Altered membrane amino acids composition in human colorectal cancer tissue

• Authors: Barbara Szachowicz-Petelska , Stanisław Sulkowski , Zbigniew Figaszewski
• Languages of publication: EN

Abstracts

EN

Cancer transformation is characterized by changes in cell metabolism, which can alter the structure and function of cell membrane components, including integral membrane proteins. Qualitative and quantitative estimations of integral membrane protein are necessary for studies aimed at understanding their modifications under pathological conditions. Herein, we used a high-performance liquid chromatography (HPLC)-based approach that involved selective hydrolysis of isolated tissue cell membrane proteins to peptides, resolution by chromatography and determination of the amino acid content (phenylalanine (Phe), tyrosine (Tyr), cysteine (Cys) and lysine (Lys)) in individual peptides. The results demonstrate decrease in peptide levels and their amino acids content in integral membrane proteins in human colorectal cancer tissue. Therefore, cancer transformation causes a decrease in the levels of integral membrane proteins, which may in turn lead to an increase in the levels of other charged molecules on the cell surface, such as phospholipids. It might lead to the reconstruction and functional rearrangement of the cell membrane, for example: the permeability, electric properties, fluidity etc. [...]

Keywords

EN

Amino acids; Peptides; Integral membrane proteins; HPLC; Colorectal cancer

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2012
• Volume: 10
• Issue: 4
• Pages: 1245-1252

Dates

published

1 - 8 - 2012

online

29 - 5 - 2012

Contributors

author

Barbara Szachowicz-Petelska

• Institute of Chemistry, University in Białystok, 15-443, Białystok, Poland

author

Stanisław Sulkowski

• Department of General Pathomorphology, Medical University of Białystok, 15-269, Białystok, Poland

author

Zbigniew Figaszewski

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Identifiers

DOI

10.2478/s11532-012-0050-1