemistry, Department of Analytical Chemistry, University of Bucharest, Bucharest, 50663, Romania

author

Andrei Medvedovici

• Faculty of Chemistry, Department of Analytical Chemistry, University of Bucharest, Bucharest, 50663, Romania,

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Identifiers

DOI

10.2478/s11532-012-0059-5