A study of the antioxidative behavior of phenolic acids, in aqueous herb extracts, using a dsDNA biosensor

• Authors: Elina Skeva , Stella Girousi
• Languages of publication: EN

Abstracts

EN

Electrochemical DNA biosensors are promising tools for the fast, inexpensive and simple in vitro analysis for the determination of free radicals and antioxidants. High concentrations of antioxidants in such compounds as phenolic acids and plant extracts, act as free radical terminators which reduce the effect of the oxidative dam-age on DNA. The electrochemical behavior of three representative phenolic acids, caffeic acid, gallic acid and trolox were studied by cyclic voltammetry. Moreover, the determination of the above antioxidants under the optimized conditions (scan rate, deposition potential and time) using differential pulse voltammetry was also investigated. In vitro studies focused on their antioxidative effect were performed by adsorptive transfer stripping voltammetry and dsDNA biosensor. Using Fenton’s system, with FeSO4 and H2O2 was chosen as a strong oxidative system. This biosensor was applied as a screening antioxidant test in order to estimate the antioxidant capacity of aqueous herb extracts. [...]

Keywords

EN

Phenolic acids; Voltammetry; dsDNA electrochemical biosensor; Screening antioxidant test; Herbs

• Journal: Open Chemistry
• Year: 2012
• Volume: 10
• Issue: 4
• Pages: 1280-1289

Dates

published

1 - 8 - 2012

online

29 - 5 - 2012

Contributors

author

Elina Skeva

• Analytical Chemistry Laboratory, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece

author

Stella Girousi

• Analytical Chemistry Laboratory, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece,

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Identifiers

DOI

10.2478/s11532-012-0051-0