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Number of results

Journal

2012 | 10 | 4 | 1073-1078

Article title

Impact of un-polymerized acrylamide monomer residues onto protein identification by MALDI TOF MS

Content

Title variants

Languages of publication

EN

Abstracts

EN
Polyacrylamide gel electrophoresis is a powerful tool for protein mixture separation frequently used in proteomic studies. This article describes the problems which can arise during the identification process of separated proteins by matrix assisted laser desorption/ionization mass spectrometry. Presence of residual acrylamide monomers in the peptide samples leads to a significant peptide signal suppression. In the case of protein prohibitin, isolated from the MCF 7 breast cancer cell line, the peptide signal suppression was observed in 50% of all detected peptides. [...]

Publisher

Journal

Year

Volume

10

Issue

4

Pages

1073-1078

Physical description

Dates

published
1 - 8 - 2012
online
29 - 5 - 2012

Contributors

author
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
author
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_s11532-012-0024-3
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