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Journal
2012 | 10 | 4 | 1280-1289
Article title

A study of the antioxidative behavior of phenolic acids, in aqueous herb extracts, using a dsDNA biosensor

Content
Title variants
Languages of publication
EN
Abstracts
EN
Electrochemical DNA biosensors are promising tools for the fast, inexpensive and simple in vitro analysis for the determination of free radicals and antioxidants. High concentrations of antioxidants in such compounds as phenolic acids and plant extracts, act as free radical terminators which reduce the effect of the oxidative dam-age on DNA. The electrochemical behavior of three representative phenolic acids, caffeic acid, gallic acid and trolox were studied by cyclic voltammetry. Moreover, the determination of the above antioxidants under the optimized conditions (scan rate, deposition potential and time) using differential pulse voltammetry was also investigated. In vitro studies focused on their antioxidative effect were performed by adsorptive transfer stripping voltammetry and dsDNA biosensor. Using Fenton’s system, with FeSO4 and H2O2 was chosen as a strong oxidative system. This biosensor was applied as a screening antioxidant test in order to estimate the antioxidant capacity of aqueous herb extracts. [...]
Publisher

Journal
Year
Volume
10
Issue
4
Pages
1280-1289
Physical description
Dates
published
1 - 8 - 2012
online
29 - 5 - 2012
Contributors
author
  • Analytical Chemistry Laboratory, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece
  • Analytical Chemistry Laboratory, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece, girousi@chem.auth.gr
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-012-0051-0
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