Non-steroidal anti-inflammatory drugs, as a therapeutic class, are among the most often used active pharmaceutical ingredients in heath care in the world. They are mostly available without prescription and often used for treatment of fever and pain. An extensive research of the literature published in analytical and pharmaceutical chemistry journals has been conducted and the chromatographic methods which were used for the purity, stability and pharmacokinetic studies of the cyclooxygenase-2 inhibitors, in formulations and biological materials have been reviewed. The methodology for the analysis of selected drugs is very well documented and many examples are available in the literature. The common use of chromatographic techniques with various detection attachments provide possibility for monitoring of drugs in therapy.